Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,481,363	G→T	R272S (CGT→AGT)	emrY ←	tripartite efflux pump membrane subunit EmrY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,481,363	0	G	T	100.0%	292.3 / NA	82	R272S (CGT→AGT)	emrY	tripartite efflux pump membrane subunit EmrY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (34/48);  total (34/48)

GCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGAACCAATCCAGATCGCG  >  NC_000913/2481231‑2481495                                                                                                                                     |                                                                                                                                     gcgcATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTa                                                                                                                               >  1:413457/1‑140 (MQ=255)    cATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTaaac                                                                                                                            >  1:631308/1‑140 (MQ=255)       aaGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTaaacaaa                                                                                                                         <  2:667469/140‑1 (MQ=255)         gCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACt                                                                                                                       <  1:479471/140‑1 (MQ=255)          cAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTg                                                                                                                      <  2:713945/140‑1 (MQ=255)                 ggCCCCTAACCCAATATTCCCCATCGTTTCCTAGAAGAACTGGGGCATAAGGGCGAACCCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCaa                                                                                                               <  2:420347/140‑1 (MQ=255)                   cccATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGa                                                                                                             <  2:631308/140‑1 (MQ=255)                       tatCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCg                                                                                                         >  2:255128/1‑140 (MQ=255)                       tatCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCg                                                                                                         >  1:767811/1‑140 (MQ=255)                       tatCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCg                                                                                                         >  1:530656/1‑140 (MQ=255)                        atCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCgg                                                                                                        >  1:10145/1‑140 (MQ=255)                         tCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCggg                                                                                                       <  2:767811/140‑1 (MQ=255)                         tCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCggg                                                                                                       <  2:97061/140‑1 (MQ=255)                         tCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCggg                                                                                                       <  1:144699/140‑1 (MQ=255)                         tCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCggg                                                                                                       <  2:313286/140‑1 (MQ=255)                             aTTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTtct                                                                                                   >  2:613940/1‑140 (MQ=255)                                  aCCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAg                                                                                              >  1:813654/1‑140 (MQ=255)                                     ccATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTc                                                                                           <  1:550198/140‑1 (MQ=255)                                     ccATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTc                                                                                           <  1:897663/140‑1 (MQ=255)                                      cATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCCTGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCg                                                                                          >  2:703957/1‑140 (MQ=255)                                      cATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCg                                                                                          >  2:886301/1‑140 (MQ=255)                                        tCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGAc                                                                                        <  1:555099/140‑1 (MQ=255)                                             tCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCa                                                                                   <  2:104407/140‑1 (MQ=255)                                                tGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAAt                                                                                <  2:671755/140‑1 (MQ=255)                                                       aCTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTaaa                                                                         >  1:21948/1‑140 (MQ=255)                                                       aCTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTaaa                                                                         <  1:867896/140‑1 (MQ=255)                                                       aCTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTaaa                                                                         >  1:803873/1‑140 (MQ=255)                                                              cATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCa                                                                  <  2:438328/140‑1 (MQ=255)                                                                tAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCaga                                                                >  1:509504/1‑140 (MQ=255)                                                                   ggACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTGGACTCCCAAATGACTAAAGAGATCAGAGaa                                                             <  2:21653/140‑1 (MQ=255)                                                                   ggACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTa                                                                           >  2:776667/1‑126 (MQ=255)                                                                   ggACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTa                                                                           <  1:776667/126‑1 (MQ=255)                                                                   ggACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGaa                                                             <  1:886301/140‑1 (MQ=255)                                                                     aCGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAAt                                                           <  2:424364/140‑1 (MQ=255)                                                                      cGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATa                                                          <  1:837007/140‑1 (MQ=255)                                                                           gCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGa                                                     <  2:21948/140‑1 (MQ=255)                                                                            cTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGAt                                                    >  1:876914/1‑140 (MQ=255)                                                                            cTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGAt                                                    >  2:618280/1‑140 (MQ=255)                                                                             tCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGAta                                                   <  2:530656/140‑1 (MQ=255)                                                                             tCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGAta                                                   <  1:613940/140‑1 (MQ=255)                                                                              ccAGAGTAAAATAAATACGCGCCTGTGATACTCACAATCCCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGAtac                                                  <  1:703957/140‑1 (MQ=255)                                                                               cAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGAtact                                                 <  1:618280/140‑1 (MQ=255)                                                                                agagTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGAtacta                                                <  2:803873/140‑1 (MQ=255)                                                                                agagTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGAtacta                                                <  2:256493/140‑1 (MQ=255)                                                                                 gagTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGAtactac                                               <  2:509504/140‑1 (MQ=255)                                                                                     aaaaTAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGtt                                           <  2:107566/140‑1 (MQ=255)                                                                                     aaaaTAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGtt                                           <  2:361642/140‑1 (MQ=255)                                                                                     aaaaTAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGtt                                           <  1:490188/140‑1 (MQ=255)                                                                                       aataaataCGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGtt                                           >  1:1028645/1‑138 (MQ=255)                                                                                       aataaataCGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGtt                                           <  2:1028645/138‑1 (MQ=255)                                                                                       aataaataCGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTaa                                         <  1:88430/140‑1 (MQ=255)                                                                                       aataaataCGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTaa                                         <  2:475758/140‑1 (MQ=255)                                                                                            ataCGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAAtatta                                    <  1:255128/140‑1 (MQ=255)                                                                                             taCGCGCATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAAtattat                                   <  2:413457/140‑1 (MQ=255)                                                                                                 cgcATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTaa                                         <  1:57069/130‑1 (MQ=255)                                                                                                 cgcATGTGATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTaa                                         >  2:57069/1‑130 (MQ=255)                                                                                                     tgtgATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTaa                                         >  1:882059/1‑126 (MQ=255)                                                                                                     tgtgATACTCACAATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTaa                                         <  2:882059/126‑1 (MQ=255)                                                                                                              cacaATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGa                  >  1:958257/1‑140 (MQ=255)                                                                                                               acaATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGaa                 <  2:10145/140‑1 (MQ=255)                                                                                                               acaATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGaa                 >  2:754630/1‑140 (MQ=255)                                                                                                               acaATACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGaa                 <  1:754630/140‑1 (MQ=255)                                                                                                                 aaTACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGAAcc               >  2:1033104/1‑140 (MQ=255)                                                                                                                   tACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACt                          <  2:77117/127‑1 (MQ=255)                                                                                                                   tACCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACt                          >  1:77117/1‑127 (MQ=255)                                                                                                                    aCCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGaa                 >  2:509655/1‑135 (MQ=255)                                                                                                                    aCCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGaa                 <  1:509655/135‑1 (MQ=255)                                                                                                                    aCCAATGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGAACCAAt            >  2:139002/1‑140 (MQ=255)                                                                                                                        aTGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGAACCAATCCAg        >  2:8922/1‑140 (MQ=255)                                                                                                                        aTGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGA‑‑‑TACTGTTAATATTATTATTGTACTCGAGt                     <  1:645548/124‑1 (MQ=255)                                                                                                                        aTGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGA‑‑‑TACTGTTAATATTATTATTGTACTCGAGt                     >  2:645548/1‑124 (MQ=255)                                                                                                                         tGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGAACCAATCCAGa       >  1:854106/1‑140 (MQ=255)                                                                                                                         tGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGAACCAATCCAGa       >  1:145039/1‑140 (MQ=255)                                                                                                                         tGGTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGAACCAATCCAGa       >  2:249212/1‑140 (MQ=255)                                                                                                                           gTGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGAACCAATCCAGATc     >  2:560927/1‑140 (MQ=255)                                                                                                                            tGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGAACCAATCCAGATcg    <  2:854106/140‑1 (MQ=255)                                                                                                                            tGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGAACCAATCCAGATcg    <  1:139002/140‑1 (MQ=255)                                                                                                                            tGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGAACCAATCCAGATcg    <  2:958257/140‑1 (MQ=255)                                                                                                                            tGAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGAACCAATCCAGATcg    <  1:1033104/140‑1 (MQ=255)                                                                                                                             gAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTg                   <  1:333623/124‑1 (MQ=255)                                                                                                                             gAAGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTg                   >  2:333623/1‑124 (MQ=255)                                                                                                                              aaGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGAACCAATCCAGATcgcg  >  2:1031846/1‑140 (MQ=255)                                                                                                                              aaGTTACTGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGAACCAATCCAGATcgcg  >  1:24825/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                     GCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGAACCAATCCAGATCGCG  >  NC_000913/2481231‑2481495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑