Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,512,652	G→T	L19I (CTC→ATC)	mntH ←	Mn(2(+))/Fe(2(+)): H(+) symporter MntH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,512,652	0	G	T	100.0%	242.2 / NA	67	L19I (CTC→ATC)	mntH	Mn(2(+))/Fe(2(+)): H(+) symporter MntH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (39/28);  total (39/28)

GCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGAT  >  NC_000913/2512519‑2512786                                                                                                                                      |                                                                                                                                       gCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCAt                                                                                                                                  >  2:828877/1‑140 (MQ=255) gCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCAt                                                                                                                                  >  2:140823/1‑140 (MQ=255)      cAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTgc                                                                                                                             >  2:725711/1‑140 (MQ=255)            ggCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGcgccg                                                                                                                       >  1:559498/1‑140 (MQ=255)             gCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGcgccgc                                                                                                                      >  1:208878/1‑140 (MQ=255)             gCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGcgccgc                                                                                                                      >  2:767127/1‑140 (MQ=255)                cAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCg                                                                                                                   <  1:457543/140‑1 (MQ=255)                  aaCGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTc                                                                                                                 <  2:908989/140‑1 (MQ=255)                   aCGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTcc                                                                                                                >  2:784295/1‑140 (MQ=255)                       cAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCgctg                                                                                                            >  2:610603/1‑140 (MQ=255)                          cccACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTa                                                                                                         <  1:778741/140‑1 (MQ=255)                             acaGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTActc                                                                                                      >  1:531694/1‑140 (MQ=255)                              caGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTActct                                                                                                     >  1:53667/1‑140 (MQ=255)                                 tAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCaa                                                                                                  >  2:3797/1‑140 (MQ=255)                                    cTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAAcgc                                                                                               >  1:557550/1‑140 (MQ=255)                                    cTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAAcgc                                                                                               >  2:72876/1‑140 (MQ=255)                                      gATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGa                                                                                             <  1:773196/140‑1 (MQ=255)                                          gCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGt                                                                                         <  1:612234/140‑1 (MQ=255)                                           ccGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGtt                                                                                        >  1:536405/1‑140 (MQ=255)                                           ccGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGtt                                                                                        >  1:67610/1‑140 (MQ=255)                                           ccGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGtt                                                                                        >  2:87649/1‑140 (MQ=255)                                              aaGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGt                                                                                     <  1:40000/140‑1 (MQ=255)                                                gCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCa                                                                                   <  2:536405/140‑1 (MQ=255)                                                      aCCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTtgt                                                                             <  2:67610/140‑1 (MQ=255)                                                      aCCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTtgt                                                                             <  2:531694/140‑1 (MQ=255)                                                      aCCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTtgt                                                                             <  2:269773/140‑1 (MQ=255)                                                      aCCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTtgt                                                                             <  2:208878/140‑1 (MQ=255)                                                        ccGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGc                                                                           <  1:799332/140‑1 (MQ=255)                                                        ccGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGc                                                                           <  2:559498/140‑1 (MQ=255)                                                        ccGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGc                                                                           >  2:4978/1‑140 (MQ=255)                                                          gCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCct                                                                         >  2:191513/1‑140 (MQ=255)                                                           ccTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCctc                                                                        >  1:191889/1‑140 (MQ=255)                                                           ccTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCctc                                                                        >  1:84380/1‑140 (MQ=255)                                                            cTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCctct                                                                       <  1:767127/140‑1 (MQ=255)                                                                 tatTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAAc                                                                  <  1:736330/140‑1 (MQ=255)                                                                 tatTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAAc                                                                  <  1:784295/140‑1 (MQ=255)                                                                      gTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGc                                                             >  1:65541/1‑140 (MQ=255)                                                                      gTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGc                                                             >  2:44981/1‑140 (MQ=255)                                                                      gTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGc                                                             >  1:662613/1‑140 (MQ=255)                                                                        cGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCt                                                           <  1:855018/140‑1 (MQ=255)                                                                         gCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCtt                                                          >  1:291264/1‑140 (MQ=255)                                                                         gCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCtt                                                          >  2:609458/1‑140 (MQ=255)                                                                          cAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCttt                                                         >  1:82539/1‑140 (MQ=255)                                                                          cAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCttt                                                         <  2:291264/140‑1 (MQ=255)                                                                             aGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCt                                                      <  1:4978/140‑1 (MQ=255)                                                                             aGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCt                                                      <  1:610603/140‑1 (MQ=255)                                                                              gTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTa                                                     <  1:309431/140‑1 (MQ=255)                                                                                   cGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGttt                                                <  2:82539/140‑1 (MQ=255)                                                                                       aTCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATg                                            >  1:658583/1‑140 (MQ=255)                                                                                        tCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGc                                           <  2:502297/140‑1 (MQ=255)                                                                                         cGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCt                                          >  2:234730/1‑140 (MQ=255)                                                                                         cGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCt                                          >  2:132010/1‑140 (MQ=255)                                                                                             ataACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGc                                      <  1:880552/140‑1 (MQ=255)                                                                                               aaCCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCa                                    <  1:598323/140‑1 (MQ=255)                                                                                               aaCCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCa                                    >  1:577139/1‑140 (MQ=255)                                                                                                aCCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCaa                                   >  1:518330/1‑140 (MQ=255)                                                                                                     tCGCCGCAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACgag                              >  2:79682/1‑140 (MQ=255)                                                                                                       gccgcAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGaa                            <  1:132010/140‑1 (MQ=255)                                                                                                         cgcAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATg                          >  1:626804/1‑140 (MQ=255)                                                                                                         cgcAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATg                          >  1:673610/1‑140 (MQ=255)                                                                                                           cAATGAACGCAGGTCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGAt                        >  1:299233/1‑140 (MQ=255)                                                                                                                        tCCCATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCAtt           >  1:447568/1‑140 (MQ=255)                                                                                                                           cATTAATGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTaa        <  2:299233/140‑1 (MQ=255)                                                                                                                                aTGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGa   <  1:3797/140‑1 (MQ=255)                                                                                                                                 tGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGAt  >  2:576021/1‑140 (MQ=255)                                                                                                                                 tGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGAt  >  1:709545/1‑140 (MQ=255)                                                                                                                                 tGCGATCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGAt  >  1:857866/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       GCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGAT  >  NC_000913/2512519‑2512786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑