Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,105,439	G→T	R336L (CGC→CTC)	mltC →	membrane‑bound lytic murein transglycosylase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,105,439	0	G	T	100.0%	90.9 / NA	27	R336L (CGC→CTC)	mltC	membrane‑bound lytic murein transglycosylase C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/18);  total (9/18)

CGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAA  >  NC_000913/3105303‑3105567                                                                                                                                         |                                                                                                                                 cggcgTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCa                                                                                                                               <  2:456468/140‑1 (MQ=255) cggcgTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCa                                                                                                                               >  2:145070/1‑140 (MQ=255) cggcgTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGAAGCCGGGCGATGTTTATAAGACGCTGACGACCCTCCa                                                                                                                               >  1:548538/1‑140 (MQ=255)      ttATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCct                                                                                                                          <  2:644633/140‑1 (MQ=255)      ctATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCct                                                                                                                          >  2:818894/2‑140 (MQ=255)         tGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTg                                                                                                                       >  2:133114/1‑140 (MQ=255)              taatttccGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGaa                                                                                                                  <  2:162142/134‑1 (MQ=255)                      gCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCg                                                                                                          <  1:1039219/140‑1 (MQ=255)                       ccTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGt                                                                                                         <  1:145070/140‑1 (MQ=255)                         tataACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTa                                                                                                       <  1:188431/140‑1 (MQ=255)                         tataACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTa                                                                                                       <  1:818894/140‑1 (MQ=255)                            aaCGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTATCt                                                                                                    <  1:133114/140‑1 (MQ=255)                                gcggcgCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTATCTTtat                                                                                                <  1:1122680/140‑1 (MQ=255)                                gcggcgCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTATCTTtat                                                                                                <  2:587282/140‑1 (MQ=255)                                gcggcgCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTATCTTtat                                                                                                <  2:478385/140‑1 (MQ=255)                                gcggcgCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTATCTTtat                                                                                                <  1:983127/140‑1 (MQ=255)                                gcggcgCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTATCTTtat                                                                                                <  1:216511/140‑1 (MQ=255)                                          cagCGTGCTGCGAGTCTTTTCGAATAATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATa                                                                                      <  1:67211/140‑1 (MQ=255)                                                gctgcGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCgcgc                                                                                <  2:786953/140‑1 (MQ=255)                                                                    aTAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGa                                                            <  2:333529/140‑1 (MQ=255)                                                                    aTAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGa                                                            <  2:44842/140‑1 (MQ=255)                                                                      aaGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATa                                                          <  1:144134/140‑1 (MQ=255)                                                                                      attttataaACCCCATGCCCCGGGGCGTTTTTTATCAGCCGCTGACGCCCCCCCACCCCTCTGCGAAATCTCGCCGTTATTTTTAAAAGGTGAATCCCGCGAAAAAACCCTCCCCCCGCCGATAATTCCATTAACCGccc                                          <  2:1051824/137‑1 (MQ=255)                                                                                                 acCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCGTTAACCGCCCCTGACGATGCt                               >  2:1163046/1‑140 (MQ=255)                                                                                                  ccATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATACTc                              >  2:502571/1‑140 (MQ=255)                                                                                                   cATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCa                             >  2:889108/1‑140 (MQ=255)                                                                                                   cATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCa                             >  1:1129426/1‑140 (MQ=255)                                                                                                      gACGCCGGGCGATGTTTATCAGACGCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCTCGCAAAAATCCTACCGCCGCCGATGATTCCATTAACCGCCCCTGACGATGCCCAggg                          >  2:764811/1‑140 (MQ=255)                                                                                                       ccGCCGGGCGATTTTTATCAGACGCTGACGACCCTCCTTCCCTCGGGGAAATCTCGCCGTTATTTTTATAAAGGAAACCCCGCGAAAAAATCCTCCGGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAgggg                         <  2:138337/139‑1 (MQ=255)                                                                                                                              gCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACaa  >  2:778033/1‑140 (MQ=255)                                                                                                                              gCTGACGACCCTCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACaa  >  2:611450/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                 CGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAA  >  NC_000913/3105303‑3105567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑