Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,326,978	Δ1 bp	intergenic (‑244/‑157)	yjdM ← / → crfC	conserved protein YjdM/clamp‑binding sister replication fork colocalization protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,326,978	0	A	.	100.0%	297.6 / NA	69	intergenic (‑244/‑157)	yjdM/crfC	conserved protein YjdM/clamp‑binding sister replication fork colocalization protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (39/30);  total (39/30)

TTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTGACGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACTCAAAAACAATCTGGCTAAATAAAATAACAAA  >  NC_000913/4326844‑4327110                                                                                                                                       |                                                                                                                                     tttAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGtttt                                                                                                                                <  2:1913712/140‑1 (MQ=255)    aGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTaaa                                                                                                                             <  2:1386685/140‑1 (MQ=255)    aGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTaaa                                                                                                                             <  1:1709515/140‑1 (MQ=255)      acgacgATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTa                                                                                                                               <  1:1925690/136‑1 (MQ=255)      acgacgATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTa                                                                                                                               >  2:1925690/1‑136 (MQ=255)           gaTCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACg                                                                                                                           >  2:1158830/1‑135 (MQ=255)           gaTCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACg                                                                                                                           <  1:1158830/135‑1 (MQ=255)           gaTCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCa                                                                                                                      >  1:258362/1‑140 (MQ=255)             tCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCaga                                                                                                                    <  2:126812/140‑1 (MQ=255)             tCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCaga                                                                                                                    <  2:1495657/140‑1 (MQ=255)               gTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCagaag                                                                                                                  <  2:1488726/140‑1 (MQ=255)                ttttCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCagaaga                                                                                                                 <  1:1373954/140‑1 (MQ=255)                ttttCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCagaaga                                                                                                                 >  1:1042511/1‑140 (MQ=255)                  ttCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACg                                                                                                               <  2:1333383/140‑1 (MQ=255)                                        aaGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAaaca                                                                                         >  2:1898880/1‑140 (MQ=255)                                         aGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAaacaa                                                                                        >  2:398161/1‑140 (MQ=255)                                         aGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAaacaa                                                                                        >  1:1873813/1‑140 (MQ=255)                                               taatCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACt                                                                                  <  1:1363189/140‑1 (MQ=255)                                               taatCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACt                                                                                  <  2:1594288/140‑1 (MQ=255)                                               taatCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACt                                                                                  <  2:1304761/140‑1 (MQ=255)                                                   cTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAat                                                                              <  2:1291875/140‑1 (MQ=255)                                                    tttttGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACt                                                                                  >  2:1288282/1‑135 (MQ=255)                                                    tttttGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACt                                                                                  <  1:1288282/135‑1 (MQ=255)                                                    tttttGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAata                                                                             <  2:1250963/140‑1 (MQ=255)                                                     ttttGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAatat                                                                            >  2:1331465/1‑140 (MQ=255)                                                                    ttattTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGc                                                             >  1:1054399/1‑140 (MQ=255)                                                                     tattTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCa                                                            >  2:1701717/1‑140 (MQ=255)                                                                     tattTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCa                                                            <  1:1878839/140‑1 (MQ=255)                                                                     tattTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCa                                                            <  2:258362/140‑1 (MQ=255)                                                                          aaGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAAt                                                       >  1:1613031/1‑140 (MQ=255)                                                                          aaGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAAt                                                       >  2:1533175/1‑140 (MQ=255)                                                                          aaGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAAt                                                       >  1:579170/1‑140 (MQ=255)                                                                          aaGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAAt                                                       >  1:487112/1‑140 (MQ=255)                                                                               aaTGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATaa                                                  >  2:1003988/1‑140 (MQ=255)                                                                                 tGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATaaaa                                                >  2:870912/1‑140 (MQ=255)                                                                                 tGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATaaaa                                                >  1:930054/1‑140 (MQ=255)                                                                                 tGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATaaaa                                                >  2:81693/1‑140 (MQ=255)                                                                                 tGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATaaaa                                                >  2:569074/1‑140 (MQ=255)                                                                                 tGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATaaaa                                                <  1:569074/140‑1 (MQ=255)                                                                                    tAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGcc                                             <  2:944154/140‑1 (MQ=255)                                                                                    tAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGcc                                             <  2:487112/140‑1 (MQ=255)                                                                                     aaTGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCg                                            >  1:764624/1‑140 (MQ=255)                                                                                      aTGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGa                                           >  1:287339/1‑140 (MQ=255)                                                                                      aTGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGa                                           >  2:1696990/1‑140 (MQ=255)                                                                                       tGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGaa                                          >  2:1248675/1‑140 (MQ=255)                                                                                         ctctACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGaaaa                                        >  2:1681882/1‑140 (MQ=255)                                                                                           ctACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAACAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACa                                      >  2:1424862/1‑140 (MQ=255)                                                                                           ctACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACa                                      >  1:338656/1‑140 (MQ=255)                                                                                           ctACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACa                                      >  2:1861465/1‑140 (MQ=255)                                                                                            tACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAg                                     <  1:1331465/140‑1 (MQ=255)                                                                                            tACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAg                                     >  2:1501847/1‑140 (MQ=255)                                                                                                tGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACt                                 <  1:1248675/140‑1 (MQ=255)                                                                                                  ttCTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACTCa                               >  2:62934/1‑140 (MQ=255)                                                                                                    cTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACTCaaa                             >  1:653752/1‑140 (MQ=255)                                                                                                    cTTTTTTTAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACTCaaa                             <  1:870912/140‑1 (MQ=255)                                                                                                      ttttttAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACTCaaaaa                           >  1:1677829/1‑140 (MQ=255)                                                                                                      ttttttAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACTCaaaaa                           >  1:1504792/1‑140 (MQ=255)                                                                                                        ttttAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACTCAAAAACa                         >  1:1856641/1‑140 (MQ=255)                                                                                                         tttAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACTCAAAAACaa                        <  1:557522/140‑1 (MQ=255)                                                                                                          ttAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACTCAAAAACAAt                       <  1:1898880/140‑1 (MQ=255)                                                                                                          ttAATTCACATGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACTCAAAAACAAt                       <  2:1504792/140‑1 (MQ=255)                                                                                                                 acaTGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACt                                 <  2:250861/123‑1 (MQ=255)                                                                                                                 acaTGATATTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACt                                 >  1:250861/1‑123 (MQ=255)                                                                                                                        atTTTTATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACTCAAAAACAATCTGGCTaaataaaa         >  2:1727000/1‑140 (MQ=255)                                                                                                                         tttttATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACTCAAAAACAATCTGGCTaaataaaat        <  1:1701717/140‑1 (MQ=255)                                                                                                                          ttttATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACTCAAAAACAATCTGGCTaaataaaata       <  2:1042511/140‑1 (MQ=255)                                                                                                                             tATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACTCAAAAACAATCTGGCTAAATAAAATAACa    >  2:1081536/1‑140 (MQ=255)                                                                                                                             tATCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACTCAAAAACAATCTGGCTAAATAAAATAACa    >  2:605077/1‑140 (MQ=255)                                                                                                                               tCTTTCTG‑CGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACTCAAAAACAATCTGGCTAAATAAAATAACaaa  >  1:1461552/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     TTTAGACGACGATCGTTTTCATTTGAAAGGAAACGCAAAAAGAAAATAATCTTTTTGCCTCTATTTATTATTTAAGATAATGTTAATGCTCTACTTGTTCTTTTTTTAATTCACATGATATTTTTATCTTTCTGACGTTTTAAACGTATCAGAAGACGTTATTTATTCTGATAAAAAACAACACACTAAATATAAAATTATTTCTTGCAGTAATTATAAAAGCCGAAAACAGAACTCAAAAACAATCTGGCTAAATAAAATAACAAA  >  NC_000913/4326844‑4327110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑