Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,339,065	G→C	A119G (GCA→GGA)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,339,065	0	G	C	100.0%	71.8 / NA	20	A119G (GCA→GGA)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (15/5);  total (15/5)

CGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACC  >  NC_000913/2338953‑2339198                                                                                                                 |                                                                                                                                      cgtattttttcCCGCCCCGTCATAGTTATCAACAAAATCGCCCGTCTTTTTTTCGAGAGCGCCCATCAGTTCGGGGGCATTTTCCCCCAGACGGTTTTCCGTATACCGCTTTCCCGCCGCAGAGTCGCCGTCGATAGAAc                                                                                                            <  2:793324/135‑1 (MQ=255) cGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAAc                                                                                                            >  2:131256/1‑140 (MQ=255) cGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAAc                                                                                                            >  1:901063/1‑140 (MQ=255)                    tCATATTTATCAACAAAATCGCCCGTCTTTTTTTCGAGATCGGCCATCATTTCATGGGCATTTTCCGCCAGACGGTTTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTc                                                                                         <  2:254430/140‑1 (MQ=255)                            aTCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCa                                                                                 >  1:254430/1‑140 (MQ=255)                               aaCGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCatat                                                                              >  1:225036/1‑140 (MQ=255)                                aCGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCAtata                                                                             >  1:28049/1‑140 (MQ=255)                                 cGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATaa                                                                            >  2:206316/1‑140 (MQ=255)                                 cGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATaa                                                                            >  1:492252/1‑140 (MQ=255)                                     aTCGCCCGTCTTTTTTTCGAGACCGCCCATCATTTCAGGGGCATTTTCCGCCAGACGGATTCCCGTATAACCCTTCCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCa                                                                        <  2:237626/140‑1 (MQ=255)                                          ccGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCgag                                                                   >  1:237626/1‑140 (MQ=255)                                                    ttCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTgcg                                                         >  1:389934/1‑140 (MQ=255)                                                      cGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGcc                                                       <  1:131256/140‑1 (MQ=255)                                                        agaTCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCAt                                                     >  2:150778/1‑140 (MQ=255)                                                                  tCATTTCATGGGCATTTTCCGCCAGACGGATTTCCGTAAAACTCATCCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATc                                           <  1:745057/140‑1 (MQ=255)                                                                                aTTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGAccgc                             >  1:909100/1‑140 (MQ=255)                                                                                     cGCCAGAGGGTTTTCGTTATACCGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGt                        <  1:586666/140‑1 (MQ=255)                                                                                     cGCCAGACGGTTTTCCGTATAACGCTTTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGt                        <  1:849245/140‑1 (MQ=255)                                                                                     cGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGt                        <  1:150778/140‑1 (MQ=255)                                                                                         aGACGTTTTCCCGTAAACCGCTTCCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCAcc                    <  2:28049/140‑1 (MQ=255)                                                                                               ttttCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATgggg              <  1:381718/139‑1 (MQ=255)                                                                                               aTTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATgggg              <  1:206316/140‑1 (MQ=255)                                                                                                tttCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGa             <  2:492252/140‑1 (MQ=255)                                                                                                        taACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTAttt     >  2:7926/1‑140 (MQ=255)                                                                                                           cGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTAcc  >  1:729590/1‑140 (MQ=255)                                                                                                           cGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTAcc  >  1:155387/1‑140 (MQ=255)                                                                                                           cGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTAcc  >  2:488064/1‑140 (MQ=255)                                                                                                                 |                                                                                                                                      CGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACC  >  NC_000913/2338953‑2339198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑