Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	553,788	A→G	D51D (GAT→GAC)	lpxH ←	UDP‑2,3‑diacylglucosamine diphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	553,788	0	A	G	100.0%	181.1 / NA	48	D51D (GAT→GAC)	lpxH	UDP‑2,3‑diacylglucosamine diphosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/28);  total (20/28)

TAACGTCATGCCACTTTCACGGGCAAAGCGTTTGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCA  >  NC_000913/553653‑553923                                                                                                                                        |                                                                                                                                        tAACGTCATGCCACTTTCACGGGCAAAGCGTTTGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGtcgt                                                                                                                                     <  2:656100/140‑1 (MQ=255)      tCATGCCACTTTCACGGGCAAAGCGTTTGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGtcgtcgtcg                                                                                                                                >  2:813921/1‑140 (MQ=255)             aCTTTCACGGGCAAAGCGTTTGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATcc                                                                                                                         <  2:88482/140‑1 (MQ=255)             aCTTTCACGGGCAAAGCGTTTGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATcc                                                                                                                         <  1:328464/140‑1 (MQ=255)               tttCACGGGCAAAGCGTTTGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATcc                                                                                                                         <  2:467625/138‑1 (MQ=255)               tttCACGGGCAAAGCGTTTGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATcc                                                                                                                         >  1:467625/1‑138 (MQ=255)               tttCACGGGCAAAGCGTTTGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCAt                                                                                                                       >  2:214309/1‑140 (MQ=255)               tttCACGGGCAAAGCGTTTGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCAt                                                                                                                       <  1:525813/140‑1 (MQ=255)               tttCACGGGCAAAGCGTTTGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCAt                                                                                                                       >  2:544581/1‑140 (MQ=255)                 tCACGGGCAAAGCGTTTGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGc                                                                                                                     >  2:153680/1‑140 (MQ=255)                   aCGGGCAAAGCGTTTGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCTGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCtt                                                                                                                   >  2:461436/1‑140 (MQ=255)                          aaGCGTTTGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAg                                                                                                            <  1:153680/140‑1 (MQ=255)                           aGCGTTTGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATcc                                                                                                                         >  2:374003/1‑126 (MQ=255)                           aGCGTTTGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATcc                                                                                                                         <  1:374003/126‑1 (MQ=255)                               tttGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCTGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGc                                                                                                       <  1:461436/140‑1 (MQ=255)                               tttGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGc                                                                                                       <  1:60843/140‑1 (MQ=255)                               tttGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGc                                                                                                       <  1:361005/140‑1 (MQ=255)                               tttGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGc                                                                                                       <  2:383183/140‑1 (MQ=255)                               tttGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGc                                                                                                       <  2:675861/140‑1 (MQ=255)                               tttGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGc                                                                                                       <  1:214309/140‑1 (MQ=255)                               tttGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGc                                                                                                       <  1:914536/140‑1 (MQ=255)                                 tGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGc                                                                                                       <  2:182899/138‑1 (MQ=255)                                 tGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGc                                                                                                       >  1:182899/1‑138 (MQ=255)                                 tGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCa                                                                                                     <  1:417376/140‑1 (MQ=255)                                                                  aaTAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTcc                                                                    >  2:893604/1‑140 (MQ=255)                                                                       cAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGc                                                               >  2:136828/1‑140 (MQ=255)                                                                       cAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGc                                                               >  2:407139/1‑140 (MQ=255)                                                                         gggAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTa                                                             >  2:181958/1‑140 (MQ=255)                                                                                 cGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGc                                                     >  1:84305/1‑140 (MQ=255)                                                                                       cGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGaaaa                                               <  1:515008/140‑1 (MQ=255)                                                                                             ccGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGACGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCCCCCCCCGCTAAAAAACGCAGAAAACcggcg                                         >  2:659961/1‑140 (MQ=255)                                                                                                cTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTg                                      >  2:545291/1‑140 (MQ=255)                                                                                                   tGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATc                                   <  2:532066/140‑1 (MQ=255)                                                                                                     aTCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGc                                 <  1:813921/140‑1 (MQ=255)                                                                                                     aTCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGc                                 <  1:407139/140‑1 (MQ=255)                                                                                                        gccgccgccATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTCCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCgg                              <  1:659961/140‑1 (MQ=255)                                                                                                                cATCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCa                      <  1:707409/140‑1 (MQ=255)                                                                                                                  tCTTGCGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACg                    <  1:181958/140‑1 (MQ=255)                                                                                                                       cGATGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCagag               >  1:225642/1‑140 (MQ=255)                                                                                                                          tGGAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATg            <  2:89210/140‑1 (MQ=255)                                                                                                                            gAGTGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGaa          >  1:57241/1‑140 (MQ=255)                                                                                                                               tGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCag                 <  1:212697/130‑1 (MQ=255)                                                                                                                               tGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCag                 >  2:212697/1‑130 (MQ=255)                                                                                                                               tGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGAt       <  1:893604/140‑1 (MQ=255)                                                                                                                               tGGGTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGAt       <  2:84305/140‑1 (MQ=255)                                                                                                                                 ggTTGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCt     <  1:761800/140‑1 (MQ=255)                                                                                                                                    tGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCa  >  1:755035/1‑140 (MQ=255)                                                                                                                                    tGGGGTCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCa  >  1:113547/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        TAACGTCATGCCACTTTCACGGGCAAAGCGTTTGCCGAGCAGAAAATCACGGTTGCCATGAATGAAATAACAGGGAACGCCGGAATCGGACACCGCTTTGATCGCCGCCGCCATCTTGCGATGGAGTGGGTTGGGATCGTCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCA  >  NC_000913/553653‑553923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑