Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	774,979	C→A	L76L (CTC→CTA)	ybgC →	esterase/thioesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	774,979	0	C	A	100.0%	264.4 / NA	75	L76L (CTC→CTA)	ybgC	esterase/thioesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (40/35);  total (40/35)

ATGAAAGAGCACGCACAGAGATGCTGCGTCATCATCACTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTCGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAA  >  NC_000913/774846‑775106                                                                                                                                      |                                                                                                                                aTGAAAGAGCACGCACAGAGATGCTGCGTCATCATCACTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATa                                                                                                                           >  1:979334/1‑140 (MQ=255)   gAAAGAGCACGCACAGAGATGCTGCGTCATCATCACTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACa                                                                                                                         <  1:1153964/140‑1 (MQ=255)        agCACGCACAGAGATGCTGCGTCATCATCACTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACt                                                                                                                     >  1:117130/1‑139 (MQ=255)        agCACGCACAGAGATGCTGCGTCATCATCACTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACt                                                                                                                     <  2:117130/139‑1 (MQ=255)                    gaTGCTGCGTCATCATCACTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCaa                                                                                                        >  2:814767/1‑140 (MQ=255)                     aTGCTGCGTCATCATCACTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAAt                                                                                                       >  1:939075/1‑140 (MQ=255)                       gctgcGTCATCATCACTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGc                                                                                                     >  2:124377/1‑140 (MQ=255)                        ctgcGTCATCATCACTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCg                                                                                                    >  1:366716/1‑140 (MQ=255)                         tgcGTCATCATCACTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGt                                                                                                   >  2:9091/1‑140 (MQ=255)                         tgcGTCATCATCACTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGt                                                                                                   >  2:429396/1‑140 (MQ=255)                           cGTCATCATCACTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTgg                                                                                                 <  1:435589/140‑1 (MQ=255)                             tcatcatcaCTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCa                                                                                               >  2:309096/1‑140 (MQ=255)                             tcatcatcaCTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCa                                                                                               >  2:1093190/1‑140 (MQ=255)                                 catcaCTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACctc                                                                                           >  2:548708/1‑140 (MQ=255)                                  atcaCTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACctct                                                                                          >  1:667003/1‑140 (MQ=255)                                     aCTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTg                                                                                       >  2:470235/1‑140 (MQ=255)                                          agtcagCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCAcc                                                                                             <  2:129987/129‑1 (MQ=255)                                          agtcagCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCAcc                                                                                             >  1:129987/1‑129 (MQ=255)                                           gtcagCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTc                                                                                 >  1:330389/1‑140 (MQ=255)                                            tcagCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCa                                                                                >  1:834277/1‑140 (MQ=255)                                            tcagCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCa                                                                                >  1:1088346/1‑140 (MQ=255)                                             cagcagGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCAc                                                                               <  2:979334/140‑1 (MQ=255)                                             cagcagGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCAc                                                                               <  2:939075/140‑1 (MQ=255)                                              agcagGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACg                                                                              <  1:1150199/140‑1 (MQ=255)                                              agcagGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACA‑CAATGCGTGGCACCTCTTTGGTTTTCACGc                                                                             <  1:371221/140‑1 (MQ=255)                                                cagGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGcg                                                                            <  2:239159/140‑2 (MQ=255)                                                cagGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCa                                                                            <  1:548708/140‑1 (MQ=255)                                                cagGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCa                                                                            <  1:1093190/140‑1 (MQ=255)                                                cagGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCa                                                                            <  2:354170/140‑1 (MQ=255)                                                cagGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCa                                                                            <  1:309096/140‑1 (MQ=255)                                                cagGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCa                                                                            <  2:269127/140‑1 (MQ=255)                                                cagGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCa                                                                            >  1:159533/1‑140 (MQ=255)                                                   gcgcTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACg                                                                         >  2:909714/1‑140 (MQ=255)                                                     gcTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTa                                                                       <  1:964680/140‑1 (MQ=255)                                                             cTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAAc                                                               <  1:1124362/140‑1 (MQ=255)                                                              tGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACg                                                              >  1:171058/1‑140 (MQ=255)                                                               gAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGc                                                             <  2:800907/140‑1 (MQ=255)                                                                  cgcgTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCga                                                          >  2:808175/1‑140 (MQ=255)                                                                  cgcgTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCga                                                          <  2:387744/140‑1 (MQ=255)                                                                      ttGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAAc                                                               >  2:288868/1‑131 (MQ=255)                                                                      ttGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAAc                                                               <  1:288868/131‑1 (MQ=255)                                                                       tGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATa                                                     <  2:296172/140‑1 (MQ=255)                                                                       tGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATa                                                     <  2:929018/140‑1 (MQ=255)                                                                         ccTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACt                                                   <  2:159533/140‑1 (MQ=255)                                                                          cTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACtt                                                  >  1:473059/1‑140 (MQ=255)                                                                           tttGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACttt                                                 >  1:390092/1‑140 (MQ=255)                                                                            ttGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTg                                                <  1:5955/140‑1 (MQ=255)                                                                                 gTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCtgaa                                           <  1:808175/140‑1 (MQ=255)                                                                                    cGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCtgaatga                                        >  2:1102028/1‑140 (MQ=255)                                                                                      tAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAg                                      <  2:390092/140‑1 (MQ=255)                                                                                        aaaTGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCa                                    <  2:552448/140‑1 (MQ=255)                                                                                         aaTGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCag                                   <  2:171058/140‑1 (MQ=255)                                                                                         aaTGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCag                                   <  1:124377/140‑1 (MQ=255)                                                                                          aTGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCaga                                  <  1:1070664/140‑1 (MQ=255)                                                                                            gACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAgg                                <  1:909714/140‑1 (MQ=255)                                                                                            gACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAgg                                <  2:744238/140‑1 (MQ=255)                                                                                              cGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGtt                              >  1:440346/1‑140 (MQ=255)                                                                                                gTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCt                            >  2:360532/1‑140 (MQ=255)                                                                                                       atTACGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGgttgtt                     >  1:272537/1‑140 (MQ=255)                                                                                                           cGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGCATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCg                 >  2:5589/1‑140 (MQ=255)                                                                                                           cGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGgttgt                      >  2:650164/1‑135 (MQ=255)                                                                                                           cGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGgttgt                      <  1:650164/135‑1 (MQ=255)                                                                                                           cGCACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCg                 >  2:729444/1‑140 (MQ=255)                                                                                                             cACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGtt               >  2:101079/1‑140 (MQ=255)                                                                                                             cACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGtt               >  2:112112/1‑140 (MQ=255)                                                                                                             cACCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGtt               >  1:560349/1‑140 (MQ=255)                                                                                                              aCCTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTg              >  2:40210/1‑140 (MQ=255)                                                                                                               ccTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGa             >  2:1130239/1‑140 (MQ=255)                                                                                                                cTGCGCGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGAc            <  1:821641/140‑1 (MQ=255)                                                                                                                  gcgcGGCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGAccc          >  1:1130825/1‑140 (MQ=255)                                                                                                                       gCTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCa     <  2:641350/140‑1 (MQ=255)                                                                                                                        cTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGAcc           <  2:603788/133‑1 (MQ=255)                                                                                                                        cTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGAcc           >  1:603788/1‑133 (MQ=255)                                                                                                                        cTCGACGATATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCaa    >  2:706541/1‑140 (MQ=255)                                                                                                                          cgacgaTATGCTAGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCaaaa  >  1:470125/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                ATGAAAGAGCACGCACAGAGATGCTGCGTCATCATCACTTCAGTCAGCAGGCGCTGATGGCTGAACGCGTTGCCTTTGTGGTACGTAAAATGACGGTGGAATATTACGCACCTGCGCGGCTCGACGATATGCTCGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAACGCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAA  >  NC_000913/774846‑775106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑