Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,531,387	G→T	G351G (GGC→GGA)	yhgE ←	putative transport protein YhgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,531,387	0	G	T	100.0%	196.8 / NA	57	G351G (GGC→GGA)	yhgE	putative transport protein YhgE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (36/21);  total (36/21)

ACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCACGCTAATGCGCATACTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTGCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGCGGTCGACAGTCCAGCCGTACTGA  >  NC_000913/3531250‑3531513                                                                                                                                          |                                                                                                                               aCCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCACGCTAATGCGCATACTGTCCAGCACCGGTGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTcc                                                                                                                              >  1:628119/1‑140 (MQ=255) aCCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCACGCTAATGCGCATACTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTcc                                                                                                                              <  1:427195/140‑1 (MQ=255)   cTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCACGCTAATGCGCATACTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCt                                                                                                                            >  1:987132/1‑140 (MQ=255)      gTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCACGCTAATGCGCATACTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTgaa                                                                                                                         <  2:854952/140‑1 (MQ=255)                 ttttGCCGCTCTGATAACGCGCCATATGGCTGTTCACGCTAATGCGCATACTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGa                                                                                                              >  2:595934/1‑140 (MQ=255)                      ccGCTCTGATAACGCGCCATATGGCTGTTCACGCTAATGCGCATACTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTg                                                                                                         <  2:987132/140‑1 (MQ=255)                      ccGCTCTGATAACGCGCCATATGGCTGTTCACGCTAATGCGCATACTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTg                                                                                                         <  1:21408/140‑1 (MQ=255)                                      ccATATGGCTGTTCACGCTAATGCGCATACTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACg                                                                                         >  1:890792/1‑140 (MQ=255)                                       cATATGGCTGTTCACGCTAATGCGCATACTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGa                                                                                        >  1:36883/1‑140 (MQ=255)                                       cATATGGCTGTTCACGCTAATGCGCATACTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACCTTTCCCTGAAGAACAACGGGATTTTGTCCTTTACGCCAGACGa                                                                                        >  1:717490/1‑140 (MQ=255)                                        atatGGCTGTTCACGCTAATGCGCATACTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGAt                                                                                       <  2:890792/140‑1 (MQ=255)                                        atatGGCTGTTCACGCTAATGCGCATACTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGAt                                                                                       >  2:318059/1‑140 (MQ=255)                                          atGGCTGTTCACGCTAATGCGCATACTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGc                                                                                     <  1:595934/140‑1 (MQ=255)                                             gCTGTTCACGCTAATGCGCATACTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGa                                                                                  >  1:522264/1‑140 (MQ=255)                                                 ttCACGCTAATGCGCATACTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACaaa                                                                              >  1:707190/1‑140 (MQ=255)                                                 ttCACGCTAATGCGCATACTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACaaa                                                                              >  1:788333/1‑140 (MQ=255)                                                 ttCACGCTAATGCGCATACTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACaaa                                                                              >  2:717332/1‑140 (MQ=255)                                                                  aCTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACcaca                                                             <  1:606950/140‑1 (MQ=255)                                                                  aCTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACcaca                                                             <  1:481414/140‑1 (MQ=255)                                                                    tGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACAcc                                                           >  2:163842/1‑140 (MQ=255)                                                                     gTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAAt                                                                            <  2:996897/122‑1 (MQ=255)                                                                     gTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAAt                                                                            >  1:996897/1‑122 (MQ=255)                                                                     gTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCa                                                          >  2:485978/1‑140 (MQ=255)                                                                      tCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAg                                                         >  1:627004/1‑140 (MQ=255)                                                                      tCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAg                                                         >  1:624015/1‑140 (MQ=255)                                                                       ccAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGt                                                        <  1:493078/140‑1 (MQ=255)                                                                              cGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCa                                                 >  2:753038/1‑140 (MQ=255)                                                                              cGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCa                                                 >  2:62864/1‑140 (MQ=255)                                                                                gCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCCCTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGc                                               >  1:639289/1‑140 (MQ=255)                                                                                gCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGc                                               >  1:130159/1‑140 (MQ=255)                                                                                gCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGc                                               >  2:271918/1‑140 (MQ=255)                                                                                         gaagCCCCAGTATCACCAACACCAATAACTAACCCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATTCCCCAGCGACCACACCAGTAACACCAGCACCGCCAGc                                      <  2:380091/140‑1 (MQ=255)                                                                                          aagCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATCCCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCa                                     <  2:522264/140‑1 (MQ=255)                                                                                             cACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGc                                  <  1:183695/140‑1 (MQ=255)                                                                                             cACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGc                                  >  2:684091/1‑140 (MQ=255)                                                                                              aCCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGcc                                 >  2:859461/1‑140 (MQ=255)                                                                                                    aTCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCa                                     <  1:583396/130‑1 (MQ=255)                                                                                                    aTCACCAACACCAATAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCa                                     >  2:583396/1‑130 (MQ=255)                                                                                                      caccaaccccaaTAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATCCCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGc                         <  2:1035593/140‑1 (MQ=255)                                                                                                       accaacaccaaTAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGCg                        >  1:9438/1‑140 (MQ=255)                                                                                                           acaccaaTAACGAAACCGCAAAGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGCGGTCg                    >  1:508994/1‑140 (MQ=255)                                                                                                            caccaaTAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATCCCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGCGGTCGa                   <  2:36883/140‑1 (MQ=255)                                                                                                            caccaaTAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCa                                     >  2:791271/1‑122 (MQ=255)                                                                                                            caccaaTAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCa                                     <  1:791271/122‑1 (MQ=255)                                                                                                            caccaaTAACGAAACCGCAAGGTTCACCTTTCCCTGAAGAACAACGGGATTTTGTCCTTTACGCCAGACGATGCTGACAAAATCCCCCAGCGGCCACACCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGCGGTCGa                   <  2:717490/140‑1 (MQ=255)                                                                                                             accaaTAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGCGGTCGAc                  >  1:609397/1‑140 (MQ=255)                                                                                                               caaTAACGAAACCGCAAGGTTCACTTTTCCCAAAAGACCACCGGGTTTTTGCCCTTTACGCCAGACGATGCTGACAAAATCCCCCAGCGACCACCCCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGCGGTCGACAg                <  2:628119/140‑1 (MQ=255)                                                                                                                 aTAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCGCCGCCAGCACGCCCTGCAAGCGGTCGACAGTc              <  2:202510/140‑1 (MQ=255)                                                                                                                 aTAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGCGGTCGACAGTc              >  2:486366/1‑140 (MQ=255)                                                                                                                 aTAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGCGGTCGACAGTc              >  1:202510/1‑140 (MQ=255)                                                                                                                  tAACGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGCGGTCGACAGTcc             >  2:921205/1‑140 (MQ=255)                                                                                                                   aaCGAAACCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGCGGTCGACAGTCCa            <  1:717332/140‑1 (MQ=255)                                                                                                                       aaaCCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGCGGTCGACAGTCCAGCCg        >  1:544458/1‑140 (MQ=255)                                                                                                                       aaaCCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGCGGTCGACAGTCCAGCCg        >  2:949359/1‑140 (MQ=255)                                                                                                                        aaCCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGCGGTCGACAGTCCAGCCGt       >  2:176539/1‑140 (MQ=255)                                                                                                                         aCCGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGCGGTCGACAGTCCAGCCGTa      >  2:602347/1‑140 (MQ=255)                                                                                                                          ccGCAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGCGGTCGACAGTCCAGCCGTAc     <  1:318059/140‑1 (MQ=255)                                                                                                                             cAAGGTTCACTTTTCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGCGGTCGACAGTCCAGCCGTACTGa  >  1:187902/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                               ACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCACGCTAATGCGCATACTGTCCAGCACCGGCGAATTAAGAAGCACCAGTATCACCAACACCAATAACGAAACCGCAAGGTTCACTTTGCCCTGAAGAACAACGGGATTTTGCCCTTTACGCCAGACGATGCTGACAAAATACCCCAGCGACCACACCAGTAACACCAGCACCGCCAGCACGCCCTGCAAGCGGTCGACAGTCCAGCCGTACTGA  >  NC_000913/3531250‑3531513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑