Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,690,982	C→A	A39A (GCG→GCT)	glrK ←	sensory histidine kinase GlrK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,690,982	0	C	A	100.0%	164.4 / NA	47	A39A (GCG→GCT)	glrK	sensory histidine kinase GlrK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/19);  total (28/19)

GCCAGGGTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGT  >  NC_000913/2690848‑2691112                                                                                                                                       |                                                                                                                                   gCCAGGGTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCAtt                                                                                                                               <  2:52255/140‑1 (MQ=255) gCCAGGGTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCAtt                                                                                                                               <  2:663532/140‑1 (MQ=255) gCCAGGGTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCAtt                                                                                                                               >  1:513156/1‑140 (MQ=255)     gggTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAgg                                                                                                                           <  2:286623/140‑1 (MQ=255)      ggTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGc                                                                                                                          >  1:231066/1‑140 (MQ=255)           ggTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGc                                                                                                                     >  2:1091303/1‑140 (MQ=255)           ggTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGc                                                                                                                     >  1:533047/1‑140 (MQ=255)              cgtcCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAg                                                                                                                  <  2:567659/140‑1 (MQ=255)               gtcCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAgg                                                                                                                 <  1:894424/140‑1 (MQ=255)                tcCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGc                                                                                                                >  2:765559/1‑140 (MQ=255)                tcCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGc                                                                                                                >  2:222051/1‑140 (MQ=255)                 ccAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCt                                                                                                               >  2:1009851/1‑140 (MQ=255)                  cAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCtt                                                                                                              >  1:230545/1‑140 (MQ=255)                          aaTACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCAGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTtgccatgcc                                                                                                      <  2:478667/140‑1 (MQ=255)                                    gTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACa                                                                                            >  1:661989/1‑140 (MQ=255)                                      aaCTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAgg                                                                                          >  2:673679/1‑140 (MQ=255)                                             cTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGgcagca                                                                                   >  2:744280/1‑140 (MQ=255)                                                              cgTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGcc                                                                  >  1:875360/1‑140 (MQ=255)                                                              cgTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGcc                                                                  >  2:422191/1‑140 (MQ=255)                                                              cgTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGcc                                                                  >  2:283620/1‑140 (MQ=255)                                                                       tCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTAcc                                                         <  2:907346/140‑1 (MQ=255)                                                                        cGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATg                                                                    <  1:391536/128‑1 (MQ=255)                                                                        cGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATg                                                                    >  2:391536/1‑128 (MQ=255)                                                                         gCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAg                                                       <  2:578665/140‑1 (MQ=255)                                                                           ttCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGtt                                                     <  1:765559/140‑1 (MQ=255)                                                                                     cGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGa                                           >  1:981543/1‑140 (MQ=255)                                                                                       ggCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGc                                         >  1:859851/1‑140 (MQ=255)                                                                                                  gCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACg                              >  1:913172/1‑140 (MQ=255)                                                                                                    gTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACggg                            >  1:1114886/1‑140 (MQ=255)                                                                                                      aGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGcc                          >  2:609480/1‑140 (MQ=255)                                                                                                      aGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGcc                          >  1:1027507/1‑140 (MQ=255)                                                                                                      aGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGcc                          >  1:502981/1‑140 (MQ=255)                                                                                                      aGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGcc                          >  1:1165383/1‑140 (MQ=255)                                                                                                       gTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCa                         >  1:829494/1‑140 (MQ=255)                                                                                                       gTGCGGTTAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCa                         >  2:890138/1‑140 (MQ=255)                                                                                                             ttAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGttt                   <  2:231066/140‑1 (MQ=255)                                                                                                             ttAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGttt                   <  1:983755/140‑1 (MQ=255)                                                                                                             ttAACCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGttt                   <  2:859851/140‑1 (MQ=255)                                                                                                               aaCCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCa                 <  2:981543/140‑1 (MQ=255)                                                                                                               aaCCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCa                 <  1:1009851/140‑1 (MQ=255)                                                                                                               aaCCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCa                 <  1:222051/140‑1 (MQ=255)                                                                                                               aaCCAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCa                 <  2:68557/140‑1 (MQ=255)                                                                                                                  cAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAgg              >  1:78190/1‑140 (MQ=255)                                                                                                                  cAGCGCCGCCTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGCCCAGCGTTTCAAgg              >  2:992229/1‑140 (MQ=255)                                                                                                                      gccgccTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGtt          >  1:438678/1‑140 (MQ=255)                                                                                                                         gccTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTAct       <  2:63911/140‑1 (MQ=255)                                                                                                                         gccTGATCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTAct       <  2:269440/140‑1 (MQ=255)                                                                                                                              aTCGCTAAGAGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGt  >  2:1115125/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                   GCCAGGGTTGGGTCGTCCAGCACGCAATACTGACGGTAACTACGCTCCATCTCCAGCGCCGCGTTGGTCATCGCTTCACTGCGCCGGGCATCAATAAGCGTAGTGCGGTTAACCAGCGCCGCCTGATCGCTAAGCGCATTCAGGCTTTGCCAGGCTTGCCATGCCAGCACCAACAGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGT  >  NC_000913/2690848‑2691112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑