Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,487,336	C→A	A988D (GCC→GAC)	evgS →	sensory histidine kinase EvgS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,487,336	0	C	A	100.0%	191.2 / NA	55	A988D (GCC→GAC)	evgS	sensory histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/34);  total (21/34)

ACTGTCGAGGCAAAAGCAGAACAACCCATCACACTACCTGAAAAGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGCCACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGACTCGCAAACTCCGTGAGCAAAATTCTTCCTTAC  >  NC_000913/2487203‑2487467                                                                                                                                      |                                                                                                                                    aCTGTCGAGGCAAAAGCAGAACAACCCATCACACTACCTGAAAAGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGa                                                                                                                               <  2:594016/140‑1 (MQ=255)  cTGTCGAGGCAAAAGCAGAACAACCCATCACACTACCTGAAAAGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGAt                                                                                                                              <  2:947127/140‑1 (MQ=255)   tGTCGAGGCAAAAGCAGAACAACCCATCACACTACCTGAAAAGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATg                                                                                                                             <  1:801182/140‑1 (MQ=255)         ggCAAAAGCAGAACAACCCATCACACTACCTGAAAAGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGc                                                                                                                       <  2:721480/140‑1 (MQ=255)         ggCAAAAGCAGAACAACCCATCACACTACCTGAAAAGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGc                                                                                                                       <  1:889755/140‑1 (MQ=255)                    aacaacCCATCACACTACCTGAAAAGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTacac                                                                                                            <  2:74109/140‑1 (MQ=255)                    aacaacCCATCACACTACCTGAAAAGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTacac                                                                                                            >  1:425429/1‑140 (MQ=255)                           cATCACACTACCTGAAAAGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCa                                                                                                     >  2:503945/1‑140 (MQ=255)                               acacTACCTGAAAAGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAA‑CTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATg                                                                                                <  2:242363/140‑1 (MQ=255)                                cacTACCTGAAAAGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATg                                                                                                <  2:425429/140‑1 (MQ=255)                                cacTACCTGAAAAGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATg                                                                                                >  2:382029/1‑140 (MQ=255)                                cacTACCTGAAAAGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATg                                                                                                <  1:885606/140‑1 (MQ=255)                                cacTACCTGAAAAGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATg                                                                                                <  1:546007/140‑1 (MQ=255)                                  cTACCTGAAAAGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCa                                                                                              <  1:655354/140‑1 (MQ=255)                                    aCCTGAAAAGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAAc                                                                                            >  2:989773/1‑140 (MQ=255)                                          aaaGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATg                                                                                      <  1:823359/140‑1 (MQ=255)                                            aGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGAt                                                                                    <  1:389823/140‑1 (MQ=255)                                            aGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGAt                                                                                    <  2:726631/140‑1 (MQ=255)                                            aGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGAt                                                                                    <  1:87921/140‑1 (MQ=255)                                            aGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGAGGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGAt                                                                                    <  2:145351/140‑1 (MQ=255)                                            aGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATAATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGAt                                                                                    <  2:755639/140‑1 (MQ=255)                                             gTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATg                                                                                                <  2:505510/127‑1 (MQ=255)                                             gTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATg                                                                                                >  1:505510/1‑127 (MQ=255)                                             gTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGAt                                                                                    <  1:851667/139‑1 (MQ=255)                                             gTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGAt                                                                                    >  2:851667/1‑139 (MQ=255)                                               tGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTg                                                                                 >  1:1031803/1‑140 (MQ=255)                                                 aGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCt                                                                               <  2:903686/140‑1 (MQ=255)                                                   cATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCtta                                                                             <  2:915766/140‑1 (MQ=255)                                                      atTAATCGCGGATGATCATTTGACGAACAGGCTATTTCTCAAACGCCAGCTAAATCTATGAGGATATGATGTTGATGAAGATACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCttattt                                                                          <  2:405885/140‑2 (MQ=255)                                                       ttAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTAc                                                                         <  2:639007/140‑1 (MQ=255)                                                        tAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACt                                                                        >  1:194232/1‑140 (MQ=255)                                                              cgGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGtt                                                                  >  1:871554/1‑140 (MQ=255)                                                                gatgatCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTaa                                                                >  1:859327/1‑140 (MQ=255)                                                                    atcatcCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATg                                                            <  2:603920/140‑1 (MQ=255)                                                                     tcatcCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGc                                                           <  1:617421/140‑1 (MQ=255)                                                                               aaCAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATatgga                                                 <  2:574365/140‑1 (MQ=255)                                                                               aaCAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATatgga                                                 <  1:382029/140‑1 (MQ=255)                                                                               aaCAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATatgga                                                 <  1:75341/140‑1 (MQ=255)                                                                                        ttACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGa                                        >  1:1889/1‑140 (MQ=255)                                                                                             cAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGa                                   <  1:503945/140‑1 (MQ=255)                                                                                              aaaCGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGAc                                  >  1:187669/1‑140 (MQ=255)                                                                                                ccGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGACTc                                >  1:543334/2‑140 (MQ=255)                                                                                                aCGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGACTc                                >  1:879050/1‑140 (MQ=255)                                                                                                     aGCTAAATCTATTAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGACTCGCaaa                           >  1:572308/1‑140 (MQ=255)                                                                                                                ttAGGATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGACTCGCAAACTCCGTGAGCa                >  1:449826/1‑140 (MQ=255)                                                                                                                    gATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGACTCGCAAACTCCGTGAGCAAAAt            >  2:207298/1‑140 (MQ=255)                                                                                                                    gATATGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGACTCGCAAACTCCGTGAGCAAAAt            <  2:871554/140‑1 (MQ=255)                                                                                                                     atatGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGACTCGCAAACTCCGTGAGCAAAAtt           <  2:253517/140‑1 (MQ=255)                                                                                                                     atatGATGTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGACTCGCAAACTCCGTGAGCAAAAtt           <  2:859327/140‑1 (MQ=255)                                                                                                                        tgatgTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGACTCGCAAACTCCGTGAGCAAAAttctt        >  1:465986/1‑140 (MQ=255)                                                                                                                          atgTTGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGACTCGCAAACTCCGTGAGCAAAATTCTTcc      <  1:207298/140‑1 (MQ=255)                                                                                                                             ttGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGACTCGCAAACTCCGTGAGCAAAATTCTTCCTTa   <  2:572308/140‑1 (MQ=255)                                                                                                                             ttGATGAAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGACTCGCAAACTCCGTGAGCAAAATTCTTCCTTa   >  1:130988/1‑140 (MQ=255)                                                                                                                              tgatgaAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGACTCGCAAACTCCGTGAGCAAAATTCTTCCTTAc  >  2:204899/1‑140 (MQ=255)                                                                                                                                atgaAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGACTCGCAAACTCCGTGAGCAAAAtt           >  1:37289/1‑129 (MQ=255)                                                                                                                                atgaAGACACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGACTCGCAAACTCCGTGAGCAAAAtt           <  2:37289/129‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    ACTGTCGAGGCAAAAGCAGAACAACCCATCACACTACCTGAAAAGTTGAGCATATTAATCGCGGATGATCATCCGACCAACAGGCTATTACTCAAACGCCAGCTAAATCTATTAGGATATGATGTTGATGAAGCCACTGATGGTGTGCAAGCGCTACACAAAGTCAGTATGCAACATTATGATCTGCTTATTACTGACGTTAATATGCCGAATATGGATGGTTTTGAGTTGACTCGCAAACTCCGTGAGCAAAATTCTTCCTTAC  >  NC_000913/2487203‑2487467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑