Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	180,422	G→A	V396I (GTC→ATC)	dgt →	dGTP triphosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	180,422	0	G	A	100.0%	318.9 / NA	88	V396I (GTC→ATC)	dgt	dGTP triphosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (49/39);  total (49/39)

CGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGAAAAAGAACGGGTGAAACGTTTCCCTATTGAA  >  NC_000913/180287‑180556                                                                                                                                        |                                                                                                                                       cGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGa                                                                                                                                    >  2:499747/1‑140 (MQ=255)      tATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGc                                                                                                                               >  2:853696/1‑140 (MQ=255)      tATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGc                                                                                                                               <  2:1049147/140‑1 (MQ=255)      tATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGc                                                                                                                               >  2:915770/1‑140 (MQ=255)             aaTCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAAtt                                                                                                                        >  2:685000/1‑140 (MQ=255)             aaTCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAAtt                                                                                                                        >  1:900646/1‑140 (MQ=255)             aaTCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAAtt                                                                                                                        >  2:543289/1‑140 (MQ=255)                  gcctgcGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGa                                                                                                                                    >  2:776287/1‑123 (MQ=255)                  gcctgcGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGa                                                                                                                                    <  1:776287/123‑1 (MQ=255)                     tgcGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCa                                                                                                                     >  2:62592/1‑135 (MQ=255)                     tgcGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCa                                                                                                                     <  1:62592/135‑1 (MQ=255)                     tgcGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCt                                                                                                                <  2:728621/140‑1 (MQ=255)                       cGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTAt                                                                                                              <  1:979652/140‑1 (MQ=255)                            ttCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGt                                                                                                         <  2:161021/140‑1 (MQ=255)                                    aaCGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCg                                                                                                 <  2:1167624/140‑1 (MQ=255)                                                 gCATTATTGGAAGATGCCAGCGAATGCAGCGAACTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGTCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGAt                                                                                    >  1:136417/1‑140 (MQ=255)                                                  cATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCg                                                                                                 <  2:571037/126‑1 (MQ=255)                                                  cATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGCCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCACGGCTATCGGGTCATTAGCg                                                                                                 >  1:571037/1‑126 (MQ=255)                                                    ttattGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTa                                                                                 <  1:853696/140‑1 (MQ=255)                                                     tattGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTAt                                                                                >  2:548905/1‑140 (MQ=255)                                                       ttGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCg                                                                              >  1:831384/1‑140 (MQ=255)                                                         ggAAGATGCCAGCGAATGCAGCGAACTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGTCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTc                                                                            <  2:136417/140‑1 (MQ=255)                                                          gAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTcc                                                                           <  1:74516/140‑1 (MQ=255)                                                          gAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTcc                                                                           <  2:864481/140‑1 (MQ=255)                                                            aGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCtt                                                                         >  2:1097336/1‑140 (MQ=255)                                                            aGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCtt                                                                         >  2:280571/1‑140 (MQ=255)                                                               tGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCGGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTttat                                                                      >  2:390185/1‑140 (MQ=255)                                                               tGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTcc                                                                           >  2:936927/1‑135 (MQ=255)                                                               tGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTcc                                                                           <  2:828986/135‑1 (MQ=255)                                                               tGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTcc                                                                           >  1:828986/1‑135 (MQ=255)                                                               tGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTcc                                                                           <  1:936927/135‑1 (MQ=255)                                                               tGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTttat                                                                      >  2:92236/1‑140 (MQ=255)                                                               tGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTttat                                                                      >  1:171113/1‑140 (MQ=255)                                                               tGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTttat                                                                      >  1:448362/1‑140 (MQ=255)                                                               tGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTttat                                                                      >  1:770599/1‑140 (MQ=255)                                                               tGCCAGCGAATGCAGCGATCTTCTTAAGCTACATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTttat                                                                      >  2:902713/1‑140 (MQ=255)                                                                     cGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGcc                                                                >  2:435028/1‑140 (MQ=255)                                                                     cGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGcc                                                                >  2:79043/1‑140 (MQ=255)                                                                        aTGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGt                                                             <  1:311863/140‑1 (MQ=255)                                                                         tGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTc                                                            >  2:275052/1‑140 (MQ=255)                                                                         tGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTc                                                            <  1:685000/140‑1 (MQ=255)                                                                              cGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTAt                                                       >  1:778176/1‑140 (MQ=255)                                                                               gATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATc                                                      <  1:915770/140‑1 (MQ=255)                                                                               gATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATc                                                      <  2:143081/140‑1 (MQ=255)                                                                                aTCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCa                                                     <  2:778176/140‑1 (MQ=255)                                                                                  cttcttAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGa                                                   <  1:1092986/140‑1 (MQ=255)                                                                                  cttcttAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGa                                                   <  2:448362/140‑1 (MQ=255)                                                                                  cttcttAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGa                                                   <  1:435028/140‑1 (MQ=255)                                                                                  cttcttAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGa                                                   <  1:543289/140‑1 (MQ=255)                                                                                  cttcttAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGa                                                   <  1:851029/140‑1 (MQ=255)                                                                                  cttcttAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGa                                                   <  1:79043/140‑1 (MQ=255)                                                                                  cttcttAAGCTACATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGa                                                   <  1:902713/140‑1 (MQ=255)                                                                                        aaGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTAc                                             <  1:499747/140‑1 (MQ=255)                                                                                        aaGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTAc                                             <  2:900646/140‑1 (MQ=255)                                                                                          gCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTg                                           <  1:129069/140‑1 (MQ=255)                                                                                           cTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGa                                          >  1:80102/1‑140 (MQ=255)                                                                                             atataAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAAc                                        >  2:901116/1‑140 (MQ=255)                                                                                             atataAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAAc                                        >  2:439992/1‑140 (MQ=255)                                                                                              tataAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACt                                       <  2:831384/140‑1 (MQ=255)                                                                                              tataAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACt                                       >  2:842617/1‑140 (MQ=255)                                                                                              tataAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACt                                       >  1:841125/1‑140 (MQ=255)                                                                                               ataAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGa                                                   >  1:118941/1‑127 (MQ=255)                                                                                               ataAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGa                                                   <  2:118941/127‑1 (MQ=255)                                                                                               ataAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACt                                       >  1:781023/1‑139 (MQ=255)                                                                                               ataAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACt                                       <  2:781023/139‑1 (MQ=255)                                                                                                     aTGTCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGaa                                >  2:576410/1‑140 (MQ=255)                                                                                                        tCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGaaaaa                             >  2:970193/1‑140 (MQ=255)                                                                                                        tCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGaaaaa                             >  2:29244/1‑140 (MQ=255)                                                                                                        tCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGaaaaa                             >  2:113207/1‑140 (MQ=255)                                                                                                        tCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGaaaaa                             >  1:972337/1‑140 (MQ=255)                                                                                                        tCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGaaaaa                             >  1:611708/1‑140 (MQ=255)                                                                                                        tCGCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGaaaaa                             >  1:598527/1‑140 (MQ=255)                                                                                                          gCTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGAAAAAGa                           >  1:570378/1‑140 (MQ=255)                                                                                                           cTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGAAAAAGaa                          <  1:548905/140‑1 (MQ=255)                                                                                                           cTGTAAAACATGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGAAAAAGaa                          <  1:588332/140‑1 (MQ=255)                                                                                                                    aTGTGTTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGAAAAAGAACGGGTGaaa                 <  2:659504/140‑1 (MQ=255)                                                                                                                        gtTTAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGAAAAAGAACGGGTGAAACGtt             >  2:235676/1‑140 (MQ=255)                                                                                                                         tttAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGAAAAAGAACGGGTGAAACGttt            <  1:113207/140‑1 (MQ=255)                                                                                                                         tttAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGAAAAAGAACGGGTGAAACGttt            <  1:199427/140‑1 (MQ=255)                                                                                                                         tttAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGAAAAAGAACGGGTGAAACGttt            <  1:280571/140‑1 (MQ=255)                                                                                                                         tttAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGAAAAAGAACGGGTGAAACGttt            >  2:155121/1‑140 (MQ=255)                                                                                                                          ttAGCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTAATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGAAAAAGAACGGGTGAAACGTTTc           >  2:74543/1‑140 (MQ=255)                                                                                                                             gCCATCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGAAAAAGAACGGGTGAAACGTTTCCCt        >  1:628989/1‑140 (MQ=255)                                                                                                                                 tCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGAAAAAGAACGGGTGAAACGTTTCCCTATTg    >  2:8648/1‑140 (MQ=255)                                                                                                                                 tCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGAAAAAGAACGGGTGAAACGTTTCCCTATTg    >  1:354658/1‑140 (MQ=255)                                                                                                                                 tCCAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTAAACTGGTAGAAAAAGAACGGGTGAAACGTTTCCCTATTg    >  1:1135567/1‑140 (MQ=255)                                                                                                                                   cAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGAAAAAGAACGGGTGAAACGTTTCCCTATTGaa  <  1:128230/140‑1 (MQ=255)                                                                                                                                   cAGATATCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGAAAAAGAACGGGTGAAACGTTTCCCTATTGaa  >  2:637296/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       CGATTTATTGATAATCTGCCTGCGATTTTCGCCGGAACGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTCGAGCGGCTTGAATTGCAGGGCTATCGGGTCATTAGCGGATTATTAGAGATTTATCGTCCTTTATTAAGCCTGTCGTTATCAGACTTTACTGAACTGGTAGAAAAAGAACGGGTGAAACGTTTCCCTATTGAA  >  NC_000913/180287‑180556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑