Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,093,253	A→G	I286V (ATC→GTC)	hisC →	histidinol‑phosphate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,093,253	0	A	G	100.0%	309.0 / NA	82	I286V (ATC→GTC)	hisC	histidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/46);  total (36/46)

TCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGATCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGATCAGAATAAACAACCC  >  NC_000913/2093116‑2093381                                                                                                                                          |                                                                                                                                 tCGCCCCCTACCCGCTCTCGACGCCGGTTGCTGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTc                                                                                                                                >  1:27113/1‑140 (MQ=255) tCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTc                                                                                                                                >  2:1071623/1‑140 (MQ=255) tCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTc                                                                                                                                >  2:1413799/1‑140 (MQ=255)   gCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTccc                                                                                                                              <  2:1054404/140‑1 (MQ=255)   gCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTccc                                                                                                                              <  1:1126672/140‑1 (MQ=255)   gCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTccc                                                                                                                              <  1:1212658/140‑1 (MQ=255)   gCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTccc                                                                                                                              <  1:757978/140‑1 (MQ=255)   gCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTccc                                                                                                                              <  2:1925887/140‑1 (MQ=255)      cccTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTg                                                                                                                           <  2:1385439/140‑1 (MQ=255)      cccTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTg                                                                                                                           <  1:1439425/140‑1 (MQ=255)        cTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCg                                                                                                                         >  1:711535/1‑140 (MQ=255)          aCCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTa                                                                                                                       <  2:1173160/140‑1 (MQ=255)               ctctCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCa                                                                                                                  >  2:1592655/1‑140 (MQ=255)               ctctCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCa                                                                                                                  >  1:658507/1‑140 (MQ=255)               ctctCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCa                                                                                                                  >  1:163392/1‑140 (MQ=255)               ctctCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCa                                                                                                                  >  1:1398677/1‑140 (MQ=255)               ctctCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCa                                                                                                                  >  1:263867/1‑140 (MQ=255)               ctctCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCa                                                                                                                  >  1:171662/1‑140 (MQ=255)                   cGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGtt                                                                                                              >  2:619186/1‑140 (MQ=255)                   cGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGAATGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGtt                                                                                                              >  2:470221/1‑140 (MQ=255)                     aCGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGtttt                                                                                                            <  1:1724703/140‑1 (MQ=255)                          ggTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGAct                                                                                                       <  1:626809/140‑1 (MQ=255)                             tGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTg                                                                                                    <  1:1413799/140‑1 (MQ=255)                             tGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTg                                                                                                    <  1:1112337/140‑1 (MQ=255)                              gCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGa                                                                                                   <  1:1671849/140‑1 (MQ=255)                               ccGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGaa                                                                                                  <  1:619186/140‑1 (MQ=255)                                 gACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAAc                                                                                                <  1:1318082/140‑1 (MQ=255)                                  aCATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAAcc                                                                                               <  2:1310577/140‑1 (MQ=255)                                  aCATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTGCGGCTCTGAAAcc                                                                                               <  2:1398677/140‑1 (MQ=255)                                    aTTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGAATGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCaa                                                                                             <  1:470221/140‑1 (MQ=255)                                           cccAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACAtt                                                                                      <  1:1421374/140‑1 (MQ=255)                                                gCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGgc                                                                                 <  2:1052612/140‑1 (MQ=255)                                                gCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGgc                                                                                 >  2:531906/1‑140 (MQ=255)                                                  gTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGgcgc                                                                               <  2:263867/140‑1 (MQ=255)                                                          cacaGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCttt                                                                          >  2:1666252/1‑137 (MQ=255)                                                          cacaGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCttt                                                                          <  1:1666252/137‑1 (MQ=255)                                                          cacaGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTaaa                                                                       >  1:1562992/1‑140 (MQ=255)                                                          cacaGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTaaa                                                                       >  2:1412918/1‑140 (MQ=255)                                                           acaGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCttt                                                                          <  1:598145/136‑1 (MQ=255)                                                           acaGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCttt                                                                          >  2:598145/1‑136 (MQ=255)                                                           acaGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAg                                                                      >  1:1556434/1‑140 (MQ=255)                                                             aGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGcc                                                                    >  1:342702/1‑140 (MQ=255)                                                                  aTCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAg                                                               >  1:1681559/1‑140 (MQ=255)                                                                      tcgCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCg                                                           <  1:276227/140‑1 (MQ=255)                                                                      tcgCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCg                                                           <  1:1749584/140‑1 (MQ=255)                                                                      tcgCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCg                                                           >  1:1736933/1‑140 (MQ=255)                                                                      tcgCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCg                                                           <  2:163392/140‑1 (MQ=255)                                                                         cnATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGgtn                                                        >  1:1439896/1‑139 (MQ=255)                                                                         ccATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGgtg                                                        >  1:830881/1‑140 (MQ=255)                                                                                     gggTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTg                                                             <  1:1343311/123‑1 (MQ=255)                                                                                     gggTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTg                                                             >  2:1343311/1‑123 (MQ=255)                                                                                      ggTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTtgt                                           <  2:1562992/140‑1 (MQ=255)                                                                                            gcAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATc                                     <  2:1556434/140‑1 (MQ=255)                                                                                             cAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCa                                    <  1:1071623/140‑1 (MQ=255)                                                                                                attattGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAggg                                 >  1:953826/1‑140 (MQ=255)                                                                                                attattGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAggg                                 >  1:58828/1‑140 (MQ=255)                                                                                                 ttattGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGc                                <  2:171662/140‑1 (MQ=255)                                                                                                 ttattGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGc                                <  2:342702/140‑1 (MQ=255)                                                                                                  tattGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGTGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGTCa                               <  1:1592655/140‑1 (MQ=255)                                                                                                  tattGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCa                               <  2:1681559/140‑1 (MQ=255)                                                                                                     tGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGc                                <  1:627804/136‑1 (MQ=255)                                                                                                     tGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGc                                >  2:627804/1‑136 (MQ=255)                                                                                                     tGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTa                            >  1:476230/1‑140 (MQ=255)                                                                                                          aaCGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTa                       >  1:408644/1‑140 (MQ=255)                                                                                                           aCGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTAc                      <  1:1047047/140‑1 (MQ=255)                                                                                                           aCGCGAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTAc                      <  2:27113/140‑1 (MQ=255)                                                                                                               gAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGa                  <  2:953826/140‑1 (MQ=255)                                                                                                               gAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGa                  <  2:832840/140‑1 (MQ=255)                                                                                                               gAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGa                  <  2:658507/140‑1 (MQ=255)                                                                                                               gAATACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGa                  <  2:1736933/140‑1 (MQ=255)                                                                                                                aaTACCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGAt                 <  2:476230/140‑1 (MQ=255)                                                                                                                   aCCTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGATCAg              >  1:1216360/1‑140 (MQ=255)                                                                                                                    ccTGATTGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGATCAGa             >  2:1782359/1‑140 (MQ=255)                                                                                                                         ttGCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGATCAGAATaaa        >  1:923196/1‑140 (MQ=255)                                                                                                                           gCCGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGATCAGAATAaaca      >  2:1813455/1‑140 (MQ=255)                                                                                                                             cGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGATCAGAATAaacaac    <  1:531906/140‑1 (MQ=255)                                                                                                                             cGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGATCAGAATAaacaa     >  1:221645/1‑139 (MQ=255)                                                                                                                             cGCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGATCAGAATAaacaa     <  2:221645/139‑1 (MQ=255)                                                                                                                              gCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGATCAGAATAAACAAcc   <  1:1813455/140‑1 (MQ=255)                                                                                                                              gCACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGATCAGAATAAACAAcc   <  2:1216360/140‑1 (MQ=255)                                                                                                                               cACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGATCAGAATAAACAAccc  >  2:541044/1‑140 (MQ=255)                                                                                                                               cACTGAAAGAGGTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGATCAGAATAAACAAccc  >  1:533598/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                 TCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCGAATACCTGATTGCCGCACTGAAAGAGATCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGATCAGAATAAACAACCC  >  NC_000913/2093116‑2093381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑