Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,931,446	C→A	A189E (GCG→GAG)	purT →	phosphoribosylglycinamide formyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,931,446	0	C	A	100.0%	256.3 / NA	71	A189E (GCG→GAG)	purT	phosphoribosylglycinamide formyltransferase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (36/35);  total (36/35)

CGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATCC  >  NC_000913/1931311‑1931579                                                                                                                                        |                                                                                                                                      cGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGc                                                                                                                                   <  2:468373/140‑1 (MQ=255)   gTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCcg                                                                                                                                 <  2:976190/140‑1 (MQ=255)     tGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCcgcg                                                                                                                               <  2:613806/140‑1 (MQ=255)      gCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGt                                                                                                                              <  1:958719/140‑1 (MQ=255)                 cTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGc                                                                                                                                   >  1:1043962/1‑124 (MQ=255)                 cTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGc                                                                                                                                   <  2:1043962/124‑1 (MQ=255)                 cTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAg                                                                                                                   >  1:193909/1‑140 (MQ=255)                  tATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAgg                                                                                                                  <  2:767619/140‑1 (MQ=255)                   aTCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGc                                                                                                                 <  2:257189/140‑1 (MQ=255)                    tCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGcg                                                                                                                <  2:452801/140‑1 (MQ=255)                    tCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGcg                                                                                                                <  2:355874/140‑1 (MQ=255)                    tCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGcg                                                                                                                >  2:835328/1‑140 (MQ=255)                      ccTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGcgtc                                                                                                              >  2:951208/1‑140 (MQ=255)                       cTGCATTGTAAAACCGGTGATGGGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGcgtcg                                                                                                             >  2:121469/1‑140 (MQ=255)                          cATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTa                                                                                                          >  1:1022962/1‑140 (MQ=255)                          cATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTa                                                                                                          >  2:346894/1‑140 (MQ=255)                          cATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTa                                                                                                          >  1:790200/1‑140 (MQ=255)                               tAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGttt                                                                                                     >  2:591454/1‑140 (MQ=255)                               tAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGtt                                                                                                      >  2:491435/1‑139 (MQ=255)                               tAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGtt                                                                                                      <  1:491435/139‑1 (MQ=255)                                  aaCCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGAc                                                                                                  <  1:340478/140‑1 (MQ=255)                                    ccGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACtt                                                                                                >  1:22428/1‑140 (MQ=255)                                      ggTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACtt                                                                                                <  2:344772/138‑1 (MQ=255)                                      ggTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACtt                                                                                                >  1:344772/1‑138 (MQ=255)                                       gTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGa                                                                                             <  1:951208/140‑1 (MQ=255)                                        tgatgaGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGaa                                                                                            >  2:973122/1‑140 (MQ=255)                                         gatgaGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGaaa                                                                                           <  1:936032/140‑1 (MQ=255)                                         gatgaGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGaaa                                                                                           <  2:812093/140‑1 (MQ=255)                                                    ccGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTa                                                                                <  2:1022962/140‑1 (MQ=255)                                                    ccGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTa                                                                                >  1:277650/1‑140 (MQ=255)                                                            gggCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAg                                                                        <  1:121469/140‑1 (MQ=255)                                                            gggCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAg                                                                        >  2:47899/1‑140 (MQ=255)                                                            gggCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAg                                                                        >  2:947732/1‑140 (MQ=255)                                                                aGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGcgcg                                                                    >  1:584921/1‑140 (MQ=255)                                                                  aCGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGt                                                                  <  2:917516/140‑1 (MQ=255)                                                                    gTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTgg                                                                <  2:221009/140‑1 (MQ=255)                                                                       tATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATg                                                             <  1:135965/140‑1 (MQ=255)                                                                       tATTCGTTCTGCAGAGCAACTTGCACAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATg                                                             >  2:1142862/1‑140 (MQ=255)                                                                            gTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTc                                                        >  2:741661/1‑140 (MQ=255)                                                                               cTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCAt                                                     >  1:1080925/1‑140 (MQ=255)                                                                                 gCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCAttt                                                   <  1:57139/140‑1 (MQ=255)                                                                                      gCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCtgtg                                              <  1:947732/140‑1 (MQ=255)                                                                                       cAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGc                                             <  1:973122/140‑1 (MQ=255)                                                                                          cTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCtgtg                                              <  2:647501/136‑1 (MQ=255)                                                                                          cTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCtgtg                                              >  1:647501/1‑136 (MQ=255)                                                                                          cTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCtgt                                               >  2:718941/1‑135 (MQ=255)                                                                                          cTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCtgt                                               <  1:718941/135‑1 (MQ=255)                                                                                               tCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTc                                                  <  2:828424/127‑1 (MQ=255)                                                                                               tCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTc                                                  >  1:828424/1‑127 (MQ=255)                                                                                                 aGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGt                                   <  1:272268/140‑1 (MQ=255)                                                                                                   gCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCa                                 <  2:290054/140‑1 (MQ=255)                                                                                                   gCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGGCa                                 <  2:727161/140‑1 (MQ=255)                                                                                                    cATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCAt                                >  1:1086896/1‑140 (MQ=255)                                                                                                        gAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGcc                            <  2:193909/140‑1 (MQ=255)                                                                                                        gAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGcc                            >  2:25877/1‑140 (MQ=255)                                                                                                             aCGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGaa                       >  1:860827/1‑140 (MQ=255)                                                                                                             aCGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGaa                       >  2:972228/1‑140 (MQ=255)                                                                                                             aCGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGaa                       >  2:699155/1‑140 (MQ=255)                                                                                                              cGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAg                      >  1:349227/1‑140 (MQ=255)                                                                                                              cGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAg                      <  2:14383/140‑1 (MQ=255)                                                                                                              cGCTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAg                      >  2:169228/1‑140 (MQ=255)                                                                                                                cTCAGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGAt                    <  2:721256/140‑1 (MQ=255)                                                                                                                   aGCAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGc                 >  1:181691/1‑140 (MQ=255)                                                                                                                     cAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGCGa               <  2:22428/140‑1 (MQ=255)                                                                                                                     cAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGCGa               <  2:755042/140‑1 (MQ=255)                                                                                                                     cAAGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGCGa               <  2:790200/140‑1 (MQ=255)                                                                                                                      aaGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGc                 >  1:453702/1‑137 (MQ=255)                                                                                                                      aaGGCGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGc                 <  2:453702/137‑1 (MQ=255)                                                                                                                          cGGTCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGCGACTAcc          >  1:1010597/1‑140 (MQ=255)                                                                                                                             tCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGCGACTACCGTg       >  1:909434/1‑140 (MQ=255)                                                                                                                             tCGCGCCGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGCGACTACCGTg       >  1:588953/1‑140 (MQ=255)                                                                                                                                  ccGGAGAGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATcc  >  1:581765/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      CGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAACCGTCAGCGCGGTGGATGGCGTCCATTTCTGTGCACCAGTAGGTCATCGCCAGGAAGATGGCGACTACCGTGAATCC  >  NC_000913/1931311‑1931579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑