Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,138,121	C→A	L305F (TTG→TTT)	gldA ←	L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,138,121	0	C	A	100.0%	230.6 / NA	64	L305F (TTG→TTT)	gldA	L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (33/31);  total (33/31)

CGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTC  >  NC_000913/4137985‑4138256                                                                                                                                         |                                                                                                                                        cggcgTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGaaaa                                                                                                                                      >  1:757866/1‑140 (MQ=255)   gcgTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAAcc                                                                                                                                    <  1:930383/140‑1 (MQ=255)   gcgTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAAcc                                                                                                                                    <  1:819017/140‑1 (MQ=255)          ccTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCa                                                                                                                             <  2:757866/140‑1 (MQ=255)          ccTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCa                                                                                                                             <  2:434892/140‑1 (MQ=255)          ccTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCa                                                                                                                             <  1:249162/140‑1 (MQ=255)          ccTGATCTGGCGTAGCGCCGCCAGGCATGTTGTGAATGGTTTCACTTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCa                                                                                                                             <  2:46125/140‑1 (MQ=255)              aTCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGc                                                                                                                         >  2:1084529/1‑140 (MQ=255)              aTCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGc                                                                                                                         >  2:223940/1‑140 (MQ=255)              aTCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCACATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCa                                                                                                                             <  1:1052569/136‑1 (MQ=255)              aTCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCACATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCa                                                                                                                             >  2:1052569/1‑136 (MQ=255)                      tCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCa                                                                                                                 <  2:41550/140‑1 (MQ=255)                        gcgcCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGc                                                                                                               >  2:366756/1‑140 (MQ=255)                         cgccgcCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCt                                                                                                              >  2:400067/1‑140 (MQ=255)                         cgccgcCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCt                                                                                                              >  2:597065/1‑140 (MQ=255)                              ccAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGt                                                                                                         >  1:1107579/1‑140 (MQ=255)                                            aaTGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCa                                                                                           >  2:815612/1‑140 (MQ=255)                                               ggTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCg                                                                                        <  2:429225/140‑1 (MQ=255)                                                    cACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCa                                                                                   <  1:815612/140‑1 (MQ=255)                                                    cACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCa                                                                                   >  1:957042/1‑140 (MQ=255)                                                     cccTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCAt                                                                                  <  2:957042/139‑1 (MQ=255)                                                           tGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCa                                                                            >  2:434416/1‑140 (MQ=255)                                                           tGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCa                                                                            >  2:217372/1‑140 (MQ=255)                                                              acaTGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGaa                                                                         <  1:400067/140‑1 (MQ=255)                                                                 tGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCa                                                                      >  1:237440/1‑140 (MQ=255)                                                                  gCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTc                                                                              <  2:1074191/131‑1 (MQ=255)                                                                  gCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTc                                                                              >  1:1074191/1‑131 (MQ=255)                                                                  gCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAg                                                                     >  1:202236/1‑140 (MQ=255)                                                                   ccGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGc                                                                    >  2:533726/1‑140 (MQ=255)                                                                    cGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCt                                                                   >  2:1117373/1‑140 (MQ=255)                                                                       ttCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGCGCAGCTACGGTTTCGATTTCCTCCACCGGCACATTTTCCAGAACCAGCTGCg                                                                <  1:279449/140‑1 (MQ=255)                                                                        tCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGt                                                               <  2:722431/140‑1 (MQ=255)                                                                          tGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCa                                                             <  2:237440/140‑1 (MQ=255)                                                                            ccACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGc                                                           <  1:565609/140‑1 (MQ=255)                                                                                      cATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATg                                                 >  1:278287/1‑140 (MQ=255)                                                                                       aTTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGc                                                <  1:1117373/140‑1 (MQ=255)                                                                                       aTTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGc                                                <  1:597065/140‑1 (MQ=255)                                                                                         tttCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCa                                              <  1:223940/140‑1 (MQ=255)                                                                                            cGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACtt                                           >  2:993547/1‑140 (MQ=255)                                                                                                gggACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTc                                       <  1:217372/140‑1 (MQ=255)                                                                                                  gACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCAc                                     <  2:202236/140‑1 (MQ=255)                                                                                                  gACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCAc                                     <  2:688223/140‑1 (MQ=255)                                                                                                  gACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCAc                                     <  1:212606/140‑1 (MQ=255)                                                                                                     aTCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGt                                  <  2:256358/140‑1 (MQ=255)                                                                                                       cttcttTAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGa                                <  2:278287/140‑1 (MQ=255)                                                                                                             tAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCAc                                     >  1:263831/1‑129 (MQ=255)                                                                                                             tAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCAc                                     <  2:263831/129‑1 (MQ=255)                                                                                                             tAATATCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAg                          >  2:575529/1‑140 (MQ=255)                                                                                                                  tCCAGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATg                     >  2:35657/1‑140 (MQ=255)                                                                                                                     aGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATgcgc                  <  1:1084529/140‑1 (MQ=255)                                                                                                                     aGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATgcgc                  >  2:486305/1‑140 (MQ=255)                                                                                                                     aGTTGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATgcgc                  >  1:369207/1‑140 (MQ=255)                                                                                                                       ttGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGt                >  1:909220/1‑140 (MQ=255)                                                                                                                       ttGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGt                >  2:520170/1‑140 (MQ=255)                                                                                                                        tGAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTc               >  2:788767/1‑140 (MQ=255)                                                                                                                         gAGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTcc              >  2:180728/1‑140 (MQ=255)                                                                                                                          aGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCg             >  1:945371/1‑140 (MQ=255)                                                                                                                          aGCGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCg             >  1:1148757/1‑140 (MQ=255)                                                                                                                            cGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTc               >  2:575624/1‑136 (MQ=255)                                                                                                                            cGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTc               <  1:575624/136‑1 (MQ=255)                                                                                                                            cGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCggg           >  2:685057/1‑140 (MQ=255)                                                                                                                            cGAGAGTTATTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCggg           >  2:745290/1‑140 (MQ=255)                                                                                                                                    aTTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGt   <  1:486305/140‑1 (MQ=255)                                                                                                                                    aTTGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGt   <  1:366756/140‑1 (MQ=255)                                                                                                                                     ttGGAAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTc  >  2:1057256/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTC  >  NC_000913/4137985‑4138256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑