Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	33,779	C→A	P988Q (CCG→CAG)	carB →	carbamoyl phosphate synthetase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	33,779	0	C	A	100.0%	284.8 / NA	80	P988Q (CCG→CAG)	carB	carbamoyl phosphate synthetase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/49);  total (31/49)

GGTCGTGCGCTGCTTTCCGTGCGCGAAGGCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCG  >  NC_000913/33643‑33915                                                                                                                                         |                                                                                                                                         ggTCGTGCGCTGCTTTCCGTGCGCGAAGGCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCagcg                                                                                                                                       >  2:139847/1‑140 (MQ=255)  gTCGTGCGCTGCTTTCCGTGCGCGAAGGCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGt                                                                                                                                      >  1:1175794/1‑140 (MQ=255)     gTGCGCTGCTTTCCGTGCGCGAAGGCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTg                                                                                                                                   >  2:477510/1‑140 (MQ=255)      tGCGCTGCTTTCCGTGCGCGAAGGCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTgg                                                                                                                                  >  1:143737/1‑140 (MQ=255)      tGCGCTGCTTTCCGTGCGCGAAGGCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTgg                                                                                                                                  >  2:617571/1‑140 (MQ=255)            gctTTCCGTGCGCGAAGGCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACa                                                                                                                            <  1:575608/140‑1 (MQ=255)            gctTTCCGTGCGCGAAGGCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACa                                                                                                                            <  1:248398/140‑1 (MQ=255)             ctTTCCGTGCGCGAAGGCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACaa                                                                                                                           >  1:1090161/1‑140 (MQ=255)               ttCCGTGCGCGAAGGCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAgg                                                                                                                         <  1:97405/140‑1 (MQ=255)               ttCCGTGCGCGAAGGCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAgg                                                                                                                         <  2:731788/140‑1 (MQ=255)                  cGTGCGCGAAGGCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGc                                                                                                                      >  2:247602/1‑140 (MQ=255)                       gcgAAGGCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAACGTCTGGTAAACAAGGTGCATGaa                                                                                                                 >  1:209416/1‑140 (MQ=255)                       gcgAAGGCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATCGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGaa                                                                                                                 >  2:1212066/1‑140 (MQ=255)                          aaGGCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGc                                                                                                              <  2:486658/140‑1 (MQ=255)                          aaGGCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGc                                                                                                              <  2:1175794/140‑1 (MQ=255)                            ggCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCg                                                                                                            <  1:833540/140‑1 (MQ=255)                            ggCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCg                                                                                                            <  1:498325/140‑1 (MQ=255)                            ggCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCg                                                                                                            <  1:312879/140‑1 (MQ=255)                              cGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTc                                                                                                          <  2:1073487/140‑1 (MQ=255)                                 tAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGc                                                                                                       <  2:1004948/140‑1 (MQ=255)                                 tAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGc                                                                                                       <  1:193855/140‑1 (MQ=255)                                      aaCGCGTGGTGGGCCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACAtt                                                                                                  <  2:246928/140‑1 (MQ=255)                                       aCGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTc                                                                                                 >  2:133944/1‑140 (MQ=255)                                       aCGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTc                                                                                                 >  2:538334/1‑140 (MQ=255)                                       aCGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGCGCATGAAGGCCGTCCGCACATTc                                                                                                 >  2:650693/1‑140 (MQ=255)                                          cgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAgg                                                                                              <  1:247602/140‑1 (MQ=255)                                           gtggtgGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGa                                                                                             <  1:1032315/140‑1 (MQ=255)                                            tggtggACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGAc                                                                                            <  1:68980/140‑1 (MQ=255)                                            tggtggACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGAc                                                                                            <  1:802834/140‑1 (MQ=255)                                            tggtggACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGAc                                                                                            <  1:133944/140‑1 (MQ=255)                                             ggtggACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGAcc                                                                                           >  1:1199557/1‑140 (MQ=255)                                             ggtggACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATCGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGAcc                                                                                           <  1:1212066/140‑1 (MQ=255)                                             agtggACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGAcc                                                                                           <  1:438778/139‑1 (MQ=255)                                              gtggACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGa                                                                                             <  1:76503/137‑1 (MQ=255)                                              gtggACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGa                                                                                             >  2:76503/1‑137 (MQ=255)                                              gtggACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCg                                                                                          >  2:563258/1‑140 (MQ=255)                                                ggACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGc                                                                                                       >  1:902188/1‑125 (MQ=255)                                                ggACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGc                                                                                                       <  2:902188/125‑1 (MQ=255)                                                ggACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACAtt                                                                                                  >  1:1111573/1‑130 (MQ=255)                                                ggACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACAtt                                                                                                  <  2:1111573/130‑1 (MQ=255)                                                ggACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTa                                                                                        >  1:104736/1‑140 (MQ=255)                                                      ggcggcAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGa                                                                                  <  1:650693/140‑1 (MQ=255)                                                       gcggcAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGaa                                                                                 >  2:760220/1‑140 (MQ=255)                                                        cggcAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAAt                                                                                >  2:496368/1‑140 (MQ=255)                                                         ggcAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATg                                                                               <  2:1199557/140‑1 (MQ=255)                                                          gcAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATgg                                                                              <  1:1239924/140‑1 (MQ=255)                                                           cAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGc                                                                             <  1:477510/140‑1 (MQ=255)                                                           cAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGc                                                                             >  2:1010927/1‑140 (MQ=255)                                                           cAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGc                                                                             <  1:181356/140‑1 (MQ=255)                                                             aaaCTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGa                                                                           <  1:418782/140‑1 (MQ=255)                                                             aaaCTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGa                                                                           <  2:671356/140‑1 (MQ=255)                                                              aaCTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGaa                                                                          <  2:1084875/140‑1 (MQ=255)                                                              aaCTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGaa                                                                          <  1:538334/140‑1 (MQ=255)                                                              aaCTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGaa                                                                          <  2:191739/140‑1 (MQ=255)                                                               aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAat                                                                         <  1:2856/140‑1 (MQ=255)                                                                 tgctgAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCg                                                                            >  1:122373/1‑135 (MQ=255)                                                                 tgctgAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCg                                                                            <  2:122373/135‑1 (MQ=255)                                                                   ctgAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATAc                                                                     <  1:550197/140‑1 (MQ=255)                                                                   ctgAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATAc                                                                     <  1:139847/140‑1 (MQ=255)                                                                      aaaCAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTa                                                                  <  1:496368/140‑1 (MQ=255)                                                                        aCAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTAca                                                                <  2:1090161/140‑1 (MQ=255)                                                                        aCAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTAca                                                                <  1:1105042/140‑1 (MQ=255)                                                                           gggCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTAcatca                                                             >  2:861679/1‑140 (MQ=255)                                                                           gggCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTAcatca                                                             >  1:240811/1‑140 (MQ=255)                                                                             gCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACatcatc                                                           >  2:335031/1‑140 (MQ=255)                                                                                  gAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAacac                                                      <  1:14661/140‑1 (MQ=255)                                                                                      tGGCTGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCCGGAATCAACCACCGTCTGGTAAACAAGGAGCATGAAGGCCGTCCGCACATTCAGGACCGCATCAAGAATGGCGAATATACCTACATCATCAACaccacc                                                  >  2:1015286/1‑140 (MQ=255)                                                                                        gATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTc                                                >  2:298234/1‑140 (MQ=255)                                                                                                  acggcacggcGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCcgtcgt                                      >  1:515352/1‑140 (MQ=255)                                                                                                   cggcacggcGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTg                                     <  2:143737/140‑1 (MQ=255)                                                                                                   cggcacggcGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTg                                     <  1:760220/140‑1 (MQ=255)                                                                                                        cggcGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTGCATCATCAACACCACCTCAGGCCGTCGTGCGAtt                                >  1:1087721/1‑140 (MQ=255)                                                                                                           cGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGaa                             <  1:1010927/140‑1 (MQ=255)                                                                                                           cGATTGTGCTGGGCGAAGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGaa                             <  1:617571/140‑1 (MQ=255)                                                                                                                           aGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATtc             >  1:417014/1‑140 (MQ=255)                                                                                                                           aGCAGGTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGCCCGTCGTGCGATTGAAGACTCCCGCGTGATtc             >  1:1017339/1‑140 (MQ=255)                                                                                                                                gTATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGc        >  2:52907/1‑140 (MQ=255)                                                                                                                                 tATCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCa       <  2:240811/140‑1 (MQ=255)                                                                                                                                  aTCAACCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAg      >  1:226452/1‑140 (MQ=255)                                                                                                                                      aCCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTgcg  <  2:417014/140‑1 (MQ=255)                                                                                                                                      aCCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTgcg  <  1:166093/140‑1 (MQ=255)                                                                                                                                      aCCAGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTgcg  <  2:1017339/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GGTCGTGCGCTGCTTTCCGTGCGCGAAGGCGATAAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCG  >  NC_000913/33643‑33915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑