Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,608,834	G→T	A63A (GCC→GCA)	yneE ←	conserved inner membrane protein YneE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,608,834	0	G	T	100.0%	303.0 / NA	84	A63A (GCC→GCA)	yneE	conserved inner membrane protein YneE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (39/45);  total (39/45)

TTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAG  >  NC_000913/1608698‑1608969                                                                                                                                         |                                                                                                                                        ttGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCATCGTAACGGGCGTTCCCGGCATTATTACGAAAACCAAGAAAAatggcgattgcg                                                                                                                                      <  1:205843/140‑1 (MQ=255) ttGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAatggcgattgcg                                                                                                                                      <  1:562118/140‑1 (MQ=255)   gCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGac                                                                                                                                    >  1:1058804/1‑140 (MQ=255)   gCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGac                                                                                                                                    >  1:1032563/1‑140 (MQ=255)   gCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGac                                                                                                                                    >  2:774952/1‑140 (MQ=255)   gCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGac                                                                                                                                    >  2:578635/1‑140 (MQ=255)   gCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGac                                                                                                                                    >  1:583109/1‑140 (MQ=255)   gCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGac                                                                                                                                    >  1:345615/1‑140 (MQ=255)   gCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGac                                                                                                                                    >  1:344044/1‑140 (MQ=255)   gCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTA‑TACCAAAACCAAAAAAAATGGCGATTGCGaca                                                                                                                                   >  1:923807/1‑140 (MQ=255)   gCCGAATCCGGCAATGTCGTTTTTACCTAGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGac                                                                                                                                    >  2:985311/1‑140 (MQ=255)   gCCGAATCCGGCAATGTCGCTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACAAAGAAAAATGGCGATTGCGac                                                                                                                                    >  2:743811/1‑140 (MQ=255)        aTCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCga                                                                                                                               <  2:185143/140‑1 (MQ=255)        aTCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCga                                                                                                                               <  1:626468/140‑1 (MQ=255)         tCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCgag                                                                                                                              >  1:880432/1‑140 (MQ=255)                  gTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGaa                                                                                                                     <  1:312832/140‑1 (MQ=255)                  gTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGaa                                                                                                                     <  2:28252/140‑1 (MQ=255)                   tCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAAc                                                                                                                    >  2:975063/1‑140 (MQ=255)                      tttttACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGt                                                                                                                 >  1:578287/1‑140 (MQ=255)                            ccTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAgg                                                                                                           <  1:137297/140‑1 (MQ=255)                                gcgcAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGa                                                                                                       <  2:1084505/140‑1 (MQ=255)                                   cAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAAtt                                                                                                    <  1:1086784/140‑1 (MQ=255)                                     gTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTg                                                                                                  <  2:1058804/140‑1 (MQ=255)                                     gTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTg                                                                                                  <  1:748219/140‑1 (MQ=255)                                           aCCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGccc                                                                                            >  1:959106/1‑140 (MQ=255)                                           aCCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGccc                                                                                            >  1:62143/1‑140 (MQ=255)                                            ccGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCa                                                                                           >  2:925541/1‑140 (MQ=255)                                            ccGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCa                                                                                           <  1:608563/140‑1 (MQ=255)                                             cGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAg                                                                                          <  2:440993/140‑1 (MQ=255)                                             cGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAg                                                                                          <  1:925541/140‑1 (MQ=255)                                              gTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGa                                                                                         >  1:287253/1‑140 (MQ=255)                                                gagaCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATg                                                                                       >  2:853268/1‑140 (MQ=255)                                                gAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTg                                                                                                  >  2:387758/1‑129 (MQ=255)                                                gAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTg                                                                                                  <  1:387758/129‑1 (MQ=255)                                                gAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATg                                                                                       >  1:309366/1‑140 (MQ=255)                                                  ggCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCg                                                                                     <  2:62143/140‑1 (MQ=255)                                                  ggCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCg                                                                                     <  1:774952/140‑1 (MQ=255)                                                  ggCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCg                                                                                     <  1:555654/140‑1 (MQ=255)                                                    cAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCgtg                                                                                   <  2:1032563/140‑1 (MQ=255)                                                    cAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCgtg                                                                                   >  1:1086510/1‑140 (MQ=255)                                                      atcatcAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTa                                                                                 <  1:975063/140‑1 (MQ=255)                                                      atcatcAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTa                                                                                 >  2:534488/1‑140 (MQ=255)                                                      atcatcAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTa                                                                                 >  2:45269/1‑140 (MQ=255)                                                             aCTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgc                                                                          >  2:568032/1‑140 (MQ=255)                                                              cTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgca                                                                         >  2:1032446/1‑140 (MQ=255)                                                                gCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgcagc                                                                       <  2:880432/140‑1 (MQ=255)                                                                 cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgcagca                                                                      >  1:853723/1‑140 (MQ=255)                                                                 cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgcagca                                                                      >  2:838631/1‑140 (MQ=255)                                                                 cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgcagca                                                                      >  1:12151/1‑140 (MQ=255)                                                                 cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgcagca                                                                      >  2:333003/1‑140 (MQ=255)                                                                 cccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCG‑GGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCAt                                                                     >  2:688499/1‑140 (MQ=255)                                                                  ccccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCAt                                                                     <  1:333003/140‑1 (MQ=255)                                                                   cccAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATg                                                                    >  1:209012/1‑140 (MQ=255)                                                                      aaaGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGaaa                                                                 >  1:754473/1‑140 (MQ=255)                                                                           tttCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAAc                                                            <  2:959106/140‑1 (MQ=255)                                                                                  cTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATa                                                     >  2:627439/1‑140 (MQ=255)                                                                                    tCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGa                                                   >  1:628956/1‑140 (MQ=255)                                                                                    tCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGa                                                   <  1:633398/140‑1 (MQ=255)                                                                                      aaCGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGaaa                                                 <  2:345615/140‑1 (MQ=255)                                                                                         gTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAaga                                              <  1:936297/140‑1 (MQ=255)                                                                                          tAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCG‑GGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAgaga                                            <  1:688499/140‑1 (MQ=255)                                                                                           aaCGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAgaga                                            <  2:301420/140‑1 (MQ=255)                                                                                           aaCGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAgaga                                            <  1:45269/140‑1 (MQ=255)                                                                                                 cGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGTGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTg                                      >  2:687888/1‑140 (MQ=255)                                                                                                 cGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTg                                      <  2:688393/140‑1 (MQ=255)                                                                                                   tACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGag                                    >  2:813436/1‑140 (MQ=255)                                                                                                    aCCCGGCATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGagt                                   >  1:516529/1‑140 (MQ=255)                                                                                                          cATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAg                             <  1:578635/140‑1 (MQ=255)                                                                                                          cATTATTACGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAg                             >  2:575307/1‑140 (MQ=255)                                                                                                            ttattaCGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAgcg                           <  2:12151/140‑1 (MQ=255)                                                                                                             tattaCGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAgcgc                          <  2:853723/140‑1 (MQ=255)                                                                                                                taCGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAg                       >  1:681715/1‑140 (MQ=255)                                                                                                                 aCGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAgg                      <  1:813436/140‑1 (MQ=255)                                                                                                                  cGAAAACCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGa                     <  2:583109/140‑1 (MQ=255)                                                                                                                      aaCCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGTGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATc                 <  1:687888/140‑1 (MQ=255)                                                                                                                      aaCCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATc                 <  2:344044/140‑1 (MQ=255)                                                                                                                      aaCCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATc                 <  1:710853/140‑1 (MQ=255)                                                                                                                      aaCCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATc                 <  2:209012/140‑1 (MQ=255)                                                                                                                      aaCCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATc                 <  1:853268/140‑1 (MQ=255)                                                                                                                      aaCCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATc                 <  2:447795/140‑1 (MQ=255)                                                                                                                      aaCCAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATc                 <  2:628956/140‑1 (MQ=255)                                                                                                                         cAAGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTg              >  2:54675/1‑140 (MQ=255)                                                                                                                           aGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            <  1:743811/140‑1 (MQ=255)                                                                                                                           aGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            <  2:313090/140‑1 (MQ=255)                                                                                                                           aGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            <  2:516529/140‑1 (MQ=255)                                                                                                                           aGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            <  2:287253/140‑1 (MQ=255)                                                                                                                           aGAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGa            <  1:838631/140‑1 (MQ=255)                                                                                                                            gAAAAATGGCGATTGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGat           >  2:1142870/1‑140 (MQ=255)                                                                                                                                     cgattgcgaCACCGAGAATGCTGAACGGTGCGAGGGCGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAg  >  1:871614/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAG  >  NC_000913/1608698‑1608969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑