Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,225,335	C→A	S9S (TCG→TCT)	minD ←	Z‑ring positioning protein MinD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,225,335	0	C	A	100.0%	203.3 / NA	57	S9S (TCG→TCT)	minD	Z‑ring positioning protein MinD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/30);  total (27/30)

CGTTCACAACCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCAC  >  NC_000913/1225201‑1225470                                                                                                                                       |                                                                                                                                        cGTTCACAACCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGt                                                                                                                                    <  1:166731/140‑1 (MQ=255)   ttCACAACCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTaa                                                                                                                                  <  1:154691/140‑1 (MQ=255)    tCACAACCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTaac                                                                                                                                 <  2:387988/140‑1 (MQ=255)      acaACCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTaacaa                                                                                                                               >  1:82599/1‑140 (MQ=255)      acaACCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTaacaa                                                                                                                               <  2:575510/140‑1 (MQ=255)           ccATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTaac                                                                                                                                 <  1:739088/133‑1 (MQ=255)           ccATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTaac                                                                                                                                 >  2:739088/1‑133 (MQ=255)                aTCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCg                                                                                                                     <  2:445708/140‑1 (MQ=255)                  cAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTg                                                                                                                   >  1:733907/1‑140 (MQ=255)                   aGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGc                                                                                                                  <  2:795969/140‑1 (MQ=255)                   aGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGc                                                                                                                  <  2:68087/140‑1 (MQ=255)                    ggTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGcc                                                                                                                 >  2:981440/1‑140 (MQ=255)                    ggTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGCTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGcc                                                                                                                 >  1:99725/1‑140 (MQ=255)                     gTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAAcaacaa                                                                                                                            <  2:598966/128‑1 (MQ=255)                     gTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAAcaacaa                                                                                                                            >  1:598966/1‑128 (MQ=255)                     gTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCg                                                                                                                     >  2:1078369/1‑135 (MQ=255)                     gTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCg                                                                                                                     <  2:670844/135‑1 (MQ=255)                     gTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCg                                                                                                                     >  2:106818/1‑135 (MQ=255)                     gTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCg                                                                                                                     >  1:670844/1‑135 (MQ=255)                     gTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCg                                                                                                                     <  1:1078369/135‑1 (MQ=255)                     gTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCg                                                                                                                     <  1:106818/135‑1 (MQ=255)                     gTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCa                                                                                                                >  1:1020944/1‑140 (MQ=255)                     gTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCa                                                                                                                >  2:154656/1‑140 (MQ=255)                     gTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCa                                                                                                                >  2:198899/1‑140 (MQ=255)                               cGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCTCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTcc                                                                                                      <  2:976663/140‑1 (MQ=255)                               cGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTcc                                                                                                      <  1:31702/140‑1 (MQ=255)                               cGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCCGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTcc                                                                                                      >  1:1067576/1‑140 (MQ=255)                                   ggCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGt                                                                                                  >  1:348772/1‑140 (MQ=255)                                         tatCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTaaaaa                                                                                            <  2:99725/140‑1 (MQ=255)                                              aaaTCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAggg                                                                                         >  1:242674/1‑138 (MQ=255)                                              aaaTCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAggg                                                                                         <  2:242674/138‑1 (MQ=255)                                              aaaTCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGAt                                                                                       <  1:981440/140‑1 (MQ=255)                                              aaaTCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGAt                                                                                       <  1:984263/140‑1 (MQ=255)                                                  cTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAAt                                                                                   >  2:1122787/1‑140 (MQ=255)                                                     tCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAggg                                                                                         >  2:1112746/1‑131 (MQ=255)                                                     tCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAggg                                                                                         <  1:1112746/131‑1 (MQ=255)                                                     tCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTa                                                                                >  2:27739/1‑140 (MQ=255)                                                              ttttCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGaa                                                                       <  1:27739/140‑1 (MQ=255)                                                              ttttCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGaa                                                                       <  1:291101/140‑1 (MQ=255)                                                                ttctttcCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACg                                                                     >  2:801770/1‑140 (MQ=255)                                                                        cTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGc                                                             >  1:900931/1‑140 (MQ=255)                                                                            tGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGttt                                                         >  2:1132348/1‑140 (MQ=255)                                                                            tGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGttt                                                         <  1:801770/140‑1 (MQ=255)                                                                                 cAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGTGTTTTCGAc                                                    <  2:1132135/140‑1 (MQ=255)                                                                                 cAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGAc                                                    <  1:154656/140‑1 (MQ=255)                                                                                  aaaCCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACt                                                   <  1:793947/140‑1 (MQ=255)                                                                                   aaCCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTa                                                  >  1:19104/1‑140 (MQ=255)                                                                                        gTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGc                                             >  1:548851/1‑140 (MQ=255)                                                                                         tGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCa                                            >  2:274836/1‑140 (MQ=255)                                                                                                ggCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCgcgc                                     >  2:440204/1‑140 (MQ=255)                                                                                                    gcgcTGGAGGTTGTCTTACCAACACCCCATTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCt                                 <  2:993089/140‑1 (MQ=255)                                                                                                                 tCTTACCAACACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAAtt                    >  2:912281/1‑140 (MQ=255)                                                                                                                        aaCACCCCCTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTgg             <  1:1136114/140‑1 (MQ=255)                                                                                                                              cccTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTg       <  1:544554/140‑1 (MQ=255)                                                                                                                               ccTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGa      >  2:161028/1‑140 (MQ=255)                                                                                                                                cTTTGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGAt     <  1:912281/140‑1 (MQ=255)                                                                                                                                   tGCCAGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCAc  <  2:1020944/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        CGTTCACAACCCATAATCAGGTCGAGATTACGCAGGCCGATATCAAAATCTATCACGACAGTTTTCTTTCCCTTCTGGGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTGTCTTACCAACACCCCCTTTGCCCGAAGTAACAACAATAATGCGTGCCATAGAAATTCCTTGTTAAAAAGGGATCAATTTAACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCAC  >  NC_000913/1225201‑1225470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑