Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,778,459	T→C	V354A (GTC→GCC)	lldP →	lactate/glycolate:H(+) symporter LldP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,778,459	0	T	C	100.0%	175.3 / NA	46	V354A (GTC→GCC)	lldP	lactate/glycolate:H(+) symporter LldP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/7);  total (39/7)

CCTGACAGCTACCGTAACACTGTGGAGTATCCCGCCGTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGC  >  NC_000913/3778322‑3778586                                                                                                                                           |                                                                                                                                cctgGCAGCTACCGTAACACTGTGGAGTATCCCGCCGTTTAAAGCCCTGTTCGCATAGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCa                                                                                                                               >  2:357742/1‑140 (MQ=255)  ctgACAGCTACCGTAACACTGTGGAGTATCCCGCCGTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAg                                                                                                                              <  1:616194/140‑1 (MQ=255)    gACAGCTACCGTAACACTGTGGAGTATCCCGCCGTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCg                                                                                                                            >  1:648443/1‑140 (MQ=255)    gACAGCTACCGTAACACTGTGGAGTATCCCGCCGTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCg                                                                                                                            <  2:735204/140‑1 (MQ=255)    gACAGCTACCGTAACACTGTGGAGTATCCCGCCGTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCg                                                                                                                            >  2:677758/1‑140 (MQ=255)       aGCTACCGTAACACTGTGGAGTATCCCGCCGTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAgg                                                                                                                         <  1:378968/140‑1 (MQ=255)            ccGTAACACTGTGGAGTATCCCGCCGTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACa                                                                                                                    >  1:853338/1‑140 (MQ=255)                      gtgGAGTATCCCGCCGTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATgccg                                                                                                          <  2:372200/140‑1 (MQ=255)                       tgGAGTATCCCGCCGTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATgccgc                                                                                                         >  1:93456/1‑140 (MQ=255)                       tgGAGTATCCCGCCGTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATgccgc                                                                                                         >  2:406636/1‑140 (MQ=255)                              tCCCGCCGTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGttt                                                                                                  <  1:487029/140‑1 (MQ=255)                               cccGCCGTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTa                                                                                                 >  2:110677/1‑140 (MQ=255)                               cccGCCGTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTa                                                                                                 >  1:464786/1‑140 (MQ=255)                               cccGCCGTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTa                                                                                                 >  1:757141/1‑140 (MQ=255)                                 cgccgTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAg                                                                                               >  1:399140/1‑140 (MQ=255)                                 cgccgTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAg                                                                                               >  2:633925/1‑140 (MQ=255)                                 cgccgTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAg                                                                                               >  1:172302/1‑140 (MQ=255)                                 cgccgTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAg                                                                                               >  1:107570/1‑140 (MQ=255)                                  gccgTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGt                                                                                              <  2:441859/140‑1 (MQ=255)                                    cgTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGttt                                                                                            >  1:806371/1‑140 (MQ=255)                                                       aTCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCa                                                                            <  2:116210/137‑1 (MQ=255)                                                       aTCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCa                                                                            >  1:116210/1‑137 (MQ=255)                                                       aTCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCg                                                                         >  1:665337/1‑140 (MQ=255)                                                       aTCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCg                                                                         >  1:823410/1‑140 (MQ=255)                                                            gTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCAcc                                                                    >  2:538198/1‑140 (MQ=255)                                                                    cTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCt                                                            >  2:763287/1‑140 (MQ=255)                                                                         ggggggggTGATCAATATCCCGGTGCCGTCCCTCGATAACCTGGTTGCCC‑GTATGCCGCCAGTGCCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCCCCGGTACCGCCATTCTGTTtg                                                       <  2:806371/135‑1 (MQ=255)                                                                               ggTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCAc                                                 >  2:85748/1‑140 (MQ=255)                                                                                 tGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTg                                               >  2:762212/1‑140 (MQ=255)                                                                                   gtaaaTATCCCGGGGCCGTCCCTCAAAAAAC‑GGTTCCCCTGTACGCCGCCAGGGGCCTGCGAGACTACAGCTTATCCCGCCGTGTTTAAGTTTGTCTGTTTCTCTGCCCCCGGCACCGCCATTCTGTTTGCTGCACTGct                                             <  2:805259/137‑1 (MQ=255)                                                                                           tCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTg                                     >  1:687128/1‑140 (MQ=255)                                                                                            ccGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGt                                    >  2:386155/1‑140 (MQ=255)                                                                                             cGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTc                                   >  1:750191/1‑140 (MQ=255)                                                                                               gTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCtc                                 >  1:805259/1‑139 (MQ=255)                                                                                                tGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTgg                                >  1:705487/1‑140 (MQ=255)                                                                                                  ccGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCt                              >  1:304669/1‑140 (MQ=255)                                                                                                  ccGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCt                              >  1:650505/1‑140 (MQ=255)                                                                                                  ccGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCt                              >  1:531470/1‑140 (MQ=255)                                                                                                            aTAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGaaa                    >  1:881691/1‑140 (MQ=255)                                                                                                            aTAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGaaa                    >  2:520832/1‑140 (MQ=255)                                                                                                            aTAAACTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGaaa                    >  2:579165/1‑140 (MQ=255)                                                                                                                aCTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGt                <  1:138073/140‑1 (MQ=255)                                                                                                                aCTGGTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGt                >  2:138073/1‑140 (MQ=255)                                                                                                                    tttGCCC‑GTATGCCGCCGGGGGCCAGCGGGCCTACAGCCTATGCCGCCGTGTTTAAGTTTGCCTGGTTCTCTGCCCCCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGa            <  2:464786/139‑1 (MQ=255)                                                                                                                    gTTGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGa            >  1:353151/1‑140 (MQ=255)                                                                                                                     ttcccc‑GTAGCCCGCCGGGGGCCGGCGAGGCTACACCCTCTGCCGCGGTGTTTAATTTTGACTGGTTCTCGGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGAc           <  2:203140/137‑1 (MQ=255)                                                                                                                     ttGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGAc           >  1:203140/1‑140 (MQ=255)                                                                                                                      tGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACg          >  1:419370/1‑140 (MQ=255)                                                                                                                      tGCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACg          >  1:826431/1‑140 (MQ=255)                                                                                                                       gCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGc         >  1:313638/1‑140 (MQ=255)                                                                                                                       gCCC‑GTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGc         >  1:588078/1‑140 (MQ=255)                                                                                                                              ttGCCGCCGGGGGCCAGGGGGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAg   <  2:521752/139‑1 (MQ=255)                                                                                                                               tGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGc  >  1:903511/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                CCTGACAGCTACCGTAACACTGTGGAGTATCCCGCCGTTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCC‑GTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGC  >  NC_000913/3778322‑3778586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑