Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,339,278	A→T	V48E (GTA→GAA)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,339,278	0	A	T	98.7%	278.4 / ‑4.0	79	V48E (GTA→GAA)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (46/32);  total (46/33)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

GCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGG  >  NC_000913/2339141‑2339412                                                                                                                                          |                                                                                                                                       gcgcCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTcg                                                                                                                                      <  1:1051731/140‑1 (MQ=255)  cgcCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTcgc                                                                                                                                     <  2:868513/140‑1 (MQ=255)  cgcCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTcgc                                                                                                                                     <  1:931127/140‑1 (MQ=255)    ccATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGa                                                                                                                                   <  1:71161/140‑1 (MQ=255)    ccATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGa                                                                                                                                   <  1:544653/140‑1 (MQ=255)       tGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACgg                                                                                                                                >  2:758784/1‑140 (MQ=255)       tGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACgg                                                                                                                                >  2:231674/1‑140 (MQ=255)       tGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACgg                                                                                                                                >  1:251864/1‑140 (MQ=255)            aCGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTAc                                                                                                                           >  1:729306/1‑140 (MQ=255)             cGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTAcc                                                                                                                          <  2:519869/140‑1 (MQ=255)               aTCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACAAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCgg                                                                                                                        <  1:748165/140‑1 (MQ=255)                 cGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTAc                                                                                                                           >  2:1024126/1‑135 (MQ=255)                 cGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTAc                                                                                                                           <  1:1024126/135‑1 (MQ=255)                   tgtCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTc                                                                                                                    >  2:880130/1‑140 (MQ=255)                   tgtCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTc                                                                                                                    >  2:1116071/1‑140 (MQ=255)                       aTAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGc                                                                                                                <  1:857678/140‑1 (MQ=255)                       aTAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGc                                                                                                                <  2:660485/140‑1 (MQ=255)                        tAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGcc                                                                                                               >  1:255368/1‑140 (MQ=255)                        tAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGcc                                                                                                               >  1:211020/1‑140 (MQ=255)                          gACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCAt                                                                                                             >  2:158625/1‑140 (MQ=255)                               ccgAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCgg                                                                                                        >  2:40802/1‑140 (MQ=255)                                  aGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACa                                                                                                     >  2:1142958/1‑140 (MQ=255)                                             ggATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAgcgc                                                                                          <  1:667344/140‑1 (MQ=255)                                              gATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCa                                                                                         <  2:729306/140‑1 (MQ=255)                                              gATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCa                                                                                         <  1:679996/140‑1 (MQ=255)                                              gATGGTATTTACCGATTACGTCACCAACGACACAGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCa                                                                                         >  1:697678/1‑140 (MQ=255)                                                tGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACg                                                                                       >  1:859292/1‑140 (MQ=255)                                                  gTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGc                                                                                     >  2:320927/1‑140 (MQ=255)                                                  gTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGc                                                                                     >  2:732766/1‑140 (MQ=255)                                                       tACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCcaaca                                                                                >  1:443498/1‑140 (MQ=255)                                                       tACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCcaaca                                                                                >  1:996481/1‑140 (MQ=255)                                                        aCCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCcaacaa                                                                               >  2:806164/1‑140 (MQ=255)                                                        aCCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCcaacaa                                                                               >  1:448803/1‑140 (MQ=255)                                                            aTTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGAc                                                                           <  1:806164/140‑1 (MQ=255)                                                            aTTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGAc                                                                           >  1:798249/1‑140 (MQ=255)                                                             ttACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGAcc                                                                          >  2:805768/1‑140 (MQ=255)                                                             ttACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGAcc                                                                          >  1:1154586/1‑140 (MQ=255)                                                             ttACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGAcc                                                                          >  1:976228/1‑140 (MQ=255)                                                             ttACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGAcc                                                                          >  2:822597/1‑140 (MQ=255)                                                             ttACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGAcc                                                                          >  2:178203/1‑140 (MQ=255)                                                             ttACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGAcc                                                                          >  2:831477/1‑140 (MQ=255)                                                             ttACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGAcc                                                                          >  2:1108665/1‑140 (MQ=255)                                                                  tCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACAt                                                                     >  2:290855/1‑140 (MQ=255)                                                                    aCCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCg                                                                   >  1:684943/1‑140 (MQ=255)                                                                      cAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCa                                                                 <  2:497177/140‑1 (MQ=255)                                                                      cAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCa                                                                 <  1:1116071/140‑1 (MQ=255)                                                                      cAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCa                                                                 <  2:996481/140‑1 (MQ=255)                                                                      cAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCa                                                                 <  1:320927/140‑1 (MQ=255)                                                                      cAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCa                                                                 <  1:231674/140‑1 (MQ=255)                                                                       aaCGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCAt                                                                <  2:448803/140‑1 (MQ=255)                                                                        aCGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATa                                                               >  2:994473/1‑140 (MQ=255)                                                                          gACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAAt                                                             >  1:1067617/1‑140 (MQ=255)                                                                           acacGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATc                                                            <  2:251864/140‑1 (MQ=255)                                                                           acacGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATc                                                            <  1:1142958/140‑1 (MQ=255)                                                                                   aGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAgg                                                    <  1:880130/140‑1 (MQ=255)                                                                                           tataGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGagctcttc                                            >  2:824938/1‑140 (MQ=255)                                                                                               ggCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGagctcttcagct                                        <  2:211020/140‑1 (MQ=255)                                                                                               ggCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGagctcttcagct                                        >  1:755352/1‑140 (MQ=255)                                                                                                 cTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGagctcttcagctct                                      >  2:1041076/1‑140 (MQ=255)                                                                                                   ttGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGagctcttcagctc                                       >  2:624915/1‑137 (MQ=255)                                                                                                   ttGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGagctcttcagctc                                       <  1:624915/137‑1 (MQ=255)                                                                                                     gTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCt                                  <  1:831477/140‑1 (MQ=255)                                                                                                     gTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCt                                  <  2:859292/140‑1 (MQ=255)                                                                                                              aTTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTcc                                   >  1:825255/1‑130 (MQ=255)                                                                                                              aTTGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTcc                                   <  2:825255/130‑1 (MQ=255)                                                                                                               ttGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGAc                        <  2:255368/140‑1 (MQ=255)                                                                                                                tGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCGTCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGAcc                       >  2:303481/1‑140 (MQ=255)                                                                                                                tGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGAcc                       <  1:994473/140‑1 (MQ=255)                                                                                                                tGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGAcc                       >  1:321766/1‑140 (MQ=255)                                                                                                                tGCCTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGAcc                       <  2:1036870/140‑1 (MQ=255)                                                                                                                  ccTAGTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCgg                     <  1:40802/140‑1 (MQ=255)                                                                                                                      gTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTa                 >  2:326535/1‑140 (MQ=255)                                                                                                                      gTACGTTCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTa                 >  2:765156/1‑140 (MQ=255)                                                                                                                      gTACGTTCATGGCGTAAAGTTCGCGAAGGTGTCCCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGATGACCGGCGTa                 >  1:9742/1‑140 (MQ=255)                                                                                                                            tCATGGCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTtct           >  2:416125/1‑140 (MQ=255)                                                                                                                                 gCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGc      >  2:1060415/1‑140 (MQ=255)                                                                                                                                 gCGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGc      >  1:1093772/1‑140 (MQ=255)                                                                                                                                  cGTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCa     <  1:416125/140‑1 (MQ=255)                                                                                                                                   gTAAAGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCaa    >  1:1093016/1‑140 (MQ=255)                                                                                                                                     aaaGTTCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAgg  >  2:103000/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       GCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGG  >  NC_000913/2339141‑2339412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑