Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,196,404	A→T	I117N (ATC→AAC)	rfaE ←	fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,196,404	0	A	T	100.0%	242.4 / NA	70	I117N (ATC→AAC)	rfaE	fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (41/29);  total (41/29)

TGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGATCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAGCGCGCGCGCTGCATCGTCAATGCCCGTCA  >  NC_000913/3196272‑3196539                                                                                                                                     |                                                                                                                                        tgTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTg                                                                                                                                  >  1:448564/1‑140 (MQ=255) tgTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTg                                                                                                                                  >  1:224189/1‑140 (MQ=255)  gTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTgt                                                                                                                                 >  1:218787/1‑140 (MQ=255)   tACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTgtt                                                                                                                                >  1:816622/1‑140 (MQ=255)   tACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTgtt                                                                                                                                >  1:370732/1‑140 (MQ=255)     cGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTgttgg                                                                                                                              >  2:349721/1‑140 (MQ=255)      gCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTgttggt                                                                                                                             <  1:108657/140‑1 (MQ=255)              gcgcACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGaa                                                                                                                     <  2:224189/140‑1 (MQ=255)              gcgcACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGaa                                                                                                                     <  1:1195384/140‑1 (MQ=255)                   ccTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTAc                                                                                                                <  1:752572/140‑1 (MQ=255)                    cTTTGGCGTAGTCAGAAAGCACCTGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTAcc                                                                                                               >  1:1094623/1‑140 (MQ=255)                    cTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTAcc                                                                                                               <  1:1069231/140‑1 (MQ=255)                    cTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTAcc                                                                                                               >  2:519723/1‑140 (MQ=255)                    cTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTAcc                                                                                                               >  1:1192402/1‑140 (MQ=255)                      ttGGCGTAGTCAGAAAGCACCTGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCg                                                                                                             <  2:1094623/140‑1 (MQ=255)                      ttGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCg                                                                                                             <  1:703954/140‑1 (MQ=255)                        ggCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCg                                                                                                             >  2:658893/1‑138 (MQ=255)                        ggCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCg                                                                                                             <  1:658893/138‑1 (MQ=255)                          cGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAAt                                                                                                         <  1:349721/140‑1 (MQ=255)                            tAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCATAAttt                                                                                                       <  2:370732/140‑1 (MQ=255)                              gTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTgg                                                                                                     >  2:924273/1‑140 (MQ=255)                              gTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTgg                                                                                                     <  1:992544/140‑1 (MQ=255)                              gTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTgg                                                                                                     >  1:373647/1‑140 (MQ=255)                                 aGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTaa                                                                                                  >  2:635424/1‑140 (MQ=255)                                   aaaGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATg                                                                                                >  1:897299/1‑140 (MQ=255)                                        aCCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCgg                                                                                           >  2:371334/1‑140 (MQ=255)                                        aCCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCgg                                                                                           >  1:700366/1‑140 (MQ=255)                                        aCCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGCACCCGTAATTTGGTAATGGTCgg                                                                                           >  1:485020/1‑140 (MQ=255)                                         ccAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGa                                                                                          <  2:317227/140‑1 (MQ=255)                                             cgcgcCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCg                                                                                      >  1:1028702/1‑140 (MQ=255)                                              gcgcCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGTAAAGTACCCGTAATTTGGTAATGGTCGGATGCGt                                                                                     <  1:1076644/140‑1 (MQ=255)                                                   aaTCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTa                                                                                <  2:700366/140‑1 (MQ=255)                                                        aaCTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGaa                                                                           >  2:1193000/1‑140 (MQ=255)                                                          cTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAAc                                                                         >  2:941251/1‑140 (MQ=255)                                                             aGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGaa                                                                      >  1:908238/1‑140 (MQ=255)                                                              gcgcCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAg                                                                     >  2:116053/1‑140 (MQ=255)                                                               cgcCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGt                                                                                     <  2:557281/123‑1 (MQ=255)                                                               cgcCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGt                                                                                     >  1:557281/1‑123 (MQ=255)                                                               cgcCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGt                                                                    <  2:373647/140‑1 (MQ=255)                                                               cgcCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGt                                                                    <  2:93903/140‑1 (MQ=255)                                                                  cTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGc                                                                 >  2:1103804/1‑140 (MQ=255)                                                                     aTTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCAtt                                                              <  1:1193000/140‑1 (MQ=255)                                                                     aTTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCAtt                                                              >  2:62076/1‑140 (MQ=255)                                                                     aTTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGCACAGAAACGAAGTCGCAtt                                                              >  1:174172/1‑140 (MQ=255)                                                                               tCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATttgacgttgac                                                    >  1:985230/1‑140 (MQ=255)                                                                                    cAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCgg                                               >  1:1144774/1‑140 (MQ=255)                                                                                       cGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCa                                            <  2:897299/140‑1 (MQ=255)                                                                                       cGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCa                                            <  2:816622/140‑1 (MQ=255)                                                                                       cGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCa                                            <  2:985230/140‑1 (MQ=255)                                                                                       cGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCa                                            <  1:62076/140‑1 (MQ=255)                                                                                         gCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCaga                                          >  2:230185/1‑140 (MQ=255)                                                                                             cGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGAtt                                      >  2:733534/1‑140 (MQ=255)                                                                                                       ccTTCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAgcgc                            <  2:1028702/140‑1 (MQ=255)                                                                                                          tCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAgcgcgcg                         >  1:206087/1‑140 (MQ=255)                                                                                                          tCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAgcgcgcg                         >  1:327165/1‑140 (MQ=255)                                                                                                          tCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAgcgcgcg                         >  1:435090/1‑140 (MQ=255)                                                                                                          tCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAgcgcgcg                         >  1:522861/1‑140 (MQ=255)                                                                                                          tCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAgcgcgcg                         >  2:1214095/1‑140 (MQ=255)                                                                                                          tCGAAACCTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAgcgcgcg                         <  2:368752/140‑1 (MQ=255)                                                                                                                ccTTCTTCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAGCGCGCGCGCTGc                   >  1:428652/1‑140 (MQ=255)                                                                                                                 cttcttCAAAATCGAGACGGTTCAGCTGGTGGTTGCGGGAAAGTAACCGTAATTTGGTAATGGTCAGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACACAGCGCGCGCGCTGCa                  <  2:485020/140‑1 (MQ=255)                                                                                                                 cttcttCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAGCGCGCGCGCTGCa                  <  2:206087/140‑1 (MQ=255)                                                                                                                 cttcttCAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAGCGCGCGCGCTGCa                  <  1:173373/140‑1 (MQ=255)                                                                                                                  ttcttcAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAGCGCGCGCGCTGCAt                 >  2:1133060/1‑140 (MQ=255)                                                                                                                  ttcttcAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAGCGCGCGCGCTGCAt                 >  1:534441/1‑140 (MQ=255)                                                                                                                    cttcAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAGCGCGCGCGCTGCATCg               >  1:1116009/1‑140 (MQ=255)                                                                                                                     ttcAAAATCCAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGTGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAGCGCGCGCGCTGCATCGt              <  1:371334/140‑1 (MQ=255)                                                                                                                             ccAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAGCGCGCGCGCTGCATCGTCAATGccc      <  1:635424/140‑1 (MQ=255)                                                                                                                              cAGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAGCGCGCGCGCTGCATCGTCAATGCCCg     <  1:116053/140‑1 (MQ=255)                                                                                                                               aGACGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGT‑CAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAGCGCGCGCGCTGCATCGTCAATGCCCGTc   >  2:284989/1‑140 (MQ=255)                                                                                                                                 aCGGTTCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAGCGCGCGCGCTGCATCGTCAATGCCCGTCa  >  1:125092/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        TGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAGCGGCTGCGGATCAACACCTTCGAAACCTTCTTCAAAATCCAGACGGATCAGCTGTTGGTTGCGGGAAAGTACCCGTAATTTGGTAATGGTCGGATGCGTCGGTACAGAAACGAAGTCGCATTTGACGTTGACGTCGGCCAGAGATTTACTCAGCGCGCGCGCTGCATCGTCAATGCCCGTCA  >  NC_000913/3196272‑3196539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑