Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	492,598	C→A	R169R (CGC→CGA)  R169R (CGC→CGA)	dnaX → dnaX →	DNA polymerase III subunit tau DNA polymerase III subunit tau

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	492,598	0	C	A	100.0%	137.8 / NA	40	R169R (CGC→CGA)  R169R (CGC→CGA)	dnaX dnaX	DNA polymerase III subunit tau DNA polymerase III subunit tau
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/19);  total (21/19)

CGACGAAGTGCATATGCTGTCGCGCCACAGCTTTAACGCACTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGCTGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCC  >  NC_000913/492466‑492731                                                                                                                                     |                                                                                                                                      cgacgaAGTGCATATGCTGTCGCGCCACAGCTTTAACGCACTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGc                                                                                                                                >  2:532447/1‑140 (MQ=255)           cATATGCTGTCGCGCCACAGCTTTAACGCACTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCAtct                                                                                                                      >  1:127618/1‑140 (MQ=255)             tatGCTGTCGCGCCACAGCTTTAACGCACTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCa                                                                                                                    <  1:768242/140‑1 (MQ=255)                   gTCGCGCCACAGCTTTAACGCACTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAg                                                                                                                  <  2:125824/136‑1 (MQ=255)                   gTCGCGCCACAGCTTTAACGCACTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAg                                                                                                                  >  1:125824/1‑136 (MQ=255)                   gTCGCGCCACAGCTTTAACGCACTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGgcgc                                                                                                              >  2:81547/1‑140 (MQ=255)                   gTCGCGCCACAGCTTTAACGCACTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGgcgc                                                                                                              >  2:258017/1‑140 (MQ=255)                   gTCGCGCCACAGCTTTAACGCACTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGgcgc                                                                                                              >  1:618170/1‑140 (MQ=255)                   gTCGCGCCACAGCTTTAACGCACTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGgcgc                                                                                                              <  1:549117/140‑1 (MQ=255)                    tCGCGCCACAGCTTTAACGCACTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCt                                                                                                             <  1:581690/140‑1 (MQ=255)                         ccACAGCTTTAACGCACTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATg                                                                                                        >  1:633828/1‑140 (MQ=255)                                    aCGCACTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAAtt                                                                                             <  2:214711/140‑1 (MQ=255)                                    aCGCACTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAAtt                                                                                             >  1:34706/1‑140 (MQ=255)                                    aCGCACTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAAtt                                                                                             <  1:258017/140‑1 (MQ=255)                                        aCTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGcc                                                                                         >  1:778942/1‑140 (MQ=255)                                         cTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCa                                                                                        <  1:425763/140‑1 (MQ=255)                                           gTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATc                                                                                      <  1:81547/140‑1 (MQ=255)                                                    ccTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGc                                                                             <  2:34706/140‑1 (MQ=255)                                                      ttGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAg                                                                              >  2:368245/1‑137 (MQ=255)                                                      ttGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAg                                                                              <  1:368245/137‑1 (MQ=255)                                                      ttGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGcac                                                                           >  1:513279/1‑140 (MQ=255)                                                             gccgccGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCa                                                                    >  1:499391/1‑140 (MQ=255)                                                              ccgccgGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCaa                                                                   >  2:742846/1‑140 (MQ=255)                                                               cgccgGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAAc                                                                  <  1:839661/140‑1 (MQ=255)                                                               cgccgGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAAc                                                                  <  2:127618/140‑1 (MQ=255)                                                                 ccgGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAACga                                                                >  1:210736/1‑140 (MQ=255)                                                                            tAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAACGAAGAACATATCg                                                     >  1:291109/1‑140 (MQ=255)                                                                                 ttCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAACGAAGAACATATCGCTCAc                                                <  2:633828/140‑1 (MQ=255)                                                                                   ctgctgGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAACGAAGAACATATCGCTCACGa                                              >  2:136716/1‑140 (MQ=255)                                                                                    tgctgGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAACGAAGAACATATCGCTCACGAg                                             >  1:274709/1‑140 (MQ=255)                                                                                        ggCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAACGAAGAACATATCGCTCACGAGCcgc                                         >  1:559766/1‑140 (MQ=255)                                                                                         gCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAACGAAGAACATATCGCTCACGAGCcgcg                                        <  2:778942/140‑1 (MQ=255)                                                                                                              ttGCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCAcg                   >  1:886212/1‑140 (MQ=255)                                                                                                                gCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCAcgcg                 <  1:404509/140‑1 (MQ=255)                                                                                                                gCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCAcgcg                 <  2:559766/140‑1 (MQ=255)                                                                                                                gCCGGTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCAcgcg                 <  2:618170/140‑1 (MQ=255)                                                                                                                    gTGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGcgccgc             <  1:279806/140‑1 (MQ=255)                                                                                                                     tGACGATTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCt            >  1:838241/1‑140 (MQ=255)                                                                                                                          aTTTTGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAgg       <  1:532447/140‑1 (MQ=255)                                                                                                                            tttGTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCa     <  2:619338/140‑1 (MQ=255)                                                                                                                               gTCACGATGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGcc  >  1:526401/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      CGACGAAGTGCATATGCTGTCGCGCCACAGCTTTAACGCACTGTTAAAAACCCTTGAAGAGCCGCCGGAGCACGTTAAGTTTCTGCTGGCGACGACCGATCCACAGAAATTGCCGGTGACGATTTTGTCACGCTGTCTGCAATTTCATCTCAAGGCGCTGGATGTCGAGCAAATTCGCCATCAGCTTGAGCACATCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCC  >  NC_000913/492466‑492731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑