Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,579,974 C→A E278* (GAG→TAG)  yhhX ← putative oxidoreductase YhhX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,579,9740CA96.5% 193.4 / ‑0.4 58E278* (GAG→TAG) yhhXputative oxidoreductase YhhX
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (21/35);  total (22/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

AAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTACCGTGAACGATAAATTTCGGATA  >  NC_000913/3579837‑3580109
                                                                                                                                         |                                                                                                                                       
aaGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACa                                                                                                                                       <  1:874402/140‑1 (MQ=255)
     cTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGcaca                                                                                                                                  >  2:655035/1‑140 (MQ=255)
        cAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACaccg                                                                                                                               >  2:743676/1‑140 (MQ=255)
        cAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACaccg                                                                                                                               >  1:829160/1‑140 (MQ=255)
               cTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGaac                                                                                                                        >  2:719207/1‑139 (MQ=255)
                ttGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAatc                                                                                                                       >  1:154991/1‑140 (MQ=255)
                ttGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAatc                                                                                                                       <  2:477591/140‑1 (MQ=255)
                ttGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAatc                                                                                                                       >  2:700013/1‑140 (MQ=255)
                    cGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATcc                                                                                                                   <  1:406487/140‑1 (MQ=255)
                       aaTTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCt                                                                                                                >  2:542881/1‑140 (MQ=255)
                              cgcACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATc                                                                                                         >  2:709177/1‑140 (MQ=255)
                               gcACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCATGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATcc                                                                                                        >  2:781713/1‑140 (MQ=255)
                                   cGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGt                                                                                                    >  1:478315/1‑140 (MQ=255)
                                     tGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGc                                                                                                                 <  1:573360/125‑1 (MQ=255)
                                     tGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGc                                                                                                                 >  2:573360/1‑125 (MQ=255)
                                       ggTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGc                                                                                                <  2:513609/140‑1 (MQ=255)
                                       ggTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGc                                                                                                >  2:16158/1‑140 (MQ=255)
                                        gTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGcc                                                                                               <  1:910416/140‑1 (MQ=255)
                                        gTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGcc                                                                                               <  2:1010848/140‑1 (MQ=255)
                                        gTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGcc                                                                                               <  2:401486/140‑1 (MQ=255)
                                        gTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGcc                                                                                               <  1:759840/140‑1 (MQ=255)
                                        gTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGcc                                                                                               <  1:743676/140‑1 (MQ=255)
                                        gTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGcc                                                                                               <  2:606555/140‑1 (MQ=255)
                                        gTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGcc                                                                                               <  1:557712/140‑1 (MQ=255)
                                         tGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGccc                                                                                              <  1:193507/140‑1 (MQ=255)
                                             ggTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCATGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCa                                                                                          <  1:781713/140‑1 (MQ=255)
                                             ggTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCa                                                                                          <  1:719207/140‑1 (MQ=255)
                                             ggTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCa                                                                                          <  2:578252/140‑1 (MQ=255)
                                             ggTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCa                                                                                          <  1:351390/140‑1 (MQ=255)
                                                ttGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGcc                                                                                               <  2:972208/132‑1 (MQ=255)
                                                ttGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGcc                                                                                               >  1:972208/1‑132 (MQ=255)
                                                ttGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCataa                                                                                       <  2:829160/140‑1 (MQ=255)
                                                 tGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCataat                                                                                      >  2:73044/1‑140 (MQ=255)
                                                           catcatAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTc                                                                            >  2:861920/1‑140 (MQ=255)
                                                               atAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGc                                                                        <  1:709177/140‑1 (MQ=255)
                                                                    cgcgCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGtt                                                                   <  1:542881/140‑1 (MQ=255)
                                                                       gcCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTcc                                                                <  2:715851/140‑1 (MQ=255)
                                                                        cccGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGc                                                                        <  2:340318/131‑1 (MQ=255)
                                                                        cccGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGc                                                                        >  1:340318/1‑131 (MQ=255)
                                                                        cccGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCct                                                               >  2:1012342/1‑140 (MQ=255)
                                                                        cccGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCct                                                               >  2:387230/1‑140 (MQ=255)
                                                                         ccGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCctg                                                              <  2:1009236/140‑1 (MQ=255)
                                                                         ccGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCctg                                                              <  2:300102/140‑1 (MQ=255)
                                                                         ccGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGACCGGCATAATATTAGCCTTTAGGCTGGTTTCctg                                                              <  1:700013/140‑1 (MQ=255)
                                                                              aTCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGt                                                         <  1:1012342/140‑1 (MQ=255)
                                                                                 gCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGa                                                      <  1:769829/140‑1 (MQ=255)
                                                                                   ccATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATa                                                    <  1:583756/140‑1 (MQ=255)
                                                                                            gCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTta                                           <  1:115411/140‑1 (MQ=255)
                                                                                            gCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTta                                           >  2:709566/1‑140 (MQ=255)
                                                                                                cATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTtaataa                                       <  2:154991/140‑1 (MQ=255)
                                                                                                 aTCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATaaa                                      <  1:226610/140‑1 (MQ=255)
                                                                                                 aTCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATaaa                                      >  2:391850/1‑140 (MQ=255)
                                                                                                    tcttctctGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGa                                   <  2:204510/140‑1 (MQ=255)
                                                                                                      ttctctGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAAc                                 <  1:387230/140‑1 (MQ=255)
                                                                                                      ttctctGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAAc                                 <  1:16158/140‑1 (MQ=255)
                                                                                                      ttctctGACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAAc                                 <  1:655035/140‑1 (MQ=255)
                                                                                                            gACCGTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCt                           >  2:905674/1‑140 (MQ=255)
                                                                                                                gTCACGCCCTCGTCATTGACATACTACAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTAcc                       >  1:634333/1‑140 (MQ=255)
                                                                                                                                     tactacAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTACCGTGAACGATAAATTTCGGATa  >  2:330016/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
AAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTACCGTGAACGATAAATTTCGGATA  >  NC_000913/3579837‑3580109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑