Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,256,565	T→C	E29E (GAA→GAG)	cyuA ←	putative L‑cysteine desulfidase CyuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,256,565	0	T	C	100.0%	205.8 / NA	55	E29E (GAA→GAG)	cyuA	putative L‑cysteine desulfidase CyuA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/25);  total (30/25)

CCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCAAACATATTAAAAACCTTAAAATTTCAGGTAAATCAAGAAAATGCCAGGAA  >  NC_000913/3256437‑3256696                                                                                                                                 |                                                                                                                                    cccGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTGTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATcc                                                                                                                          >  2:986286/1‑140 (MQ=255) cccGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATcc                                                                                                                          >  2:1147297/1‑140 (MQ=255)     tGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAgc                                                                                                                      >  1:760838/1‑140 (MQ=255)     tGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAgc                                                                                                                      >  2:606837/1‑140 (MQ=255)     tGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAgc                                                                                                                      >  1:585251/1‑140 (MQ=255)      gCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAgcg                                                                                                                     >  1:290842/1‑140 (MQ=255)            aaCGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTCCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTGCATCCCAGCGCCGGtt                                                                                                               <  2:866432/140‑1 (MQ=255)                 tGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACt                                                                                                          <  1:1147297/140‑1 (MQ=255)                 tGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACt                                                                                                          <  2:1030814/140‑1 (MQ=255)                   aCGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTGTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTtc                                                                                                        <  1:986286/140‑1 (MQ=255)                          gACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGa                                                                                                 >  2:1139137/1‑140 (MQ=255)                            ccGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAAc                                                                                               <  2:850955/140‑1 (MQ=255)                               ttcttcATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGc                                                                                            >  2:75815/1‑140 (MQ=255)                               ttcttcATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGc                                                                                            >  1:100565/1‑140 (MQ=255)                                 cttcATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGa                                                                                          <  2:290842/140‑1 (MQ=255)                                   tcatcaGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAgg                                                                                        >  2:88551/1‑140 (MQ=255)                                      tcaGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATg                                                                                     <  2:906884/140‑1 (MQ=255)                                         gATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTaa                                                                                  >  1:826915/1‑140 (MQ=255)                                           tttGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACg                                                                                <  1:417896/140‑1 (MQ=255)                                                    aCCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATaa                                                                       >  2:687103/1‑140 (MQ=255)                                                     cccAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAAc                                                                      <  1:50493/140‑1 (MQ=255)                                                     cccAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAAc                                                                      <  1:47767/140‑1 (MQ=255)                                                            ttCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTa                                                               <  1:88551/140‑1 (MQ=255)                                                               tACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGAttt                                                                >  2:989677/1‑136 (MQ=255)                                                               tACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGAttt                                                                <  1:989677/136‑1 (MQ=255)                                                               tACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAg                                                            >  2:677885/1‑140 (MQ=255)                                                               tACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAg                                                            >  1:1042845/1‑140 (MQ=255)                                                               tACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAg                                                            >  2:960557/1‑140 (MQ=255)                                                               tACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAg                                                            >  1:274858/1‑140 (MQ=255)                                                               tACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAg                                                            <  1:761934/140‑1 (MQ=255)                                                               tACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCACCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAg                                                            >  2:400314/1‑140 (MQ=255)                                                                    gTTCCACCGCACCCTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTCCCTCCCAGCGCCGGTTTTTCTTCCTCCTGAACGGCGAGGATGTATCGCTGCCATTACGGATTTACAGTCGat                                                       <  2:1042845/140‑2 (MQ=255)                                                                      tCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATc                                                     >  2:378721/1‑140 (MQ=255)                                                                        aaCCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAg                                                            <  2:175438/131‑1 (MQ=255)                                                                        aaCCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAg                                                            >  1:175438/1‑131 (MQ=255)                                                                        aaCCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCaa                                                   <  2:585251/140‑1 (MQ=255)                                                                        aaCCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCaa                                                   >  1:582394/1‑140 (MQ=255)                                                                         aCCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCaaa                                                  >  1:437623/1‑140 (MQ=255)                                                                         aCCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCaaa                                                  >  1:647723/1‑140 (MQ=255)                                                                          ccGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCAAAc                                                 <  2:742350/140‑1 (MQ=255)                                                                             gACCTTCCAGTTCTGCCGCAGCAACCGCCTCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCa                                                    >  2:547891/1‑134 (MQ=255)                                                                             gACCTTCCAGTTCTGCCGCAGCAACCGCCTCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCa                                                    <  1:547891/134‑1 (MQ=255)                                                                             gACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCAAACata                                              >  2:729293/1‑140 (MQ=255)                                                                              aCCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCAAACatat                                             >  2:703782/1‑140 (MQ=255)                                                                                  tCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCAAACATATTaaa                                         >  1:607167/1‑140 (MQ=255)                                                                                  tCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCAAACATATTaaa                                         >  2:129320/1‑140 (MQ=255)                                                                                        tCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCAAACATATTAAAAACCtt                                   <  1:960557/140‑1 (MQ=255)                                                                                            ccGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCAAACATATTAAAAACCTTaaaa                               >  1:314353/1‑140 (MQ=255)                                                                                             cGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCAAACATATTAAAAACCTTAAAAt                              <  2:274858/140‑1 (MQ=255)                                                                                              gcagcaACCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCAAACATATTAAAAACCTTAAAAtt                             >  2:947575/1‑140 (MQ=255)                                                                                                   aaCCGCCGCCGCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCAAACATATTAAAAACCTTAAAATTTCAgg                        <  1:703782/140‑1 (MQ=255)                                                                                                   aaCCGCCGCCGCCAGCACCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCAAACATATTAAAAACCTTAAAATTTCAgg                        <  1:400314/140‑1 (MQ=255)                                                                                                     ccgccgccgCCAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCAAACATATTAAAAACCTTAAAATTTCAGGTa                      <  1:606837/140‑1 (MQ=255)                                                                                                         cgccgccAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCAAACATATTAAAAACCTTAAAATTTCAGGTAAATc                  <  2:760838/140‑1 (MQ=255)                                                                                                         cgccgccAGCGCCAGTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCAAACATATTAAAAACCTTAAAATTTCAGGTAAATc                  >  2:773117/1‑140 (MQ=255)                                                                                                                       gTGAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCAAACATATTAAAAACCTTAAAATTTCAGGTAAATCAAGAAAATGCCAgg    <  2:647723/140‑1 (MQ=255)                                                                                                                         gAAATCGGCTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCAAACATATTAAAAACCTTAAAATTTCAGGTAAATCAAGAAAATGCCAGGaa  <  2:437623/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                    CCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCTGAACGGCGAGGATGTAACGCTGCCATAACGGATTTAAAGTCGAATCAAACATATTAAAAACCTTAAAATTTCAGGTAAATCAAGAAAATGCCAGGAA  >  NC_000913/3256437‑3256696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑