Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,420,144	C→A	M114I (ATG→ATT)	yfcF ←	glutathione S‑transferase YfcF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,420,144	0	C	A	100.0%	209.2 / NA	59	M114I (ATG→ATT)	yfcF	glutathione S‑transferase YfcF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/34);  total (25/34)

CTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTC  >  NC_000913/2420015‑2420279                                                                                                                                  |                                                                                                                                        cTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGc                                                                                                                               >  2:292671/1‑140 (MQ=255)  tGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCt                                                                                                                              >  1:1006793/1‑140 (MQ=255)        aGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAg                                                                                                                        <  1:1081862/140‑1 (MQ=255)         gTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGc                                                                                                                       <  2:513113/140‑1 (MQ=255)         gTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGc                                                                                                                       <  1:1098664/140‑1 (MQ=255)         gTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGc                                                                                                                       <  2:152277/140‑1 (MQ=255)         gTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGc                                                                                                                       <  2:48281/140‑1 (MQ=255)          tACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGcc                                                                                                                      <  1:467447/140‑1 (MQ=255)          tACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACACTCTTCGCGGATGGGAATCAGATCGCTGCGCAGcc                                                                                                                      >  2:49760/1‑140 (MQ=255)              aaCAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGc                                                                                                                  <  2:62396/140‑1 (MQ=255)               aCAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGcc                                                                                                                 >  2:456263/1‑140 (MQ=255)                   aTGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGaa                                                                                                             >  1:183492/1‑140 (MQ=255)                    tGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAAt                                                                                                            >  2:623887/1‑140 (MQ=255)                    tGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAAt                                                                                                            >  1:1001823/1‑140 (MQ=255)                    tGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAAt                                                                                                            >  2:588105/1‑140 (MQ=255)                        cTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGt                                                                                                        >  1:736883/1‑140 (MQ=255)                         tGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTc                                                                                                       >  2:507323/1‑140 (MQ=255)                              tCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACACTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCa                                                                                                  <  1:49760/140‑1 (MQ=255)                                gcgAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCAcg                                                                                                >  1:263182/1‑140 (MQ=255)                                  gAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCAcgcg                                                                                              <  2:1012033/140‑1 (MQ=255)                                       gTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGa                                                                                         <  1:1141823/140‑1 (MQ=255)                                                cACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTaaa                                                                                >  1:59691/1‑140 (MQ=255)                                                 aCTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAAt                                                                               >  2:177514/1‑140 (MQ=255)                                                  cTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATc                                                                              >  1:945694/1‑140 (MQ=255)                                                  cTGGCTTTTCCCTCGGCCGTTAGTGGCGCTNTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATAGCTGCGAAGCCAGGCCTGAATATGTCGCGCACGCGCACGAATTTCTAAAtt                                                                              >  2:871372/1‑139 (MQ=255)                                                    ggCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCaa                                                                            >  1:763358/1‑140 (MQ=255)                                                     gCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAg                                                                           >  2:581151/1‑140 (MQ=255)                                                     gCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAg                                                                           >  2:626438/1‑140 (MQ=255)                                                     gCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAg                                                                           >  2:581168/1‑140 (MQ=255)                                                     gCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAg                                                                           >  1:771201/1‑140 (MQ=255)                                                     gCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAg                                                                           >  2:889082/1‑140 (MQ=255)                                                     gCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAg                                                                           <  1:507323/140‑1 (MQ=255)                                                     gCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAg                                                                           >  1:294766/1‑140 (MQ=255)                                                      cTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGc                                                                          <  1:1005102/140‑1 (MQ=255)                                                      cTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGc                                                                          >  2:751181/1‑140 (MQ=255)                                                      cTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGc                                                                          <  1:152884/140‑1 (MQ=255)                                                       ttttCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCg                                                                         <  2:491752/140‑1 (MQ=255)                                                          tCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGAt                                                                      >  1:1011813/1‑140 (MQ=255)                                                           cccTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATa                                                                     <  2:771201/140‑1 (MQ=255)                                                           cccTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATa                                                                     <  1:623887/140‑1 (MQ=255)                                                              tCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAAt                                                                  <  1:806380/140‑1 (MQ=255)                                                                ggCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATAc                                                                >  2:614986/1‑140 (MQ=255)                                                                 gCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACg                                                               <  1:878341/140‑1 (MQ=255)                                                                     ttAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTcc                                                           <  1:538387/140‑1 (MQ=255)                                                                               ttttttCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTg                                                 <  1:566027/140‑1 (MQ=255)                                                                                tttttCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTgg                                                <  1:405307/140‑1 (MQ=255)                                                                                       cccccGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAAt                                         <  2:1006793/140‑1 (MQ=255)                                                                                         cccGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCg                                       <  2:945694/140‑1 (MQ=255)                                                                                         cccGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCg                                       <  2:24689/140‑1 (MQ=255)                                                                                           cGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCggt                                     <  1:614986/140‑3 (MQ=255)                                                                                           cGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGAt                                     <  2:183492/140‑1 (MQ=255)                                                                                           cGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGAt                                     <  1:177514/140‑1 (MQ=255)                                                                                                aGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTcc                                <  2:763358/140‑1 (MQ=255)                                                                                                aGACAACATCCGTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTcc                                <  1:626438/140‑1 (MQ=255)                                                                                                           gTCGGACGCTCTTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGc                     <  2:1001823/140‑1 (MQ=255)                                                                                                                   ctctTCGCGGATGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCag             <  2:263182/140‑1 (MQ=255)                                                                                                                             aTGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCact   <  2:294766/140‑1 (MQ=255)                                                                                                                             aTGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCact   <  1:889082/140‑1 (MQ=255)                                                                                                                             aTGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCact   <  1:588105/140‑1 (MQ=255)                                                                                                                              tGGGAATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCactc  >  2:940045/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                        CTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTC  >  NC_000913/2420015‑2420279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑