Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,997,889	C→A	S236* (TCG→TAG)	yigB →	5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,997,889	0	C	A	100.0%	335.0 / NA	94	S236* (TCG→TAG)	yigB	5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (40/54);  total (40/54)

CTGACGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTCGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAAC  >  NC_000913/3997757‑3998022                                                                                                                                     |                                                                                                                                      cTGACGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGata                                                                                                                                >  1:366954/1‑140 (MQ=255)  tGACGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGatat                                                                                                                               <  1:1092343/140‑1 (MQ=255)  tGACGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGatat                                                                                                                               >  2:1467395/1‑140 (MQ=255)   gACGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGAtata                                                                                                                              >  1:1497037/1‑140 (MQ=255)   gACGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGAAGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGAtata                                                                                                                              >  2:1482469/1‑140 (MQ=255)    aCGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATaa                                                                                                                             >  2:880219/1‑140 (MQ=255)    aCGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATaa                                                                                                                             >  2:1245153/1‑140 (MQ=255)    aCGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTAGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATaa                                                                                                                             >  1:873366/1‑140 (MQ=255)     cGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAAt                                                                                                                            <  2:1222900/140‑1 (MQ=255)        gggtgggGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAg                                                                                                                         <  1:689840/140‑1 (MQ=255)        gggtgggGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAAATGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAg                                                                                                                         <  1:403510/140‑1 (MQ=255)         ggtgggGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGAtata                                                                                                                              >  1:1030268/1‑134 (MQ=255)         ggtgggGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGAtata                                                                                                                              <  2:1030268/134‑1 (MQ=255)         ggtgggGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGc                                                                                                                        >  2:903182/1‑140 (MQ=255)         ggtgggGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGc                                                                                                                        >  2:479505/1‑140 (MQ=255)         ggtgggGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGc                                                                                                                        <  2:530149/140‑1 (MQ=255)         ggtgggGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGc                                                                                                                        >  1:651285/1‑140 (MQ=255)         ggtgggGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGc                                                                                                                        >  1:425082/1‑140 (MQ=255)          gtgggGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCa                                                                                                                       <  1:1107393/140‑1 (MQ=255)          gtgggGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCa                                                                                                                       <  2:1316365/140‑1 (MQ=255)          gtgggGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCa                                                                                                                       <  2:366954/140‑1 (MQ=255)          gtgggGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCa                                                                                                                       <  1:1208750/140‑1 (MQ=255)           tgggGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCaa                                                                                                                      <  1:1311606/140‑1 (MQ=255)            ggggCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCaaa                                                                                                                     <  2:207229/140‑1 (MQ=255)             gggCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAAt                                                                                                                    <  1:261009/140‑1 (MQ=255)             gggCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCAACTCTGACCTAGCTGATATAATCAGCAAAt                                                                                                                    <  2:1548602/140‑1 (MQ=255)              ggCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCa                                                                                                                       <  2:381814/136‑1 (MQ=255)              ggCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCa                                                                                                                       >  1:381814/1‑136 (MQ=255)              ggCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATc                                                                                                                   >  2:1455840/1‑140 (MQ=255)              ggCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATc                                                                                                                   <  2:395219/140‑1 (MQ=255)              ggCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATc                                                                                                                   <  1:1292114/140‑1 (MQ=255)              ggCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATc                                                                                                                   <  1:1532402/140‑1 (MQ=255)              ggCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATc                                                                                                                   <  1:226471/140‑1 (MQ=255)              ggCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATc                                                                                                                   <  2:19569/140‑1 (MQ=255)              ggCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATc                                                                                                                   <  1:1158359/140‑1 (MQ=255)              ggCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATc                                                                                                                   <  1:1245153/140‑1 (MQ=255)              ggCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATc                                                                                                                   <  1:1278471/140‑1 (MQ=255)              ggCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATc                                                                                                                   <  1:454932/140‑1 (MQ=255)              ggCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTAGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATc                                                                                                                   <  2:873366/140‑1 (MQ=255)              ggCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGAAGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATc                                                                                                                   <  1:1482469/140‑1 (MQ=255)               gCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCt                                                                                                                  <  2:1511447/140‑1 (MQ=255)                   ttCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGtat                                                                                                              >  1:794458/1‑140 (MQ=255)                   ttCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGtat                                                                                                              >  2:1547946/1‑140 (MQ=255)                    tCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGtata                                                                                                             >  2:660092/1‑140 (MQ=255)                        aGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGtatatata                                                                                                         >  2:464292/1‑140 (MQ=255)                         gCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATAc                                                                                                        >  1:422232/1‑140 (MQ=255)                          cGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATAcc                                                                                                       <  1:955297/140‑1 (MQ=255)                          cGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATAcc                                                                                                       <  1:880219/140‑1 (MQ=255)                           ggAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATAccc                                                                                                      <  1:1455840/140‑1 (MQ=255)                           ggAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATAccc                                                                                                      <  2:651285/140‑1 (MQ=255)                            gAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCa                                                                                                     <  2:336271/140‑1 (MQ=255)                                 cAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCttt                                                                                                <  1:920014/140‑1 (MQ=255)                                    gCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTg                                                                                             >  2:194769/1‑140 (MQ=255)                                            gATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAggg                                                                                     <  2:1570980/140‑1 (MQ=255)                                              tCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCg                                                                                   <  2:824742/140‑1 (MQ=255)                                                  aCCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTgc                                                                               >  1:1205933/1‑140 (MQ=255)                                                  aCCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTgc                                                                               >  2:1428247/1‑140 (MQ=255)                                                   ccGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTgcg                                                                              <  2:1493325/140‑1 (MQ=255)                                                   ccGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTgcg                                                                              <  2:794458/140‑1 (MQ=255)                                                            ggCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGc                                                                     >  2:911785/1‑140 (MQ=255)                                                             gCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGcc                                                                    <  2:321235/140‑1 (MQ=255)                                                             gCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGcc                                                                    <  2:425082/140‑1 (MQ=255)                                                                       gCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTAttt                                                          >  2:1031020/1‑140 (MQ=255)                                                                          aagcTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTa                                                       >  2:1409383/4‑140 (MQ=255)                                                                           aCCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTAc                                                      <  1:1505933/140‑1 (MQ=255)                                                                               gggACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACgcgg                                                  >  2:920559/1‑140 (MQ=255)                                                                               gggACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACgcgg                                                  >  1:388585/1‑140 (MQ=255)                                                                                ggACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACgcggc                                                 >  2:1479434/1‑140 (MQ=255)                                                                                  aCAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGcggcgg                                               >  1:97452/1‑140 (MQ=255)                                                                                    aGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTg                                             >  2:445035/1‑140 (MQ=255)                                                                                     gCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTAcg                                                     <  2:1396159/131‑1 (MQ=255)                                                                                     gCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTAcg                                                     >  1:1396159/1‑131 (MQ=255)                                                                                     gCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGc                                            >  1:1274169/1‑140 (MQ=255)                                                                                     gCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGc                                            >  1:1213269/1‑140 (MQ=255)                                                                                       cGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCa                                          >  1:893331/1‑140 (MQ=255)                                                                                            aCTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGa                                     <  2:1497037/140‑1 (MQ=255)                                                                                            aCTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGa                                     <  1:479505/140‑1 (MQ=255)                                                                                            aCTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGa                                     <  2:698498/140‑1 (MQ=255)                                                                                            aCTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGa                                     <  1:1002858/140‑1 (MQ=255)                                                                                            aCTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGa                                     <  1:1167130/140‑1 (MQ=255)                                                                                            aCTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGa                                     <  2:388585/140‑1 (MQ=255)                                                                                                ccGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGtt                                 <  2:1213269/140‑1 (MQ=255)                                                                                                ccGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGtt                                 >  1:816047/1‑140 (MQ=255)                                                                                                ccGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGtt                                 >  2:1367956/1‑140 (MQ=255)                                                                                                     tCTGGAAATTTCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTa                            >  2:21416/1‑140 (MQ=255)                                                                                                              ttCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGa                   <  2:1523184/140‑1 (MQ=255)                                                                                                              ttCCCGGTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGa                   <  1:1367956/140‑1 (MQ=255)                                                                                                                   ggTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGcc              <  2:1259539/140‑1 (MQ=255)                                                                                                                    gTTGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCt             <  1:920559/140‑1 (MQ=255)                                                                                                                      tGGCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTa           <  2:1335635/140‑1 (MQ=255)                                                                                                                        gCATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAAt         <  2:816047/140‑1 (MQ=255)                                                                                                                         cATCTCTGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATg        >  2:1262342/1‑140 (MQ=255)                                                                                                                               tGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAAc  >  2:587489/1‑140 (MQ=255)                                                                                                                               tGACCTAGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAAc  >  1:1301020/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      CTGACGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTCGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAAC  >  NC_000913/3997757‑3998022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑