Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,883	+C	coding (667/687 nt)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,879	1	.	C	100.0%	213.5 / NA	57	E224G (GAA→GGA)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (25/32);  total (25/32)

ATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATT‑CCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC  >  NC_000913/3815743‑3816012                                                                                                                                            |                                                                                                                                        aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATT‑ccc                                                                                                                                      >  2:1668000/1‑140 (MQ=255)    aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATT‑ccccct                                                                                                                                   <  2:1358327/140‑3 (MQ=255)      cTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATT‑ccccctcg                                                                                                                                 >  1:756209/1‑136 (MQ=255)        ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCggg                                                                                                                                >  2:1553282/1‑140 (MQ=255)         gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGc                                                                                                                               <  2:756209/140‑1 (MQ=255)         gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGc                                                                                                                               <  1:1457231/140‑1 (MQ=255)          cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGcc                                                                                                                              >  1:1680895/1‑140 (MQ=255)             tGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCaga                                                                                                                           <  2:867620/140‑1 (MQ=255)              gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCagag                                                                                                                          <  2:1234359/140‑1 (MQ=255)                tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGcc                                                                                                                        <  1:593075/140‑1 (MQ=255)                 atGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCca                                                                                                                       >  2:1366565/1‑140 (MQ=255)                   ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCcaac                                                                                                                     <  2:210126/140‑1 (MQ=255)                      ttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAg                                                                                                                  <  1:1688707/140‑1 (MQ=255)                      ttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAg                                                                                                                  <  1:1398695/140‑1 (MQ=255)                         aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑g                                                                                                             >  2:566039/1‑140 (MQ=255)                          tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑Ga                                                                                                            >  2:43888/1‑140 (MQ=255)                          tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAAGCGACTTCTTACTTCGCTTTAAAATTCGCTTTCCCACGGCCGCCTCCTGCGTCGC‑ACAAGGGGTTCCGATT‑CCCCCCGGGCCGGAACCAAAAAggttg                                                                                                              >  2:1522864/1‑136 (MQ=255)                              ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAg                                                                                                        <  1:923201/140‑1 (MQ=255)                              ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAg                                                                                                        <  2:1680895/140‑1 (MQ=255)                               tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGc                                                                                                       >  1:879650/1‑140 (MQ=255)                                cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGct                                                                                                      >  1:149072/1‑140 (MQ=255)                                     ctcATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCa                                                                                                 <  2:110010/140‑1 (MQ=255)                                     ctcATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCa                                                                                                 >  2:796393/1‑140 (MQ=255)                                     ctcATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCa                                                                                                 <  1:517628/140‑1 (MQ=255)                                     ctcATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCa                                                                                                 <  2:1296436/140‑1 (MQ=255)                                           ttACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTg                                                                                           <  1:796393/140‑1 (MQ=255)                                             aCTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGaa                                                                                         >  1:1567337/1‑140 (MQ=255)                                                 ttCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGc                                                                                     >  2:134278/1‑140 (MQ=255)                                                    tACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATAGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCg                                                                                  <  2:1556134/140‑1 (MQ=255)                                                                  aGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTccc                                                                    <  2:1614647/140‑1 (MQ=255)                                                                     cGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  2:1151286/140‑1 (MQ=255)                                                                     cGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  1:48653/140‑1 (MQ=255)                                                                        c‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCAc                                                              >  1:861273/1‑140 (MQ=255)                                                                                   gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg                                                    >  1:551005/1‑140 (MQ=255)                                                                                   gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg                                                    >  1:613153/1‑140 (MQ=255)                                                                                      ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc                                                 <  1:662077/140‑1 (MQ=255)                                                                                       tAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGcc                                                <  1:1522864/140‑1 (MQ=255)                                                                                       tAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGcc                                                <  1:43888/140‑1 (MQ=255)                                                                                              cAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTc                                         <  2:1567337/140‑1 (MQ=255)                                                                                               aGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCg                                        >  2:450485/1‑140 (MQ=255)                                                                                               aGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCg                                        >  2:457475/1‑140 (MQ=255)                                                                                               aGTTTGCCAGCGCCGCCTCCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCg                                        >  2:547687/1‑140 (MQ=255)                                                                                                gTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCgg                                       >  2:928314/1‑140 (MQ=255)                                                                                                 tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGTTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                      >  2:1548367/1‑140 (MQ=255)                                                                                                 tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                      <  1:1065611/140‑1 (MQ=255)                                                                                                 tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                      <  1:1553282/140‑1 (MQ=255)                                                                                                   tGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtca                                    <  2:571032/140‑1 (MQ=255)                                                                                                      cAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcatca                                 <  1:889429/140‑1 (MQ=255)                                                                                                       aGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCAc                                >  1:673034/1‑140 (MQ=255)                                                                                                        gcgcCGCCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACt                               <  2:861273/140‑1 (MQ=255)                                                                                                         cgccgcCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCt                                           >  2:1338706/1‑127 (MQ=255)                                                                                                         cgccgcCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCt                                           <  1:1338706/127‑1 (MQ=255)                                                                                                         cgccgcCTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATTACTa                              >  1:1403131/1‑140 (MQ=255)                                                                                                           ccgccTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACg                            >  1:802779/1‑140 (MQ=255)                                                                                                            cgccTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGt                           <  1:374854/140‑1 (MQ=255)                                                                                                              ccTTCTGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTc                         >  1:993826/1‑140 (MQ=255)                                                                                                                   tGCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTc                    >  2:215026/1‑140 (MQ=255)                                                                                                                    gCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAGCGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCg                   <  1:450485/140‑1 (MQ=255)                                                                                                                    gCGTCGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCg                   <  1:1668000/140‑1 (MQ=255)                                                                                                                        cGCTACAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGtct               >  1:592847/1‑140 (MQ=255)                                                                                                                             cAATGGATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCg          >  1:1074595/1‑140 (MQ=255)                                                                                                                                 ggATT‑CGATTCCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCag      <  1:215026/140‑1 (MQ=255)                                                                                                                                  gatt‑cgattcCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCaga     <  1:1366565/140‑1 (MQ=255)                                                                                                                                  gatt‑cgattcCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCaga     <  2:48563/140‑1 (MQ=255)                                                                                                                                    tt‑cgattcCCCCTCGGGCCAGAGCCAACAAGATGAGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGCCGGTCTCTGCGGCaga     >  2:626783/1‑140 (MQ=255)                                                                                                                                    tt‑cgattcCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCGCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGt   >  1:1256587/1‑140 (MQ=255)                                                                                                                                    tt‑cgattcCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGt   >  2:1459507/1‑140 (MQ=255)                                                                                                                                     t‑cgattcCCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTc  >  1:49055/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                        ATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGAC‑TCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATT‑CGATT‑CCCCTCGGGCCAGAGCCAACAAGAT‑‑GAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC  >  NC_000913/3815743‑3816012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑