Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	766,659	G→T	G226C (GGC→TGC)	mngA →	2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	766,659	0	G	T	100.0%	71.4 / NA	21	G226C (GGC→TGC)	mngA	2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/7);  total (14/7)

ACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATT  >  NC_000913/766528‑766793                                                                                                                                    |                                                                                                                                       aCGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACTGCATTGaa                                                                                                                                >  2:573466/1‑140 (MQ=255)  cgcgCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACTGCATTGAAg                                                                                                                               >  1:545879/1‑140 (MQ=255)   gcgcCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACTGCATTGAAgg                                                                                                                              >  2:266955/1‑140 (MQ=255)         aCTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACTGCATTGAAGGGCGttt                                                                                                                        <  1:92389/140‑1 (MQ=255)             tCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACTGCATTGAAggg                                                                                                                             >  1:119803/1‑131 (MQ=255)             tCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACTGCATTGAAggg                                                                                                                             <  2:119803/131‑1 (MQ=255)              cGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACTGCATTGAAGGGCGTTTAAcgg                                                                                                                   <  2:667937/140‑1 (MQ=255)              cGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACTGCATTGAAGGGCGTTTAAcgg                                                                                                                   <  1:884732/140‑1 (MQ=255)                      tAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACTGCATTGAAGGGCGTTTAACGGCGGATCaa                                                                                                           <  1:559683/140‑1 (MQ=255)                      tAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACTGCATTGAAGGGCGTTTAACGGCGGATCaa                                                                                                           <  1:866579/140‑1 (MQ=255)                           gCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACTGCATTGAAGGGCGTTTAACGGCGGATCAACTCaa                                                                                                      <  1:266955/140‑1 (MQ=255)                                                   acCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACTGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGc                                                                              >  2:82095/1‑140 (MQ=255)                                                   acCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACTGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGAGCAACCGCCTGTATTTTTGc                                                                              >  1:762048/1‑140 (MQ=255)                                                             cTGCGGAATTTCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACTGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTc                                                                    >  2:19884/1‑140 (MQ=255)                                                             cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACTGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTc                                                                    >  2:339115/1‑140 (MQ=255)                                                                 ggAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACTGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGGCGccc                                                                >  2:87196/1‑139 (MQ=255)                                                                  gAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACTGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCAt                                                               >  1:387645/1‑140 (MQ=255)                                                                       tCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACTGCATTGAAGGGCGTTTAACGGCGGTTTAAATCCATAGTGCAAACCCCTGTATTTTTTCGGGTTAAGTCGcccttcacg                                                          >  2:734972/1‑133 (MQ=255)                                                                                       cacAACCTCGGCATAATTTTTTAGGTAAAAAAACAGGGCGCTACCTGCATGGAGGGGTTTTTAGCGGGGGATAAACTAAATAGTGAAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTaa                                          <  2:208728/138‑1 (MQ=255)                                                                                                ggCGTAATTGTTTACGTAAAAAAACAAGGCGCTAACTGCATTGAAGGGCTTTAAACGGGGAACCAACTAAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTcc                                 <  2:491736/140‑1 (MQ=255)                                                                                                            tACGTAAAAAAACAGGGCGCTAACTGCTTGGAGGGGCTTTAAGGGGGGGATCACCTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGt                     <  2:545879/140‑1 (MQ=255)                                                                                                               ttaaaaaaaCAAGGCGCTACCTGATTTGAGGGGCTTTAAGCGGCGGATCAACTAAAGGTTCCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGcc                  <  2:659225/136‑1 (MQ=255)                                                                                                               gTTGAAAAACAAGGCGCTAACTGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGcc                  >  1:659225/1‑140 (MQ=255)                                                                                                                ttGAAAAACAAGGCGCTAACTGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGGGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCt                 >  1:112037/1‑140 (MQ=255)                                                                                                                  gAAAAACAAGGCGCTAACTGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGGGAGCGGTTTAATGGCATTCCCGCGCTTTCAGTGCCTGt               >  2:80466/1‑140 (MQ=255)                                                                                                                               cTAACTGCATGGAGGGGTGTTAAACGGGGAATAACCTAAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGAtt  <  1:19884/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       ACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATT  >  NC_000913/766528‑766793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑