Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,681,120	C→A	R274L (CGG→CTG)	yhjJ ←	peptidase M16 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,681,120	0	C	A	100.0%	286.5 / NA	80	R274L (CGG→CTG)	yhjJ	peptidase M16 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (43/37);  total (43/37)

TGCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCG  >  NC_000913/3680986‑3681256                                                                                                                                       |                                                                                                                                         tGCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTcc                                                                                                                                     >  1:649093/1‑140 (MQ=255)  gCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCt                                                                                                                                    <  2:405135/140‑1 (MQ=255)      ttACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACg                                                                                                                                >  1:1663447/1‑140 (MQ=255)      ttACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACg                                                                                                                                >  1:88618/1‑140 (MQ=255)       tACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCTGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGc                                                                                                                               >  1:1738385/1‑140 (MQ=255)       tACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGc                                                                                                                               >  2:926425/1‑140 (MQ=255)       tACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGc                                                                                                                               >  2:1036379/1‑140 (MQ=255)       tACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGc                                                                                                                               >  1:1216697/1‑140 (MQ=255)       tACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGc                                                                                                                               >  1:501772/1‑140 (MQ=255)        aCTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCa                                                                                                                              <  2:750927/140‑1 (MQ=255)        aCTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCa                                                                                                                              <  2:584209/140‑1 (MQ=255)        aCTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCa                                                                                                                              <  2:1804415/140‑1 (MQ=255)               cTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCgtc                                                                                                                       <  1:1617468/140‑1 (MQ=255)               cTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCgtc                                                                                                                       <  2:1621914/140‑1 (MQ=255)               cTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCgtc                                                                                                                       <  1:1681561/140‑1 (MQ=255)               cTTAACGCTTGCGGAACAGGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCgtc                                                                                                                       <  2:1258098/140‑1 (MQ=255)                ttAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCgt                                                                                                                        <  2:1776551/138‑1 (MQ=255)                ttAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCgt                                                                                                                        >  1:1776551/1‑138 (MQ=255)                 tAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCgtcag                                                                                                                     <  2:312193/140‑1 (MQ=255)                 tAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCgtcag                                                                                                                     <  2:1395828/140‑1 (MQ=255)                  aaCGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCgtcagt                                                                                                                    >  1:1792744/1‑140 (MQ=255)                  aaCGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCgtcagt                                                                                                                    >  1:826168/1‑140 (MQ=255)                   aCGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCgtcagtc                                                                                                                   <  2:649093/140‑1 (MQ=255)                      cTTCCTAAACAGCCCAAAACAGCCCCTCGCGGGCCAGGCCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAAACGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCata                                                                                                                <  1:445582/140‑1 (MQ=255)                         gCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCataata                                                                                                             >  2:1758979/1‑140 (MQ=255)                         gCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCataata                                                                                                             <  1:994427/140‑1 (MQ=255)                               cATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCAcc                                                                                                       >  1:983114/1‑140 (MQ=255)                                aTGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCg                                                                                                      <  1:1758979/140‑1 (MQ=255)                                  gCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCt                                                                                                    >  1:974453/1‑140 (MQ=255)                                    cAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTc                                                                                                  >  2:1590819/1‑140 (MQ=255)                                       aaCAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGTCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcgc                                                                                               >  2:1855297/1‑140 (MQ=255)                                       aaCAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcgc                                                                                               >  1:1441552/1‑140 (MQ=255)                                       aaCAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcgc                                                                                               >  2:815287/1‑140 (MQ=255)                                         cAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcgcac                                                                                             >  2:267982/1‑140 (MQ=255)                                         cAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcgcac                                                                                             <  2:1799882/140‑1 (MQ=255)                                         cAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcgcac                                                                                             <  2:885379/140‑1 (MQ=255)                                          aGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcgcacg                                                                                            <  1:879622/140‑1 (MQ=255)                                            cgcCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcgcacgca                                                                                          >  1:953226/1‑140 (MQ=255)                                            cgcCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcgcacgca                                                                                          >  2:945720/1‑140 (MQ=255)                                            cgcCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcgcacgca                                                                                          >  2:1271995/1‑140 (MQ=255)                                                 cgcgGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGga                                                                                     >  2:867333/1‑140 (MQ=255)                                                   cgGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGgaga                                                                                   <  1:1321338/140‑1 (MQ=255)                                                      gCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAg                                                                                >  1:1301094/1‑140 (MQ=255)                                                      gCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAg                                                                                >  2:567978/1‑140 (MQ=255)                                                       ccAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGc                                                                               <  2:501772/140‑1 (MQ=255)                                                       ccAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGc                                                                               <  1:1590819/140‑1 (MQ=255)                                                            tCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCgg                                                                          >  1:1253193/1‑140 (MQ=255)                                                               gCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCAc                                                                       <  1:1036379/140‑1 (MQ=255)                                                                cACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCAcc                                                                      >  2:351673/1‑140 (MQ=255)                                                                cACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCAcc                                                                      >  2:1519323/1‑140 (MQ=255)                                                                cACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCAcc                                                                      >  1:1082365/1‑140 (MQ=255)                                                                   gCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGa                                                                   >  2:1596299/1‑140 (MQ=255)                                                                       gTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTg                                                               <  2:262347/140‑1 (MQ=255)                                                                        tAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTgg                                                              >  1:793444/1‑140 (MQ=255)                                                                                  gTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGt                                                    >  2:1457415/1‑140 (MQ=255)                                                                                  gTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGt                                                    <  2:1216697/140‑1 (MQ=255)                                                                                              cACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGt                                        <  1:1855297/140‑1 (MQ=255)                                                                                                    tCGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCa                                  <  2:1663447/140‑1 (MQ=255)                                                                                                     cGGCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCaa                                 >  1:524490/1‑140 (MQ=255)                                                                                                      ggCTGCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCaaa                                >  2:1269805/1‑140 (MQ=255)                                                                                                          gCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGtt                            <  2:557951/140‑1 (MQ=255)                                                                                                          gCCACGGCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGtt                            <  2:793444/140‑1 (MQ=255)                                                                                                           gtactgCGTATCCCACATTATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGttt                           <  1:464669/135‑1 (MQ=255)                                                                                                                gCGTATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGttt                      >  1:843280/1‑140 (MQ=255)                                                                                                                   tATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTAtt                   <  2:953226/140‑1 (MQ=255)                                                                                                                   tATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTAtt                   <  2:1082365/140‑1 (MQ=255)                                                                                                                   tATCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTAtt                   <  1:1271995/140‑1 (MQ=255)                                                                                                                     tCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTg                 >  2:400441/1‑140 (MQ=255)                                                                                                                     tCCCACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTg                 <  1:926425/140‑1 (MQ=255)                                                                                                                      cccACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTgg                >  1:1613627/1‑140 (MQ=255)                                                                                                                      cccACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTgg                >  2:943523/1‑140 (MQ=255)                                                                                                                       ccACATGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGt               <  2:1613627/140‑1 (MQ=255)                                                                                                                          caTGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGa            >  2:110082/1‑140 (MQ=255)                                                                                                                          caTGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGa            >  1:1713217/1‑140 (MQ=255)                                                                                                                          caTGATAGATAACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGa            <  1:1457415/140‑1 (MQ=255)                                                                                                                             gatagataACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACaa         >  1:1139938/1‑140 (MQ=255)                                                                                                                               tagataACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAAcc       <  1:815287/140‑1 (MQ=255)                                                                                                                               tagataACAGGTCCTGACGCACCGCGGCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAAcc       >  1:942476/1‑140 (MQ=255)                                                                                                                                  ataACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAg    >  2:1853339/1‑140 (MQ=255)                                                                                                                                  ataACAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAg    <  1:400441/140‑1 (MQ=255)                                                                                                                                    aaCAGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAgcg  <  2:1047591/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         TGCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCG  >  NC_000913/3680986‑3681256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑