Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	170,124	C→A	A116E (GCG→GAG)	fhuC →	iron(III) hydroxamate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	170,124	0	C	A	100.0%	87.4 / NA	26	A116E (GCG→GAG)	fhuC	iron(III) hydroxamate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/10);  total (16/10)

GCCCAACCGCTGGAAAGCTGGAGCAGCAAAGCGTTTGCCCGCAAAGTGGCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCT  >  NC_000913/169991‑170259                                                                                                                                      |                                                                                                                                        gCCCAACCGCTGGAAAGCTGGAGCAGCAAAGCGTTTGCCCGCAAAGTGGCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGAGCTggg                                                                                                                                   <  2:620784/140‑1 (MQ=255)       ccGCTGGAAAGCTGGAGCAGCAAAGCGTTTGCCCGCAAAGTGGCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGAGCTGGGGCGCtt                                                                                                                             <  2:103919/140‑1 (MQ=255)                    ggAGCAGCAAAGCGTTTGCCCGCAAAGTGGCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGAt                                                                                                                <  1:223723/140‑1 (MQ=255)                                cGTTTGCCCGCAAAGTGGCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGaa                                                                                                          >  1:504602/1‑134 (MQ=255)                                cGTTTGCCCGCAAAGTGGCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGaa                                                                                                          <  2:504602/134‑1 (MQ=255)                                 gTTTGCCCGCAAAGTGGCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCg                                                                                                   <  2:296770/140‑1 (MQ=255)                                     gCCCGCAAAGTGGCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGa                                                                                               >  2:65519/1‑140 (MQ=255)                                       ccGCAAAGTGGCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAg                                                                                             >  1:594418/1‑140 (MQ=255)                                                    tATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGt                                                                                <  1:158178/140‑1 (MQ=255)                                                           cGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTa                                                                         >  1:907853/1‑140 (MQ=255)                                                                                 aGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTgg                                                   >  1:378186/1‑140 (MQ=255)                                                                                  gggATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGt                                                  <  2:907853/140‑1 (MQ=255)                                                                                  gggATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGt                                                  <  2:391402/140‑1 (MQ=255)                                                                                            tgcgtgAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGtctc                                        >  1:25857/1‑140 (MQ=255)                                                                                              cgtgAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGtctctc                                      >  1:524411/1‑140 (MQ=255)                                                                                                     tggtggCGATTGGTCGTTACCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTggcggc                               <  2:594418/140‑1 (MQ=255)                                                                                                       gtggCGATTGGTCGTTACCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGa                             >  2:503367/1‑140 (MQ=255)                                                                                                              ttGGTCGTTACCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAg                      <  2:25857/140‑1 (MQ=255)                                                                                                                 gTCGTTACCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCgg                   >  2:413972/1‑140 (MQ=255)                                                                                                                 gTCGTTACCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCgg                   >  2:822703/1‑140 (MQ=255)                                                                                                                       aCCCGTGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTgg             >  2:734199/1‑140 (MQ=255)                                                                                                                            tGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGc        >  2:234563/1‑140 (MQ=255)                                                                                                                            tGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGc        >  2:297253/1‑140 (MQ=255)                                                                                                                            tGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGc        >  2:170077/1‑140 (MQ=255)                                                                                                                            tGGCATGGCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGc        >  1:260272/1‑140 (MQ=255)                                                                                                                                  ggCGAGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCt  >  2:217167/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        GCCCAACCGCTGGAAAGCTGGAGCAGCAAAGCGTTTGCCCGCAAAGTGGCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCT  >  NC_000913/169991‑170259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑