Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,381,952	C→A	E123* (GAG→TAG)	frdA ←	fumarate reductase flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,381,952	0	C	A	100.0%	164.9 / NA	47	E123* (GAG→TAG)	frdA	fumarate reductase flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/23);  total (24/23)

ATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTCGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCAC  >  NC_000913/4381824‑4382086                                                                                                                                 |                                                                                                                                       atcatcAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATgc                                                                                                                             <  1:259569/140‑1 (MQ=255)   catcaACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATgccg                                                                                                                           <  2:789397/140‑1 (MQ=255)       aaCCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGCACCAGGTGCGCTAGATTTTCATGCCGCCGa                                                                                                                       >  1:700008/1‑140 (MQ=255)       aaCCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGa                                                                                                                       <  2:700008/140‑1 (MQ=255)         ccAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAg                                                                                                                     >  2:467376/1‑140 (MQ=255)         ccAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAg                                                                                                                     >  1:225534/1‑140 (MQ=255)          cAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGc                                                                                                                    >  2:577988/1‑140 (MQ=255)          cAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGc                                                                                                                    >  2:700663/1‑140 (MQ=255)             aaTATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGac                                                                                                                 >  2:303627/1‑140 (MQ=255)                  ccAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGacgtacg                                                                                                            <  2:656175/140‑1 (MQ=255)                      cACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGa                                                                                                        <  1:577988/140‑1 (MQ=255)                      cACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGa                                                                                                        <  2:160679/140‑1 (MQ=255)                       aCGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGAc                                                                                                       >  2:767139/1‑140 (MQ=255)                                     aaaaCGCTGGATCCGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGc                                                                                         <  2:546013/140‑1 (MQ=255)                                        aCGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGAc                                                                                      <  2:582601/140‑1 (MQ=255)                                         cGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACg                                                                                     <  1:60011/140‑1 (MQ=255)                                                 cTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATg                                                                             <  1:201147/140‑1 (MQ=255)                                                   gCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATggg                                                                           <  1:544520/140‑1 (MQ=255)                                                   gCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATggg                                                                           <  1:700663/140‑1 (MQ=255)                                                          ttGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATcccc                                                                    <  1:467376/140‑1 (MQ=255)                                                                     tCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGt                                                         >  1:115031/1‑140 (MQ=255)                                                                       tGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCACGTGCGCTAGATTTTCATGCCGCCGAAGCGACGGACGTTGACGCTATCATCCGGGCGCCGGCTCCATGGGCATCCCCACAGTTCCAGTTg                                                       >  2:105878/1‑140 (MQ=255)                                                                              gCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCAtt                                                >  1:669642/1‑140 (MQ=255)                                                                              gCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCAtt                                                >  2:22016/1‑140 (MQ=255)                                                                               cGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTGCATGGGCATCCCCACAGTTCCAGTTGGGTCAttt                                               >  2:34744/1‑140 (MQ=255)                                                                               cGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCAttt                                               >  1:779013/1‑140 (MQ=255)                                                                               cGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCAttt                                               >  1:283571/1‑140 (MQ=255)                                                                               cGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCAttt                                               >  2:106539/1‑140 (MQ=255)                                                                               cGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCAttt                                               >  1:197097/1‑140 (MQ=255)                                                                               cGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCAttt                                               >  2:336493/1‑140 (MQ=255)                                                                                 tgtgCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCg                                             >  1:182175/1‑140 (MQ=255)                                                                                    gcagcaTATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGtt                                          <  1:794184/140‑1 (MQ=255)                                                                                       gcaTATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTggg                                       <  2:875922/140‑1 (MQ=255)                                                                                         atatGGAAGCCGGTCTTATCGGCGGCGAACCAGGGGCGCTAGATTTTTATGCCGCCCAAGCGACGTACGGTGACGCTACCATCCGGGCGACCGCTCCATGGGCATCCCCACAGTTCCAGTTTGGTCCTTTCGGTTGGGCa                                     >  2:21076/1‑140 (MQ=255)                                                                                                  ccGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGAc                            >  2:38214/1‑140 (MQ=255)                                                                                                  ccGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGAc                            >  1:231961/1‑140 (MQ=255)                                                                                                  ccGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGAc                            >  2:185553/1‑140 (MQ=255)                                                                                                  ccGGTCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGAc                            >  2:328911/1‑140 (MQ=255)                                                                                                      tCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGaaa                        >  2:638056/1‑140 (MQ=255)                                                                                                      tCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGaaa                        >  1:457102/1‑140 (MQ=255)                                                                                                      tCTTATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGaaa                        <  2:669642/140‑1 (MQ=255)                                                                                                        ttATCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAaata                      <  1:821297/140‑1 (MQ=255)                                                                                                          aTCGGCGGCGAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCGTCCCCACAGTTCCAGTTGGCTCATTTCGGTTGGGCAGTGGTGGACGAaataat                    <  2:649003/140‑1 (MQ=255)                                                                                                               cggcgAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACg               <  1:38214/140‑1 (MQ=255)                                                                                                               cggcgAACCAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACg               <  2:779013/140‑1 (MQ=255)                                                                                                                ggcgAACCAGGTGCGCTAGATTTTCATGCCGCCGAAACGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGGTGGGCAGTGGCGGACGAAATAATCCACGa              >  2:196074/1‑140 (MQ=255)                                                                                                                       cAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTg       <  1:638056/140‑1 (MQ=255)                                                                                                                       cAGGTGCGCTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTg       <  1:34744/140‑1 (MQ=255)                                                                                                                            gcgcTAGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTcac  <  2:225534/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                       ATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTCGATTTTCATGCCGCCGAAGCGACGTACGTTGACGCTACCATCCGGGCGACGGCTCCATGGGCATCCCCACAGTTCCAGTTGGGTCATTTCGGTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCAC  >  NC_000913/4381824‑4382086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑