BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,662,058	G→T	P25Q (CCG→CAG)	iscR ←	DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,662,058	0	G	T	100.0%	363.3 / NA	101	P25Q (CCG→CAG)	iscR	DNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (50/51); total (50/51)

Only 100 of 102 total aligned reads displayed.

CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTA  >  NC_000913/2661921‑2662190

cATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCtgg                                                                                                                                    >  1:1227081/1‑140 (MQ=255)

 aTCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGc                                                                                                                                   >  2:203716/1‑140 (MQ=255)

  tCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGcc                                                                                                                                  <  1:203716/140‑1 (MQ=255)

  tCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGcc                                                                                                                                  <  1:1664873/140‑1 (MQ=255)

  tCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGcc                                                                                                                                  <  1:1618781/140‑1 (MQ=255)

  tCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGcc                                                                                                                                  <  2:1224700/140‑1 (MQ=255)

    tttGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCg                                                                                                                                >  1:374799/1‑140 (MQ=255)

       gCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCtt                                                                                                                             <  1:976598/140‑1 (MQ=255)

            aCAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCatag                                                                                                                        >  1:1737566/1‑140 (MQ=255)

             cAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCa                                                                                                                           >  2:671039/1‑136 (MQ=255)

             cAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCa                                                                                                                           <  1:671039/136‑1 (MQ=255)

                aTAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGa                                                                                                                    >  1:661440/1‑140 (MQ=255)

                  aaCCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGa                                                                                                                    >  2:1637226/1‑138 (MQ=255)

                  aaCCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGa                                                                                                                    <  1:1637226/138‑1 (MQ=255)

                  aaCCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAgc                                                                                                                  <  1:836994/140‑1 (MQ=255)

                   accaccGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAgcg                                                                                                                 <  2:1227081/140‑1 (MQ=255)

                   accaccGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAgcg                                                                                                                 <  2:1600630/140‑1 (MQ=255)

                   accaccGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAgcg                                                                                                                 <  1:942890/140‑1 (MQ=255)

                    ccaccGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAgcgc                                                                                                                <  2:166142/140‑1 (MQ=255)

                        cGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACg                                                                                                            >  1:39230/1‑140 (MQ=255)

                        cGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACg                                                                                                            >  1:505706/1‑140 (MQ=255)

                        cGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACg                                                                                                            >  2:128913/1‑140 (MQ=255)

                        cGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACg                                                                                                            >  2:1089704/1‑140 (MQ=255)

                        cGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACg                                                                                                            >  2:838042/1‑140 (MQ=255)

                          ccTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTc                                                                                                          >  2:1503181/1‑140 (MQ=255)

                          ccTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTc                                                                                                          >  2:358039/1‑140 (MQ=255)

                          ccTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTc                                                                                                          >  2:1169507/1‑140 (MQ=255)

                          ccTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTc                                                                                                          >  1:792980/1‑140 (MQ=255)

                          ccTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTc                                                                                                          <  1:381080/140‑1 (MQ=255)

                               tCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCa                                                                                                     >  2:1387689/1‑140 (MQ=255)

                               tCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCa                                                                                                     >  2:85607/1‑140 (MQ=255)

                                   cGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGc                                                                                                 >  2:1256916/1‑140 (MQ=255)

                                        gCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCa                                                                                            <  2:1742420/140‑1 (MQ=255)

                                        gCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCa                                                                                            <  1:1387689/140‑1 (MQ=255)

                                        gCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCa                                                                                            >  2:633861/1‑140 (MQ=255)

                                        gCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCa                                                                                            >  2:1516477/1‑140 (MQ=255)

                                        gCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCa                                                                                            <  2:188250/140‑1 (MQ=255)

                                        gCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCa                                                                                            >  1:1768987/1‑140 (MQ=255)

                                         cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCAc                                                                                           >  2:622778/1‑140 (MQ=255)

                                         cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCAc                                                                                           >  2:1249116/1‑140 (MQ=255)

                                             aaaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCa                                                                                       <  1:415289/140‑1 (MQ=255)

                                                  aGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAgcg                                                                                  <  1:85607/140‑1 (MQ=255)

                                                  aGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAgcg                                                                                  <  2:661440/140‑1 (MQ=255)

                                                   gACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAgcgc                                                                                 <  1:1516477/140‑1 (MQ=255)

                                                   gACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAgcgc                                                                                 >  1:470620/1‑140 (MQ=255)

                                                    aCCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGcc                                                                                <  1:1160106/140‑1 (MQ=255)

                                                    aCCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGcc                                                                                >  1:284403/1‑140 (MQ=255)

                                                    aCCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCACAACGTCAAGCATTGCGGTCACGGCATAGCGcc                                                                                >  2:776848/1‑140 (MQ=255)

                                                              cGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGt                                                                      <  1:925329/140‑1 (MQ=255)

                                                              cGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGt                                                                      <  1:1503181/140‑1 (MQ=255)

                                                              cGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGt                                                                      <  2:694714/140‑1 (MQ=255)

                                                              cGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGt                                                                      <  1:358039/140‑1 (MQ=255)

                                                              cGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGt                                                                      <  2:1768987/140‑1 (MQ=255)

                                                                  gACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGt                                                                  <  2:219445/140‑1 (MQ=255)

                                                                  gACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGt                                                                  >  2:872638/1‑140 (MQ=255)

                                                                   aCGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGtc                                                                 >  1:292081/1‑140 (MQ=255)

                                                                   aCGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGtc                                                                 >  1:1263561/1‑140 (MQ=255)

                                                                   aCGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGtc                                                                 >  2:1564255/1‑140 (MQ=255)

                                                                   aCGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGtc                                                                 >  1:1520531/1‑140 (MQ=255)

                                                                   aCGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGtc                                                                 <  2:1520531/140‑1 (MQ=255)

                                                                   aCGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGtc                                                                 >  2:781967/1‑140 (MQ=255)

                                                                   aCGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGtc                                                                 >  1:186738/1‑140 (MQ=255)

                                                                     gggAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGtctc                                                               <  1:128913/140‑1 (MQ=255)

                                                                         aaaCAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCATAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGt                                                           <  2:1737566/140‑1 (MQ=255)

                                                                           aCAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCt                                                         <  1:1169507/140‑1 (MQ=255)

                                                                             aGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTa                                                       >  1:1632494/1‑140 (MQ=255)

                                                                               ttgttCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACt                                                     >  1:696052/1‑140 (MQ=255)

                                                                                tgttCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACtt                                                    <  1:536024/140‑1 (MQ=255)

                                                                                       gATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGtctc                                                               <  1:653188/122‑1 (MQ=255)

                                                                                       gATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGtctc                                                               >  2:653188/1‑122 (MQ=255)

                                                                                            gAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTc                                        >  1:204130/1‑140 (MQ=255)

                                                                                             aaaGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCg                                       >  1:1035033/1‑140 (MQ=255)

                                                                                               aGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTc                                              >  2:1440793/1‑131 (MQ=255)

                                                                                               aGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTc                                              <  1:1440793/131‑1 (MQ=255)

                                                                                                 ggAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGcccc                                   >  1:454874/1‑140 (MQ=255)

                                                                                                  gAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACt                                         >  2:775566/1‑133 (MQ=255)

                                                                                                  gAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACt                                         <  1:775566/133‑1 (MQ=255)

                                                                                                    aaTTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCACGTCTTACTTCACCTCAAACTCGCCCCTGc                                >  1:377088/1‑140 (MQ=255)

                                                                                                     aTTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGcc                               <  1:633861/140‑1 (MQ=255)

                                                                                                     aTTCCCTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGcc                               <  1:1089704/140‑1 (MQ=255)

                                                                                                         ccTGACGTTCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCggg                           <  2:792980/140‑1 (MQ=255)

                                                                                                                 tCGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTa                   <  2:204130/140‑1 (MQ=255)

                                                                                                                  cGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTAt                  >  1:607284/1‑140 (MQ=255)

                                                                                                                  cGGAAATATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTAt                  >  2:737487/1‑140 (MQ=255)

                                                                                                                     aaaTATCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGt               <  2:1035033/140‑1 (MQ=255)

                                                                                                                       atatCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTaa             <  1:663616/140‑1 (MQ=255)

                                                                                                                        tatCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTaaa            <  2:186738/140‑1 (MQ=255)

                                                                                                                          tCAGCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAg          <  1:1564255/140‑1 (MQ=255)

                                                                                                                             gCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTgg       >  1:1002140/1‑140 (MQ=255)

                                                                                                                             gCCAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTgg       >  1:1470053/1‑140 (MQ=255)

                                                                                                                              ccAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTggg      >  2:206046/1‑140 (MQ=255)

                                                                                                                              ccAACGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTggg      >  2:1334909/1‑140 (MQ=255)

                                                                                                                                aaCGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTggggg    <  2:39230/140‑1 (MQ=255)

                                                                                                                                aaCGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTggggg    <  1:737487/140‑1 (MQ=255)

                                                                                                                                  cGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTggggg    <  1:1176069/138‑1 (MQ=255)

                                                                                                                                  cGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTggggg    >  2:1176069/1‑138 (MQ=255)

                                                                                                                                  cGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTa  <  1:464224/140‑1 (MQ=255)

                                                                                                                                  cGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTa  <  1:781967/140‑1 (MQ=255)

                                                                                                                                  cGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTa  <  1:872638/140‑1 (MQ=255)

                                                                                                                                  cGGTACCTGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACAGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTa  <  2:505706/140‑1 (MQ=255)

CATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTA  >  NC_000913/2661921‑2662190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`