Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,464,372	C→A	M212I (ATG→ATT)	mlaA ←	intermembrane phospholipid transport system ‑ outer membrane lipoprotein MlaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,464,372	0	C	A	100.0%	198.0 / NA	57	M212I (ATG→ATT)	mlaA	intermembrane phospholipid transport system ‑ outer membrane lipoprotein MlaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/26);  total (31/26)

TTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGT  >  NC_000913/2464239‑2464499                                                                                                                                      |                                                                                                                                ttttATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAataata                                                                                                                           <  1:466932/140‑1 (MQ=255)      tttGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAgg                                                                                                                      >  1:595962/1‑140 (MQ=255)        tGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTTGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGAt                                                                                                                    >  2:943468/1‑140 (MQ=255)        tGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGAt                                                                                                                    >  1:670822/1‑140 (MQ=255)            tCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGga                                                                                                                >  1:674835/1‑140 (MQ=255)                  tCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTg                                                                                                          <  1:707865/140‑1 (MQ=255)                   cAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGa                                                                                                         <  1:167707/140‑1 (MQ=255)                   cAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGa                                                                                                         <  2:373950/140‑1 (MQ=255)                   cAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGa                                                                                                         <  2:305899/140‑1 (MQ=255)                      aaTCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGa                                                                                                         >  2:254792/1‑137 (MQ=255)                      aaTCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGa                                                                                                         <  1:254792/137‑1 (MQ=255)                      aaTCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACgc                                                                                                      >  2:984784/1‑140 (MQ=255)                      aaTCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGCATCGGACGACTGACgc                                                                                                      >  2:1013107/1‑140 (MQ=255)                             atCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGAc                                                                                               <  2:14410/140‑1 (MQ=255)                                ttttAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCAt                                                                                            >  2:230671/1‑140 (MQ=255)                                ttttAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCAt                                                                                            >  1:862967/1‑140 (MQ=255)                                ttttAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCAt                                                                                            >  1:261174/1‑140 (MQ=255)                                  ttAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGAc                                                                                               >  1:950275/1‑135 (MQ=255)                                  ttAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGAc                                                                                               <  2:950275/135‑1 (MQ=255)                                  ttAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCg                                                                                          >  2:287600/1‑140 (MQ=255)                                     aaTCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGaa                                                                                       <  1:230671/140‑1 (MQ=255)                                          tcCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCcag                                                                                  >  1:1043065/1‑140 (MQ=255)                                          tcCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCcag                                                                                  >  1:1039330/1‑140 (MQ=255)                                          tcCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCcag                                                                                  >  1:176259/1‑140 (MQ=255)                                          tcCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCcag                                                                                  >  1:334352/1‑140 (MQ=255)                                           ccTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCcagc                                                                                 >  2:582009/1‑140 (MQ=255)                                                    cTTGTGCGTTCTGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCCATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATATGCATCGTATGACTGACGCAGCAGACCATCGGAACCCAGCAGTTGAgcg                                                                        >  2:611316/1‑140 (MQ=255)                                                    cTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAgcg                                                                        >  2:372757/1‑140 (MQ=255)                                                    cTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAgcg                                                                        >  1:436368/1‑140 (MQ=255)                                                    cTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAgcg                                                                        >  2:451431/1‑140 (MQ=255)                                                    cTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAgcg                                                                        >  1:326357/1‑140 (MQ=255)                                                    cTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATG‑AATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAgcgc                                                                       >  2:577960/1‑140 (MQ=255)                                                     ttGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAgcgc                                                                       <  2:790329/140‑1 (MQ=255)                                                     ttGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAgcgc                                                                       <  1:291468/140‑1 (MQ=255)                                                      tgtgCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGcgcg                                                                      >  1:364390/1‑140 (MQ=255)                                                           gTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGttt                                                                 >  2:914430/1‑140 (MQ=255)                                                            ttCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTc                                                                >  1:786663/1‑140 (MQ=255)                                                             tCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCg                                                               <  2:436368/140‑1 (MQ=255)                                                             tCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCg                                                               <  2:595962/140‑1 (MQ=255)                                                              cGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGa                                                              <  2:230256/140‑1 (MQ=255)                                                              cGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGa                                                              <  1:171603/140‑1 (MQ=255)                                                                     tCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTc                                                       <  1:451431/140‑1 (MQ=255)                                                                       cTGCGGTTTGAGTTCGCCGCCATTAGCGATG‑AATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAg                                                    <  1:577960/140‑1 (MQ=255)                                                                         gCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGc                                                   <  1:287600/140‑1 (MQ=255)                                                                                gAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCAtt                                            <  2:326357/140‑1 (MQ=255)                                                                                 aGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCAttt                                           >  2:1006823/1‑140 (MQ=255)                                                                                      gccgccATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCca                                      <  2:1043065/140‑1 (MQ=255)                                                                                           cATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGAc                                 >  2:584611/1‑140 (MQ=255)                                                                                                 cGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGc                           <  2:862967/140‑1 (MQ=255)                                                                                                          cATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGc                  <  2:334352/140‑1 (MQ=255)                                                                                                          cATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGc                  <  2:1035380/140‑1 (MQ=255)                                                                                                          cATGACGCTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGc                  <  1:611316/140‑1 (MQ=255)                                                                                                                 cTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCgg                            >  2:724008/1‑123 (MQ=255)                                                                                                                 cTGGAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCgg                            <  1:724008/123‑1 (MQ=255)                                                                                                                    gAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGc                  <  2:165553/130‑1 (MQ=255)                                                                                                                    gAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGc                  >  1:165553/1‑130 (MQ=255)                                                                                                                    gAAGTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGaa        <  2:786663/140‑1 (MQ=255)                                                                                                                       gTACGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCg     >  1:298698/1‑140 (MQ=255)                                                                                                                          cGCTTCGCGCACAATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGt  >  1:617017/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                TTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAATATAAGGATCGGACGACTGACGCAGCAGACCATCGGAATCCAGCAGCTGAGCGCGGGTTTCGATCCCTTCAAGCGTCCATTTACCCACAGACATCGGCCAGGTCAGCCAGGAAAGAACCGGGT  >  NC_000913/2464239‑2464499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑