Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,326,4500TG23.8% 171.9 / 46.1 84G114G (GGT→GGG) atoBacetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (4/60);  new base G (20/0);  total (24/60)
Fisher's exact test for biased strand distribution p-value = 9.90e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 102 total aligned reads displayed.

GTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTA  >  NC_000913/2326318‑2326589
                                                                                                                                    |                                                                                                                                            
 ttAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGaaaa                                                                                                                                      >  2:68050/1‑140 (MQ=255)
   aaaaaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAat‑a                                                                                                                                   <  2:213261/140‑1 (MQ=255)
     aaaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAat‑att                                                                                                                                 >  1:215636/1‑139 (MQ=255)
     aaaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAT‑ATg                                                                                                                                 >  2:952481/1‑140 (MQ=255)
         cGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGtt                                                                                                                             <  1:837974/140‑1 (MQ=255)
         cGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGtt                                                                                                                             <  2:1458685/140‑1 (MQ=255)
         cGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGtt                                                                                                                             <  1:1527453/140‑1 (MQ=255)
         cGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGtt                                                                                                                             >  2:1374997/1‑140 (MQ=255)
         cGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGTTTGAAAATAATGAGt                                                                                                                              >  2:304903/1‑140 (MQ=255)
         cGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAT‑ATGAggt                                                                                                                             >  2:1437774/1‑138 (MQ=255)
         cGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAA‑ATGAGtt                                                                                                                             >  2:738304/1‑140 (MQ=255)
         cGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAT‑ATGAGtt                                                                                                                             >  1:724394/1‑140 (MQ=255)
           ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTGTGGGAAAT‑ATGAGATTa                                                                                                                           >  1:379550/1‑140 (MQ=255)
           ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTa                                                                                                                           <  1:994225/140‑1 (MQ=255)
           ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTa                                                                                                                           >  1:872640/1‑140 (MQ=255)
           ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAT‑ATTAGtttt                                                                                                                           >  1:1681562/1‑139 (MQ=255)
           ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAA‑ATGAGtttt                                                                                                                           >  2:1338264/1‑139 (MQ=255)
           ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAA‑ATGAGTTTa                                                                                                                           >  2:1526124/1‑140 (MQ=255)
           ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAA‑ATGAGTTTa                                                                                                                           >  2:241084/1‑140 (MQ=255)
           ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAT‑ATGAGTTTa                                                                                                                           >  1:281596/1‑140 (MQ=255)
           ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAT‑ATGAGTTTa                                                                                                                           >  1:611763/1‑140 (MQ=255)
           ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGCTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAT‑ATTAGTTTa                                                                                                                           >  2:735406/1‑140 (MQ=255)
            gCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAg                                                                                                                          <  1:77025/140‑1 (MQ=255)
             cTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGc                                                                                                                         <  1:735406/140‑1 (MQ=255)
             cTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGc                                                                                                                         <  1:642857/140‑1 (MQ=255)
              tGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGcc                                                                                                                        <  1:825488/140‑1 (MQ=255)
              ggacAGAAGCGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGcc                                                                                                                        <  1:57803/137‑1 (MQ=255)
               ggCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGccc                                                                                                                       <  1:953003/140‑1 (MQ=255)
               ggCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGccc                                                                                                                       <  1:1765266/140‑1 (MQ=255)
               ggCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGccc                                                                                                                       <  2:961654/140‑1 (MQ=255)
                gCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGcccc                                                                                                                      <  1:1019333/140‑1 (MQ=255)
                 cAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAT‑ATGAGTTTAGccccc                                                                                                                     >  2:77025/1‑140 (MQ=255)
                       cGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAT‑ATGAGTTTAGCCCCCtactta                                                                                                               >  1:1587477/1‑140 (MQ=255)
                         gtgtGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCtacttact                                                                                                             <  1:1374997/140‑1 (MQ=255)
                           gtgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTGTGGGAAAA‑ATGAGTTTAACCCCCttcttacccc                                                                                                           >  1:265194/1‑137 (MQ=255)
                            tgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAT‑ATGAGTTTAGCCCCCCTCTTACTCGa                                                                                                          >  2:461934/1‑140 (MQ=255)
                                gATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCa                                                                                                      <  1:414373/140‑1 (MQ=255)
                                   tCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCaaaa                                                                                                   <  1:68050/140‑1 (MQ=255)
                                    cACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAA‑ATGAGTTTTGCCCCCCACTTTCTCGATGCaaaaa                                                                                                  >  2:1442776/1‑139 (MQ=255)
                                      cGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCa                                                                                                <  2:297277/140‑1 (MQ=255)
                                       ggTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAc                                                                                               <  2:611763/140‑1 (MQ=255)
                                        gTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACg                                                                                              <  1:738304/140‑1 (MQ=255)
                                         tCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGc                                                                                             <  2:1253101/140‑1 (MQ=255)
                                          cAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGct                                                                                            <  1:1589974/140‑1 (MQ=255)
                                          cAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGct                                                                                            <  2:872640/140‑1 (MQ=255)
                                           aaTAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAA‑ATGAGTTTAGCCCCCCACTTTCTCGATGCAAAAGCACGctc                                                                                           >  2:1589974/1‑140 (MQ=255)
                                                aGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGtt                                                                                      <  1:1265806/140‑1 (MQ=255)
                                                  tATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTAt                                                                                    <  1:365176/140‑1 (MQ=255)
                                                   aTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATc                                                                                   >  2:1417874/1‑140 (MQ=255)
                                                     gtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGt                                                                                 >  1:544272/1‑140 (MQ=255)
                                                       ggTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCt                                                                               <  1:1068288/140‑1 (MQ=255)
                                                        gTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAACAA‑ATTGGATTTGCCCCCCACCTTCTCGATTCAAAAACACACGCTGGGTCTCGTCtt                                                                              >  2:1014405/1‑140 (MQ=255)
                                                         ttCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTGCTCGATGCAAAAGCACGCTCTGGTTATCGTCTTg                                                                             <  2:215636/140‑1 (MQ=255)
                                                         ttCGGGTCTTAAAAGTGTAGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAT‑ATGAGTTTAGTCCCCCACTTACTCCATGCAAAAGCACGCCCTGGTTATCGTCTTg                                                                             >  2:1350077/1‑140 (MQ=255)
                                                           cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTTga                                                                           >  1:951747/1‑140 (MQ=255)
                                                            gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAA‑ATGAGTTTAGGCCCCTACTTACTCCATGCAAAAGCACGCCCTGGTTATCGTCTTGgag                                                                          >  2:153047/1‑140 (MQ=255)
                                                               tCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACg                                                                       <  2:281596/140‑1 (MQ=255)
                                                                cTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACgg                                                                      >  1:1556849/1‑140 (MQ=255)
                                                                 ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGa                                                                     <  2:1084887/140‑1 (MQ=255)
                                                                 ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGa                                                                     <  2:1714408/140‑1 (MQ=255)
                                                                 ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGa                                                                     <  1:1417874/140‑1 (MQ=255)
                                                                 ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGa                                                                     <  1:241084/140‑1 (MQ=255)
                                                                   aaaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACa                                                                   >  1:234223/1‑140 (MQ=255)
                                                                    aaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAg                                                                  <  1:304903/140‑1 (MQ=255)
                                                                         gtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTa                                                             <  2:521895/140‑1 (MQ=255)
                                                                            gcgcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGa                                                          <  1:844124/140‑1 (MQ=255)
                                                                             cgcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAA‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGAc                                                         >  1:1599500/1‑140 (MQ=255)
                                                                               cTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGt                                                       >  2:1364800/1‑140 (MQ=255)
                                                                               cTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAT‑ATGAGTTTTGCCCCCCCCCTACTTCATTTAAAAGAACGCTCTGGTTATCGTCTTGGAGACCGACAGGGTTATGaggg                                                       >  2:844124/1‑137 (MQ=255)
                                                                               cTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAA‑ATTAGTTTTGCCCCCCACTTACTCCATGCAAAAACACGCTCTGGTTATCGTCTTGGAGACGGACAAGACTATGACGt                                                       >  2:979017/1‑140 (MQ=255)
                                                                                 tGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTaa                                                     <  2:724394/140‑1 (MQ=255)
                                                                                  gccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGTTGGGAAAT‑ATGAGTTTTGCCCCCAACTTACTCGACTCCCAACCACGCTCTGGCTATTGTCTTGGGGATGGATAAGGTTCTTACGTAAt                                                    >  2:1301896/1‑140 (MQ=255)
                                                                                  gccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAT‑ATGAATTTAGCCCCCCCCTTTATCGGTGCCAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAAAAGGTTATGACGTAAt                                                    >  2:1754239/1‑140 (MQ=255)
                                                                                    cgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATcc                                                  <  2:926531/140‑1 (MQ=255)
                                                                                     gccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCt                                                 <  2:357321/140‑1 (MQ=255)
                                                                                          ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGcgcg                                            <  1:1442776/140‑1 (MQ=255)
                                                                                          ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGcgcg                                            <  1:1350077/140‑1 (MQ=255)
                                                                                          ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGcgcg                                            <  1:882695/140‑1 (MQ=255)
                                                                                          ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGcgcg                                            <  1:361856/140‑1 (MQ=255)
                                                                                            ccATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTCTATGACGTAATCCTGCGCGAt                                          <  1:979017/140‑1 (MQ=255)
                                                                                              aTTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATgg                                        <  2:1681562/140‑1 (MQ=255)
                                                                                                tCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAA‑ATGAGTTTAGCCCCCCACTTACTCGATGCAAAAGCACGCTCTGGTTATTGTCTTGGAGACGGGCAGGGTTATTACGGAATCCTGCGCGGTGGcc                                      >  2:624719/1‑140 (MQ=255)
                                                                                                   ggcaggTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGa                                   <  1:153047/140‑1 (MQ=255)
                                                                                                        gTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTgc                              <  1:1296544/140‑1 (MQ=255)
                                                                                                              cgcAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCAcc                        <  2:1587477/140‑1 (MQ=255)
                                                                                                              cgcAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCAcc                        <  1:1282946/140‑1 (MQ=255)
                                                                                                              cgcAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCAcc                        <  1:1301896/140‑1 (MQ=255)
                                                                                                              cgcAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCAcc                        <  1:1014405/140‑1 (MQ=255)
                                                                                                               gcAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCAccc                       <  1:1364800/140‑1 (MQ=255)
                                                                                                               gcAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCAccc                       <  1:1735609/140‑1 (MQ=255)
                                                                                                               gcAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCG‑CACCCa                      <  1:304743/140‑1 (MQ=255)
                                                                                                                cAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCa                      <  2:1051160/140‑1 (MQ=255)
                                                                                                                 agagCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCAt                     <  2:544272/140‑1 (MQ=255)
                                                                                                                   agCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATgg                   <  1:461934/140‑1 (MQ=255)
                                                                                                                    gCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGt                  <  1:624719/140‑1 (MQ=255)
                                                                                                                     cATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGtt                 <  1:537099/140‑1 (MQ=255)
                                                                                                                      aTTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTa                <  2:379550/140‑1 (MQ=255)
                                                                                                                       ttGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTAt               <  2:1556849/140‑1 (MQ=255)
                                                                                                                               ggggggATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCCCCCATGGTTATCATATggg       >  2:1497777/1‑140 (MQ=255)
                                                                                                                                    tATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTa  >  1:958497/1‑140 (MQ=255)
                                                                                                                                    |                                                                                                                                            
GTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAT‑ATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTA  >  NC_000913/2326318‑2326589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑