Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,468,881	G→T	P325T (CCA→ACA)	chiA ←	endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,468,881	0	G	T	100.0%	106.1 / NA	32	P325T (CCA→ACA)	chiA	endochitinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/10);  total (22/10)

TGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTT  >  NC_000913/3468744‑3469014                                                                                                                                          |                                                                                                                                      tGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTATCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCt                                                                                                                                     >  2:775849/1‑140 (MQ=255) tGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCt                                                                                                                                     >  2:941270/1‑140 (MQ=255) tGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCt                                                                                                                                     >  1:363243/1‑140 (MQ=255)   ggCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCg                                                                                                                                   >  2:257063/1‑140 (MQ=255)           ttAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATc                                                                                                                           <  1:941270/140‑1 (MQ=255)             aGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCg                                                                                                                         <  1:918727/140‑1 (MQ=255)             aGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCg                                                                                                                         <  2:453686/140‑1 (MQ=255)                  caacccaCCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTATCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGtt                                                                                                                    <  1:775849/140‑1 (MQ=255)                   aacccaCCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCt                                                                                                                                     >  2:329822/1‑122 (MQ=255)                   aacccaCCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTg                                                                                                                   >  2:480847/1‑140 (MQ=255)                    acccaCCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTgg                                                                                                                  >  2:461400/1‑140 (MQ=255)                       caccaGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGGGGt                                                                                                               <  2:101857/140‑1 (MQ=255)                         ccaGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGtt                                                                                                                              >  2:1046229/1‑123 (MQ=255)                         ccaGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGtt                                                                                                                              <  1:1046229/123‑1 (MQ=255)                         ccaGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGGGGTCa                                                                                                             >  1:902423/1‑140 (MQ=255)                                           aTTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGttt                                                                                                    <  2:652166/131‑1 (MQ=255)                                           aTTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGttt                                                                                                    >  1:652166/1‑131 (MQ=255)                                            ttACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTgg                                                                                          >  1:307333/1‑140 (MQ=255)                                                cATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCa                                                                                      <  1:461400/140‑1 (MQ=255)                                                  ttGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGTGGTCACCGGTGTTTCCCGAGTTGGATCAAc                                                                                    >  1:496392/1‑140 (MQ=255)                                                  ttGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGATGATGTCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAAc                                                                                    >  1:803337/1‑140 (MQ=255)                                                   tGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGtt                                                                                            <  1:272353/131‑1 (MQ=255)                                                   tGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGtt                                                                                            >  2:272353/1‑131 (MQ=255)                                                   tGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAAcc                                                                                   <  1:480847/140‑1 (MQ=255)                                                    gAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCg                                                                                  >  1:524648/1‑140 (MQ=255)                                                               ggTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCa                                                                       >  1:696296/1‑140 (MQ=255)                                                                 tAATTTTCGCTCCCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTGGGGGTCACCGGTTTTTCCGGAGTTGGATCAACCGGAGTCACAGCAcc                                                                     <  2:636140/140‑1 (MQ=255)                                                                   attttCGCTCCCTTCCTGGCCGCTCCACGCTTGAAATGAGTAATCGTTAACGCTACCCGTTGGTGTTGATGTCTCGCTGTTACCCGGAGTGGGGGTCCCGGGTTTTTCCGGAGTTGGATCACCCGGAGTCACAGCACCGc                                                                   <  2:114581/138‑1 (MQ=255)                                                                   aaTTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGc                                                                   >  1:357918/1‑140 (MQ=255)                                                                         gCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCattat                                                             >  1:12346/1‑140 (MQ=255)                                                                                       cGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGGGGTCACCGGTTTTTCCGGAGTTGGATCACCCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACg                                               <  2:902423/140‑1 (MQ=255)                                                                                       cGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACg                                               >  1:110615/1‑140 (MQ=255)                                                                                           ccACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAAc                                           >  2:257244/1‑140 (MQ=255)                                                                                              cGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAgg                                        >  1:305248/1‑140 (MQ=255)                                                                                                  tgaaaTGAGTAATCGTTACCGCTACCCGCTGGTGTTGATGTCTCGCTGTTACCGGGATTGGGGGCCCCCGGTTTTTCCGGAGTTGGACCACCGGGGGTCACGGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGcc                                    <  2:110615/137‑1 (MQ=255)                                                                                                    aaaTGAGTAATCGTTAACGCTACCCGCTGGTGTTGATGTCTCGCTTTTATCCGGAGTGGGGGCCCCCGGTTTTTCCGGAGTTGGATCACCCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAg                                  <  2:696296/139‑1 (MQ=255)                                                                                                                         aTCCGCTGGTGTTGATGTCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGAttt             >  1:808342/1‑140 (MQ=255)                                                                                                                                    ttGATGTCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTgttgccgtt  >  1:152065/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      TGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTT  >  NC_000913/3468744‑3469014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑