Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,758,577	G→T	N8K (AAC→AAA)	yfjH ←	CP4‑57 prophage; protein YfjH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,758,577	0	G	T	100.0%	74.0 / NA	22	N8K (AAC→AAA)	yfjH	CP4‑57 prophage; protein YfjH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/13);  total (9/13)

CGGACATGTGATAGGCGAGATCACGTCGTTTTCTACAAATTTGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGGTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCAATGAGAGGCAACAAGAATGAACAATCGATCGGCCGTTAGAATACTACGGTTACCAGCGGTTATCCAAAAAACAGGTATG  >  NC_000913/2758450‑2758706                                                                                                                                |                                                                                                                                  cgGACATGTGATAGGCGAGATCACGTCGTTTTCTACAAATTTGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGTTTTTTTGCCTTa                                                                                                                       <  1:691768/140‑1 (MQ=255)     cATGTGATAGGCGAGATCACGTCGTTTTCTACAAATTTGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGa                                                                                                                   <  2:93594/140‑1 (MQ=255)        gtgATAGGCGAGATCACGTCGTTTTCTACAAATTTGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATAc                                                                                                                >  2:672712/1‑140 (MQ=255)            tAGGCGAGATCACGTCGTTTTCTACAAATTTGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATAc                                                                                                                >  1:483997/1‑136 (MQ=255)            tAGGCGAGATCACGTCGTTTTCTACAAATTTGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATAc                                                                                                                <  2:483997/136‑1 (MQ=255)                      cACGTCGTTTTCTACAAATTTGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATACCCATTAATctctct                                                                                                  <  1:120270/140‑1 (MQ=255)                         gtcgtTTTCTACAAATTTGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCa                                                                                               >  2:333271/1‑140 (MQ=255)                                 cTACAAATTTGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGt                                                                                       <  2:834618/140‑1 (MQ=255)                                   aCAAATTTGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGa                                                                                     >  2:854036/1‑140 (MQ=255)                                             tCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCAATgaga                                                                           <  2:19044/140‑1 (MQ=255)                                                     aTCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCAATGAGAGGcaacaa                                                                   <  1:854036/140‑1 (MQ=255)                                                     aTCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCAATGAGAGGcaacaa                                                                   <  2:749583/140‑1 (MQ=255)                                                                             accaTAAATCAATGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCAATGAGAGGCAACAAGAATGAACAATCGATCGGCCGTTa                                           <  1:672712/140‑1 (MQ=255)                                                                             accaTAAATCAATGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCAATGAGAGGCAACAAGAATGAACAATCGATCGGCCGTTa                                           <  1:52242/140‑1 (MQ=255)                                                                                         tGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCAATGAGAGGCAACAAGAATGAACAATCGATCGGCCGTTAGAATACTACGGt                               >  2:75318/1‑140 (MQ=255)                                                                                                   gTTGTAAAAATTGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCAATGAGAGGCAACAAGAATGAACAATCGATCGGCCGTTAGAATACTACGGTTACCAGCGGt                     >  1:374347/1‑140 (MQ=255)                                                                                                     tGTAAAAATTGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCAATGAGAGGCAACAAGAATGAACAATCGATCGGCCGTTAGAATACTACGGTTACCAGCGGTTa                   >  2:468258/1‑140 (MQ=255)                                                                                                       tAAAAATTGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCAATGAGAGGCAACAAGAATGAACAATCGATCGGCCGTTAGAATACTACGGTTACCAGCGGTTATc                 >  2:904566/1‑140 (MQ=255)                                                                                                              tGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCAATGAGAGGCAACAAGAATGAACAATCGATCGGCCGTTAGAATACTACGGTTACCAGCGGTTATCCaaaaaa          >  2:267818/1‑140 (MQ=255)                                                                                                              tGTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCAATGAGAGGCAACAAGAATGAACAATCGATCGGCCGTTAGAATACTACGGTTACCAGCGGTTATCCaaaaaa          <  1:333271/140‑1 (MQ=255)                                                                                                               gTATTTTTGGTACATGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCAATGAGAGGCAACAAGAATGAACAATCGATCGGCCGTTAGAATACTACGGTTACCAGCGGTTATCCAAAAAAc         <  2:814865/140‑1 (MQ=255)                                                                                                                      tGGTACATGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCAATGAGAGGCAACAAGAATGAACAATCGATCGGCCGTTAGAATACTACGGTTACCAGCGGTTATCCAAAAAACAGGTATg  <  1:75318/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                  CGGACATGTGATAGGCGAGATCACGTCGTTTTCTACAAATTTGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGGTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCAATGAGAGGCAACAAGAATGAACAATCGATCGGCCGTTAGAATACTACGGTTACCAGCGGTTATCCAAAAAACAGGTATG  >  NC_000913/2758450‑2758706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑