Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,204,045	G→T	G29G (GGC→GGA)	bacA ←	undecaprenyl pyrophosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,204,045	0	G	T	100.0%	181.1 / NA	53	G29G (GGC→GGA)	bacA	undecaprenyl pyrophosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/25);  total (28/25)

TGCCAATCAGGCCAAACAGACGCCGCCAGAACATCACTACTACCGCCAGAATTGATCCTAACTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGT  >  NC_000913/3203912‑3204181                                                                                                                                      |                                                                                                                                         tGCCAATCAGGCCAAACAGACGCCGCCAGAACATCACTACTACCGCCAGAATTGATCCTAACTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTg                                                                                                                                    >  1:121487/1‑140 (MQ=255)     aaTCAGGCCAAACAGACGCCGCCAGAACATCACTACTACCGCCAGAATTGATCCTAACTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGAAAATAATCATATGTCCCGTGCTgg                                                                                                                                >  2:724601/1‑140 (MQ=255)       tCAGGCCAAACAGACGCCGCCAGAACATCACTACTACCGCCAGAATTGATCCTAACTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGAt                                                                                                                              >  2:160151/1‑140 (MQ=255)                aCAGACGCCGCCAGAACATCACTACTACCGCCAGAATTGATCCTAACTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGa                                                                                                                     >  1:411430/1‑140 (MQ=255)                 cAGACGCCGCCAGAACATCACTACTACCGCCAGAATTGATCCTAACTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGaa                                                                                                                    <  2:497961/140‑1 (MQ=255)                          ccAGAACATCACTACTACCGCCAGAATTGATCCTAACTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCa                                                                                                           <  1:160151/140‑1 (MQ=255)                                cATCACTACTACCGCCAGAATTGATCCTAACTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCtt                                                                                                     <  1:382922/140‑1 (MQ=255)                                          acCGCCAGAATTGATCCTAACTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACAcc                                                                                           >  2:120107/1‑140 (MQ=255)                                             gCCAGAATTGATCCTAACTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCaa                                                                                        >  1:563552/1‑140 (MQ=255)                                              ccAGAATTGATCCTAACTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAat                                                                                       >  2:380282/1‑140 (MQ=255)                                                aGAATTGATCCTAACTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGAcc                                                                                                >  2:65061/1‑129 (MQ=255)                                                aGAATTGATCCTAACTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGAcc                                                                                                <  1:65061/129‑1 (MQ=255)                                                   aTTGATCCTAACTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGc                                                                                  >  1:130308/1‑140 (MQ=255)                                                         ccTAACTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTAt                                                                            >  1:388611/1‑140 (MQ=255)                                                            aaCTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATcag                                                                         >  2:454646/1‑140 (MQ=255)                                                            aaCTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATcag                                                                         >  1:784516/1‑140 (MQ=255)                                                             aCTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATcagc                                                                        >  1:714858/1‑140 (MQ=255)                                                             aCTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATcagc                                                                        >  1:909207/1‑140 (MQ=255)                                                              cTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATcagca                                                                       <  2:411430/140‑1 (MQ=255)                                                               tGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATcagcag                                                                      <  1:380282/140‑1 (MQ=255)                                                                 gATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCg                                                                    >  1:409836/1‑140 (MQ=255)                                                                 gATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCg                                                                    >  2:345840/1‑140 (MQ=255)                                                                 gATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCg                                                                    >  2:407498/1‑140 (MQ=255)                                                                  aTCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATca                                                                          >  1:132236/1‑133 (MQ=255)                                                                  aTCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATca                                                                          <  2:132236/133‑1 (MQ=255)                                                                  aTCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGa                                                                   <  2:407489/140‑1 (MQ=255)                                                                  aTCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGa                                                                   >  2:3762/1‑140 (MQ=255)                                                                  aTCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGa                                                                   >  2:371327/1‑140 (MQ=255)                                                                    cacaACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGt                                                                 <  1:120107/140‑1 (MQ=255)                                                                      caACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGc                                                               >  2:284955/1‑140 (MQ=255)                                                                         cTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCata                                                            >  2:724697/1‑140 (MQ=255)                                                                         cTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCata                                                            >  2:357494/1‑140 (MQ=255)                                                                          ttCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCatat                                                           <  2:515384/140‑1 (MQ=255)                                                                          ttCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCatat                                                           <  2:427773/140‑1 (MQ=255)                                                                            cAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCg                                                         <  1:609431/140‑1 (MQ=255)                                                                               aGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCtc                                                      <  2:784516/140‑1 (MQ=255)                                                                                    ttCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCaa                                                 >  1:2537/1‑140 (MQ=255)                                                                                      cGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATa                                               <  1:724697/140‑1 (MQ=255)                                                                                      cGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATa                                               <  1:546758/140‑1 (MQ=255)                                                                                            gtCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCt                                                       >  2:553435/1‑126 (MQ=255)                                                                                            gtCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCt                                                       <  1:553435/126‑1 (MQ=255)                                                                                            gtCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAAcccc                                         >  1:517996/1‑140 (MQ=255)                                                                                              cGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAGCCCCTa                                       <  1:323166/140‑1 (MQ=255)                                                                                                   tCAAACCCCAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAatta                                  <  2:130308/140‑1 (MQ=255)                                                                                                        ccccAACAAGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTaaa                             <  2:582379/140‑1 (MQ=255)                                                                                                            aacaaGTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGt                         >  1:243991/1‑140 (MQ=255)                                                                                                                 gTGACCGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGc                    >  2:362091/1‑140 (MQ=255)                                                                                                                     ccGACAATAATCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGt                <  2:909207/140‑1 (MQ=255)                                                                                                                          aataatCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGaa           <  2:388611/140‑1 (MQ=255)                                                                                                                             aatCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAacta        <  1:371327/140‑1 (MQ=255)                                                                                                                              atCATATGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAactac       <  1:724601/140‑1 (MQ=255)                                                                                                                               tCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATGTTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAactact      <  2:518052/140‑1 (MQ=255)                                                                                                                                   atGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGt  <  2:2537/140‑1 (MQ=255)                                                                                                                                   atGTCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGt  <  2:121487/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TGCCAATCAGGCCAAACAGACGCCGCCAGAACATCACTACTACCGCCAGAATTGATCCTAACTGGATCACAACTTCAAAGGTTTTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCGT  >  NC_000913/3203912‑3204181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑