BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,223,453	C→G	A274G (GCC→GGC)	ebgA →	evolved beta‑D‑galactosidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,223,453	0	C	G	100.0%	483.8 / NA	126	A274G (GCC→GGC)	ebgA	evolved beta‑D‑galactosidase subunit alpha
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (61/65); total (61/65)

Only 100 of 126 total aligned reads displayed.

GCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCG  >  NC_000913/3223316‑3223585

gCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCa                                                                                                                                    >  2:1001323/1‑140 (MQ=255)

  tttCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAgc                                                                                                                                  <  1:637729/140‑1 (MQ=255)

          gAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAgctttgcttt                                                                                                                          <  2:111964/140‑1 (MQ=255)

              tggtgCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACt                                                                                                                      >  2:562443/1‑140 (MQ=255)

              tggtgCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACt                                                                                                                      >  1:884810/1‑140 (MQ=255)

                  gCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCg                                                                                                                  <  1:1565741/140‑1 (MQ=255)

                   cTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGa                                                                                                                 >  2:305068/1‑140 (MQ=255)

                   cTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGa                                                                                                                 >  1:1504750/1‑140 (MQ=255)

                   cTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTATTACTGTCGa                                                                                                                 >  2:286576/1‑140 (MQ=255)

                    tGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGaa                                                                                                                <  2:1220810/140‑1 (MQ=255)

                        aaaTCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGc                                                                                                            >  2:997745/1‑140 (MQ=255)

                        aaaTCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGc                                                                                                            >  2:212163/1‑140 (MQ=255)

                          aTCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCg                                                                                                          <  1:562443/140‑1 (MQ=255)

                          aTCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCg                                                                                                          <  1:40753/140‑1 (MQ=255)

                             tcGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCgcag                                                                                                       <  1:852124/140‑1 (MQ=255)

                               gccgccTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCgcagca                                                                                                     <  2:653607/140‑1 (MQ=255)

                               gccgccTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCgcagca                                                                                                     <  2:867827/140‑1 (MQ=255)

                               gccgccTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCgcagca                                                                                                     <  1:1402726/140‑1 (MQ=255)

                               gccgccTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCgcagca                                                                                                     <  2:744873/140‑1 (MQ=255)

                               gccgccTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCgcagca                                                                                                     <  1:152437/140‑1 (MQ=255)

                               gccgccTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCgcagca                                                                                                     <  1:94956/140‑1 (MQ=255)

                                ccgccTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCaa                                                                                                    >  1:939151/1‑140 (MQ=255)

                                ccgccTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCaa                                                                                                    >  2:1405086/1‑140 (MQ=255)

                                ccgccTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCaa                                                                                                    >  1:19308/1‑140 (MQ=255)

                                ccgccTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCaa                                                                                                    >  2:269506/1‑140 (MQ=255)

                                   ccTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATgg                                                                                                 >  1:1495545/1‑140 (MQ=255)

                                    cTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGt                                                                                                <  2:430796/140‑1 (MQ=255)

                                    cTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGt                                                                                                <  2:1499600/140‑1 (MQ=255)

                                     tCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTc                                                                                               >  1:1804785/1‑140 (MQ=255)

                                      ccccTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTca                                                                                              <  1:74000/140‑1 (MQ=255)

                                      ccccTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTca                                                                                              <  1:506857/140‑1 (MQ=255)

                                           gtcgtcACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGa                                                                                         <  2:64384/140‑1 (MQ=255)

                                           gtcgtcACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGa                                                                                         <  2:480638/140‑1 (MQ=255)

                                           gtcgtcACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGa                                                                                         <  1:1133855/140‑1 (MQ=255)

                                            tcgtcACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGaa                                                                                        <  2:1144358/140‑1 (MQ=255)

                                                 acgacgCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATcccc                                                                                   <  2:19308/140‑1 (MQ=255)

                                                 acgacgCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATcccc                                                                                   <  2:1238527/140‑1 (MQ=255)

                                                 acgacgCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATcccc                                                                                   >  2:219963/1‑140 (MQ=255)

                                                      gCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATc                                                                              >  1:649061/1‑140 (MQ=255)

                                                            atatACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAcc                                                                        <  1:1141661/140‑1 (MQ=255)

                                                              ataCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCAt                                                                      <  2:649061/140‑1 (MQ=255)

                                                               taCCCTATTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATc                                                                     <  1:269506/140‑1 (MQ=255)

                                                                 cccTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTg                                                                   >  1:1309965/1‑140 (MQ=255)

                                                                  ccTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTgg                                                                  >  2:1748164/1‑140 (MQ=255)

                                                                    tGTTTGATGGCGAACGCGTGGTGCACAGTAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTc                                                                >  1:739853/1‑140 (MQ=255)

                                                                    tGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCt                                                                    >  1:1402835/1‑136 (MQ=255)

                                                                    tGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCt                                                                    <  2:1402835/136‑1 (MQ=255)

                                                                    tGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTc                                                                >  2:1650818/1‑140 (MQ=255)

                                                                    tGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTc                                                                >  2:313574/1‑140 (MQ=255)

                                                                    tGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTc                                                                >  2:388126/1‑140 (MQ=255)

                                                                    tGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTc                                                                >  2:124125/1‑140 (MQ=255)

                                                                    tGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTc                                                                >  2:568966/1‑140 (MQ=255)

                                                                    tGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTc                                                                >  1:392904/1‑140 (MQ=255)

                                                                    tGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTc                                                                >  2:720914/1‑140 (MQ=255)

                                                                     gTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCa                                                               >  1:805561/1‑140 (MQ=255)

                                                                      tttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCAt                                                              <  2:1276997/140‑1 (MQ=255)

                                                                      tttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCAt                                                              <  2:1309965/140‑1 (MQ=255)

                                                                       ttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATg                                                             <  2:884810/140‑1 (MQ=255)

                                                                        tGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGa                                                            >  1:741990/1‑140 (MQ=255)

                                                                         gATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGAc                                                           >  1:153145/1‑140 (MQ=255)

                                                                           tGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGc                                                         >  2:610512/1‑140 (MQ=255)

                                                                                aaCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGaaa                                                    <  2:976404/140‑1 (MQ=255)

                                                                                             cagcagCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCa                                       >  2:1759028/1‑140 (MQ=255)

                                                                                             cagcagCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCa                                       <  1:1650818/140‑1 (MQ=255)

                                                                                              agcagcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCaa                                      <  1:1718228/140‑1 (MQ=255)

                                                                                              agcagcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCaa                                      <  1:1232933/140‑1 (MQ=255)

                                                                                                 agcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGt                                   <  2:1715761/140‑1 (MQ=255)

                                                                                                   cgcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGtt                                  >  1:1288540/1‑139 (MQ=255)

                                                                                                   cgcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGtt                                  <  2:1288540/139‑1 (MQ=255)

                                                                                                   cgcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTc                                 >  2:1784395/1‑140 (MQ=255)

                                                                                                   cgcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTc                                 >  2:959430/1‑140 (MQ=255)

                                                                                                   cgcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTc                                 >  2:1631551/1‑140 (MQ=255)

                                                                                                    gcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCt                                <  2:1595705/140‑1 (MQ=255)

                                                                                                     ccATTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTg                               >  2:1027925/1‑140 (MQ=255)

                                                                                                       aTTGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGa                             <  1:981521/140‑1 (MQ=255)

                                                                                                        ttGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCaa                                      <  1:908291/130‑1 (MQ=255)

                                                                                                        ttGATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCaa                                      >  2:908291/1‑130 (MQ=255)

                                                                                                          gATCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAgt                          <  1:1405086/140‑1 (MQ=255)

                                                                                                           aTCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAgtg                         <  2:770778/140‑1 (MQ=255)

                                                                                                           aTCATTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAgtg                         <  1:549169/140‑1 (MQ=255)

                                                                                                              aTTTGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAgtggtg                      >  2:844471/1‑140 (MQ=255)

                                                                                                                ttGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGcc                    >  2:1770426/1‑140 (MQ=255)

                                                                                                                ttGGCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGcc                    >  1:840564/1‑140 (MQ=255)

                                                                                                                   gCAATTGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCcaca                 <  1:1001323/140‑1 (MQ=255)

                                                                                                                       ttGAAAAACTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGAGCCACAAcgc             >  1:1283186/1‑140 (MQ=255)

                                                                                                                              aCTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCt      <  2:741990/140‑1 (MQ=255)

                                                                                                                              aCTGACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCt      <  1:610512/140‑1 (MQ=255)

                                                                                                                                 gACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTcc   >  2:551078/1‑140 (MQ=255)

                                                                                                                                 gACCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTcc   >  1:1893700/1‑140 (MQ=255)

                                                                                                                                  aCCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCg  <  1:1091030/140‑1 (MQ=255)

                                                                                                                                  aCCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCg  <  2:485015/140‑1 (MQ=255)

                                                                                                                                  aCCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCg  <  1:1389840/140‑1 (MQ=255)

                                                                                                                                  aCCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCg  <  1:844471/140‑1 (MQ=255)

                                                                                                                                  aCCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCg  >  1:392289/1‑140 (MQ=255)

                                                                                                                                  aCCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCg  >  2:883952/1‑140 (MQ=255)

                                                                                                                                  aCCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCg  <  1:1631551/140‑1 (MQ=255)

                                                                                                                                  aCCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCg  >  1:1546427/1‑140 (MQ=255)

                                                                                                                                  aCCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCg  <  1:1482761/140‑1 (MQ=255)

                                                                                                                                  aCCAGCGGCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCg  <  1:1470565/140‑1 (MQ=255)

                                                                                                                                  aCCAGCGGCAGCTTCGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCg  >  2:1207369/1‑140 (MQ=255)

GCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCG  >  NC_000913/3223316‑3223585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`