Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,359,777	C→A	L194M (CTG→ATG)	gltD →	glutamate synthase subunit GltD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,359,777	0	C	A	100.0%	254.6 / NA	75	L194M (CTG→ATG)	gltD	glutamate synthase subunit GltD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/44);  total (31/44)

GCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCG  >  NC_000913/3359645‑3359912                                                                                                                                     |                                                                                                                                        gCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGaaaa                                                                                                                                  >  1:110392/1‑140 (MQ=255) gCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGaaaa                                                                                                                                  >  1:383481/1‑140 (MQ=255)                 gCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGt                                                                                                                  <  1:498694/140‑1 (MQ=255)                 gCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGt                                                                                                                  >  2:964924/1‑140 (MQ=255)                 gCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGt                                                                                                                  <  2:919776/140‑1 (MQ=255)                  cccGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTc                                                                                                                 <  2:110392/140‑1 (MQ=255)                   ccGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCg                                                                                                                <  1:898707/140‑1 (MQ=255)                      gCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCg                                                                                                             >  1:736449/1‑140 (MQ=255)                      gCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCg                                                                                                             >  2:210788/1‑140 (MQ=255)                        aGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTg                                                                                                           <  2:201946/140‑1 (MQ=255)                         ggTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGAcgcg                                                                                                                   >  2:76025/1‑131 (MQ=255)                         ggTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGAcgcg                                                                                                                   <  1:76025/131‑1 (MQ=255)                           tCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGaaa                                                                                                        >  2:781840/1‑140 (MQ=255)                            cTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAAt                                                                                                       <  2:94556/140‑1 (MQ=255)                                 gtgtGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCa                                                                                                  <  1:888570/140‑1 (MQ=255)                                  tgtgCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCAc                                                                                                 <  1:842198/140‑1 (MQ=255)                                    tgCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCg                                                                                               >  1:373733/1‑140 (MQ=255)                                    tgCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCg                                                                                               >  1:496854/1‑140 (MQ=255)                                    tgCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCg                                                                                               >  2:84498/1‑140 (MQ=255)                                     gCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCgg                                                                                              <  2:1023052/140‑1 (MQ=255)                                     gCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCgg                                                                                              >  1:666733/1‑140 (MQ=255)                                       ggATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCa                                                                                            <  1:400141/140‑1 (MQ=255)                                        gATGTCCTGACGCGTAACGGTGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCAt                                                                                           <  1:1050473/140‑1 (MQ=255)                                        gATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCAt                                                                                           <  1:964924/140‑1 (MQ=255)                                         aTGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATg                                                                                          >  1:269596/1‑140 (MQ=255)                                              cTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGc                                                                                             <  1:900375/132‑1 (MQ=255)                                              cTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATgg                                                                                         >  2:895568/1‑136 (MQ=255)                                              cTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATgg                                                                                         <  1:895568/136‑1 (MQ=255)                                              cTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGAATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGc                                                                                             >  2:900375/1‑132 (MQ=255)                                                gACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTg                                                                                   >  2:924467/1‑140 (MQ=255)                                                   gcgTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAAt                                                                                >  1:130098/1‑140 (MQ=255)                                                   gcgTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAAt                                                                                <  1:82551/140‑1 (MQ=255)                                                     gTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTc                                                                              <  2:269596/140‑1 (MQ=255)                                                     gTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTc                                                                              <  2:666733/140‑1 (MQ=255)                                                      tAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCgg                                                                                              <  2:52332/123‑1 (MQ=255)                                                      tAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCgg                                                                                              >  1:52332/1‑123 (MQ=255)                                                      tAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTAt                                                                                     >  2:847798/1‑132 (MQ=255)                                                      tAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTAt                                                                                     <  1:847798/132‑1 (MQ=255)                                                      tAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCa                                                                             <  1:893709/140‑1 (MQ=255)                                                      tAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCa                                                                             <  2:694762/140‑1 (MQ=255)                                                      tAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCa                                                                             >  1:694762/1‑140 (MQ=255)                                                      tAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCa                                                                             >  1:362932/1‑140 (MQ=255)                                                        aCGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGGCGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCaaa                                                                           <  1:1010763/140‑1 (MQ=255)                                                        aCGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCaaa                                                                           <  1:84498/140‑1 (MQ=255)                                                        aCGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCaaa                                                                           <  1:781840/140‑1 (MQ=255)                                                          ggCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACt                                                                         <  2:736449/140‑1 (MQ=255)                                                           gCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTc                                                                        <  2:373733/140‑1 (MQ=255)                                                               aaaaGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATa                                                                    <  2:383481/140‑1 (MQ=255)                                                                      gTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGt                                                             >  1:991600/1‑140 (MQ=255)                                                                      gTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGt                                                             >  1:487616/1‑140 (MQ=255)                                                                      gTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGt                                                             <  2:425715/140‑1 (MQ=255)                                                                       ttGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAgag                                                            >  2:243354/1‑138 (MQ=255)                                                                        tGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTgg                                                           >  1:140100/1‑140 (MQ=255)                                                                        tGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTgg                                                           >  1:822513/1‑140 (MQ=255)                                                                            ttCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCcg                                                       <  1:906026/140‑1 (MQ=255)                                                                                 ccGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACg                                                  <  2:140100/140‑1 (MQ=255)                                                                                  cGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGt                                                 <  1:210788/140‑1 (MQ=255)                                                                                  cGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGt                                                 <  2:117117/140‑1 (MQ=255)                                                                                    tCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGACATCTTCACCGGCATGGGTATTGCATTCAAACACAATACCGAAGTGGGCCGCGACGTAc                                               >  2:586960/1‑140 (MQ=255)                                                                                    tCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTAc                                               >  2:920740/1‑140 (MQ=255)                                                                                          aGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTgg                                         <  1:924467/140‑1 (MQ=255)                                                                                            aaaTTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTAAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGAc                                       <  1:77435/140‑1 (MQ=255)                                                                                              aTTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGa                                     <  1:483008/140‑1 (MQ=255)                                                                                                tGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGGCGATc                                   <  1:18770/140‑1 (MQ=255)                                                                                                  gCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATctg                                 <  1:243354/140‑1 (MQ=255)                                                                                                      gctgctGACCTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGa                             <  1:335384/140‑1 (MQ=255)                                                                                                               cTTCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACg                    <  2:496854/140‑1 (MQ=255)                                                                                                                ttCGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGa                   <  1:691875/140‑1 (MQ=255)                                                                                                                  cGGTATTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATg                 >  1:448622/1‑140 (MQ=255)                                                                                                                      aTTCCGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCgt             <  2:130098/140‑1 (MQ=255)                                                                                                                         ccGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGtt          <  2:842096/140‑1 (MQ=255)                                                                                                                         ccGGCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGtt          >  1:842096/1‑140 (MQ=255)                                                                                                                            gCCTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCt       >  2:204363/1‑140 (MQ=255)                                                                                                                             ccTTCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCtt      <  2:991600/140‑1 (MQ=255)                                                                                                                               ttCAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTgg    >  1:649002/1‑140 (MQ=255)                                                                                                                                 cAAGATGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCg  >  2:698433/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        GCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCG  >  NC_000913/3359645‑3359912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑