Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	796,327	G→T	A156S (GCT→TCT)	modB →	molybdate ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	796,327	0	G	T	100.0%	214.0 / NA	61	A156S (GCT→TCT)	modB	molybdate ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/33);  total (28/33)

CAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGC  >  NC_000913/796190‑796457                                                                                                                                          |                                                                                                                                   cAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCt                                                                                                                                  >  2:940440/1‑140 (MQ=255)    ttCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCtttt                                                                                                                               >  2:702486/1‑140 (MQ=255)       gTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCt                                                                                                                            <  2:674805/140‑1 (MQ=255)            gcgcTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTtc                                                                                                                       >  1:185618/1‑140 (MQ=255)             cgcTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTtct                                                                                                                      <  1:636422/140‑1 (MQ=255)              gcTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTtctc                                                                                                                     <  2:448385/140‑1 (MQ=255)                 ggAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCg                                                                                                                  <  1:940440/140‑1 (MQ=255)                   aaGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGt                                                                                                                <  2:77862/140‑1 (MQ=255)                            aTGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGt                                                                                                       >  1:530677/1‑140 (MQ=255)                              gTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGc                                                                                                     <  1:148199/140‑1 (MQ=255)                                    cTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCAt                                                                                               <  2:1081327/140‑1 (MQ=255)                                       gAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCAc                                                                                            >  2:311526/1‑140 (MQ=255)                                             gCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAAc                                                                                                  >  2:410050/1‑128 (MQ=255)                                             gCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAAc                                                                                                  <  1:410050/128‑1 (MQ=255)                                             gCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTtgt                                                                                      >  1:686480/1‑140 (MQ=255)                                              ccGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTtgtg                                                                                     >  2:1114927/1‑140 (MQ=255)                                               cGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTgtgt                                                                                    <  1:702486/140‑1 (MQ=255)                                                       cacTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACAtt                                                                            <  2:176027/140‑1 (MQ=255)                                                       cacTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACAtt                                                                            >  2:109142/1‑140 (MQ=255)                                                           gggggCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTg                                                                        >  2:114499/1‑140 (MQ=255)                                                                   ggCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAAcg                                                                >  2:433863/1‑140 (MQ=255)                                                                    gcgcTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAAcgc                                                               >  1:390762/1‑140 (MQ=255)                                                                        tGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAAc                                                           >  2:152106/1‑140 (MQ=255)                                                                           cgcgTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCAt                                                        <  2:919450/140‑1 (MQ=255)                                                                              gTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTcc                                                     <  2:185618/140‑1 (MQ=255)                                                                                tttCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCtt                                                   <  1:1133753/140‑1 (MQ=255)                                                                                tttCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCtt                                                   <  1:389236/140‑1 (MQ=255)                                                                                   cTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTg                                                <  2:572762/140‑1 (MQ=255)                                                                                    tttACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGc                                               <  2:95146/140‑1 (MQ=255)                                                                                    tttACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGc                                               <  2:70389/140‑1 (MQ=255)                                                                                    tttACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGc                                               <  1:294268/140‑1 (MQ=255)                                                                                    tttACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGc                                               <  2:530677/140‑1 (MQ=255)                                                                                    tttACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGc                                               <  1:109142/140‑1 (MQ=255)                                                                                     ttACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGcc                                              <  2:390762/140‑1 (MQ=255)                                                                                          aTCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTccgc                                               <  1:493791/134‑4 (MQ=255)                                                                                          aTCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGta                                         <  1:826151/140‑1 (MQ=255)                                                                                          aTCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCCGc                                               >  2:493791/1‑134 (MQ=255)                                                                                           tCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGTTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGtat                                        >  1:34796/1‑140 (MQ=255)                                                                                             aCGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGc                                               >  2:701182/1‑131 (MQ=255)                                                                                             aCGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGc                                               <  1:691854/131‑1 (MQ=255)                                                                                             aCGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGc                                               >  2:691854/1‑131 (MQ=255)                                                                                             aCGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGc                                               <  1:701182/131‑1 (MQ=255)                                                                                              cGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGtata                                       >  2:174999/1‑138 (MQ=255)                                                                                              cGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGtata                                       <  1:174999/138‑1 (MQ=255)                                                                                               gTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAccc                                    <  1:85592/140‑1 (MQ=255)                                                                                               gTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAccc                                    >  2:85592/1‑140 (MQ=255)                                                                                                  aCCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAccc                                    <  1:774133/137‑1 (MQ=255)                                                                                                  aCCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATAccc                                    >  2:774133/1‑137 (MQ=255)                                                                                                  aCCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGa                                 >  1:457546/1‑140 (MQ=255)                                                                                                   ccGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGAt                                >  1:297180/1‑140 (MQ=255)                                                                                                     gCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGATcc                              >  2:39964/1‑140 (MQ=255)                                                                                                         aCCTTACCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGATCCAGAc                          >  2:1070202/1‑140 (MQ=255)                                                                                                              aCCGGGAATTATTGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGATCCAGACCCCcg                     <  1:311526/140‑1 (MQ=255)                                                                                                                     attattGTTGGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGATCCAGACCCCCGGCGGGGa              >  2:329934/1‑140 (MQ=255)                                                                                                                           gttgGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGATCCAGACCCCCGGCGGGGAAAGTgg        <  2:686480/140‑1 (MQ=255)                                                                                                                             tgGTACGGTACTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAg      <  1:114499/140‑1 (MQ=255)                                                                                                                               gtacggtacTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGATCCAGACCCCcggc                   >  1:182522/1‑125 (MQ=255)                                                                                                                               gtacggtacTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGATCCAGACCCCcggc                   <  2:182522/125‑1 (MQ=255)                                                                                                                                 acggtacTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAgcggc  >  2:672673/1‑140 (MQ=255)                                                                                                                                   ggtacTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGATCCAGACCCCCGGCGGGGAAAGTgg        >  1:965720/1‑132 (MQ=255)                                                                                                                                   ggtacTGTCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGATCCAGACCCCCGGCGGGGAAAGTgg        <  2:965720/132‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   CAATTCGTCTGGCGCTGGAAGGGGTTGATGTCAAACTGGAACAGGCCGCAAGAACACTGGGGGCCGGGCGCTGGCGCGTTTTCTTTACTATCACGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGC  >  NC_000913/796190‑796457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑