BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,495	C→A	A61A (GCC→GCA)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,495	0	C	A	99.1%	368.1 / NA	105	A61A (GCC→GCA)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (54/50); minor base G (0/1); total (54/51)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

GCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAG  >  NC_000913/3041366‑3041631

gctgacgctgaTGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACg                                                                                                                                >  1:1287090/1‑140 (MQ=255)

 ctgacgctgaTGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGc                                                                                                                               <  2:217553/140‑1 (MQ=255)

   gacgctgaTGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCCCCTGCTCGCCGCGCATTGCGACGCCa                                                                                                                             <  2:919512/140‑1 (MQ=255)

   gacgctgaTGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCa                                                                                                                             <  2:1350951/140‑1 (MQ=255)

   gacgctgaTGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCa                                                                                                                             <  2:699706/140‑1 (MQ=255)

   gacgctgaTGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCa                                                                                                                             <  2:512933/140‑1 (MQ=255)

   gacgctgaTGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCa                                                                                                                             <  2:1774500/140‑1 (MQ=255)

   gacgctgaTGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCa                                                                                                                             <  2:59327/140‑1 (MQ=255)

   gacgctgaTGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCa                                                                                                                             <  1:1463506/140‑1 (MQ=255)

           tgaCGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTaaa                                                                                                                     <  1:149280/140‑1 (MQ=255)

            gaCGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTaaaa                                                                                                                    <  1:1417166/140‑1 (MQ=255)

              cGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAAtg                                                                                                                  >  2:454097/1‑140 (MQ=255)

              cGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAAtg                                                                                                                  <  1:245261/140‑1 (MQ=255)

              cGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAAtg                                                                                                                  >  1:1042807/1‑140 (MQ=255)

               gCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAAtgt                                                                                                                 >  1:1138456/1‑140 (MQ=255)

                  tgatgaCTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGa                                                                                                              >  2:1643929/1‑140 (MQ=255)

                  tgatgaCTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGa                                                                                                              >  1:145013/1‑140 (MQ=255)

                  tgatgaCTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTAAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGa                                                                                                              >  2:1168521/1‑140 (MQ=255)

                   gatgaCTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAg                                                                                                             <  2:421183/140‑1 (MQ=255)

                   gatgaCTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAg                                                                                                             <  2:1287090/140‑1 (MQ=255)

                   gatgaCTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAg                                                                                                             <  1:1182797/140‑1 (MQ=255)

                   gatgaCTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAg                                                                                                             <  2:1086216/140‑1 (MQ=255)

                    atgaCTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGc                                                                                                            >  2:1744613/1‑140 (MQ=255)

                        cTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTTGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAAtt                                                                                                        <  1:579301/140‑1 (MQ=255)

                        cTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAAtt                                                                                                        <  2:623134/140‑1 (MQ=255)

                        cTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAAtt                                                                                                        <  2:698935/140‑1 (MQ=255)

                           ggCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTAc                                                                                                     >  2:1272303/1‑140 (MQ=255)

                             cgcTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGt                                                                                                   >  2:1427044/1‑140 (MQ=255)

                                  gCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGtt                                                                                              >  2:1101330/1‑140 (MQ=255)

                                             tGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCg                                                                                   >  2:933779/1‑140 (MQ=255)

                                                cgcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATg                                                                                >  2:57747/1‑140 (MQ=255)

                                                cgcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATg                                                                                >  2:1357661/1‑140 (MQ=255)

                                                 gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTTGAGCAATTTACGTCTGTTCCGCGACGGCGATgg                                                                               >  2:581904/1‑140 (MQ=255)

                                                 gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATgg                                                                               >  2:512369/1‑140 (MQ=255)

                                                 gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATgg                                                                               >  2:1109858/1‑140 (MQ=255)

                                                 gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATgg                                                                               >  2:727148/1‑140 (MQ=255)

                                                    gACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCtt                                                                            >  1:501873/1‑140 (MQ=255)

                                                         cGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCAt                                                                       <  1:781674/140‑1 (MQ=255)

                                                         cGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCC‑AAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATg                                                                      >  2:233863/1‑140 (MQ=255)

                                                                      cAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGgcg                                                          <  1:933779/140‑1 (MQ=255)

                                                                       aGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGgcgc                                                         <  1:602339/140‑1 (MQ=255)

                                                                        gggTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCa                                                        >  1:624980/1‑140 (MQ=255)

                                                                         ggTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAg                                                       <  2:817670/140‑1 (MQ=255)

                                                                         ggTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAg                                                       <  1:727148/140‑1 (MQ=255)

                                                                         ggTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAg                                                       <  1:1109858/140‑1 (MQ=255)

                                                                         ggTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAg                                                       <  1:1455607/140‑1 (MQ=255)

                                                                         ggTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAg                                                       <  1:1767142/140‑1 (MQ=255)

                                                                         ggTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAg                                                       >  1:500401/1‑140 (MQ=255)

                                                                          gTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGGTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAgc                                                      >  2:1185264/1‑140 (MQ=255)

                                                                          gTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAgc                                                      <  1:812899/140‑1 (MQ=255)

                                                                          gTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAgc                                                      >  1:1594405/1‑140 (MQ=255)

                                                                          gTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAgc                                                      >  2:125820/1‑140 (MQ=255)

                                                                           tCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAgcg                                                     >  1:823780/1‑140 (MQ=255)

                                                                             aGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAgcgtt                                                   >  2:1675432/1‑139 (MQ=255)

                                                                             aGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAgcgtg                                                   >  2:1760068/1‑140 (MQ=255)

                                                                              ggTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAgcgtgc                                                  >  1:610488/1‑140 (MQ=255)

                                                                              ggTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAgcgtgc                                                  >  1:83321/1‑140 (MQ=255)

                                                                                 gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGcgtgcgtg                                               <  1:1427044/140‑1 (MQ=255)

                                                                                   cagcagATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGaa                                             >  1:1093865/1‑140 (MQ=255)

                                                                                   cagcagATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGaa                                             >  2:957954/1‑140 (MQ=255)

                                                                                         aTGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAg                                       >  1:985916/1‑140 (MQ=255)

                                                                                          tGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGc                                      <  2:610488/140‑1 (MQ=255)

                                                                                              aGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGctgac                                  >  2:643835/1‑140 (MQ=255)

                                                                                              aGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGctgac                                  >  1:123773/1‑140 (MQ=255)

                                                                                                ccAGATGGCAGAAGATCAGTACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGctgactg                                >  1:594834/1‑140 (MQ=255)

                                                                                                 cAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGctgactga                               <  1:1744613/140‑1 (MQ=255)

                                                                                                 cAGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGctgactga                               <  2:1594405/140‑1 (MQ=255)

                                                                                                  aGATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGactgaa                              <  2:1258747/140‑1 (MQ=255)

                                                                                                   gATGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGactgaac                             >  2:1338120/1‑140 (MQ=255)

                                                                                                    aTGGCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAG‑AATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGactgaactg                           >  2:625542/1‑140 (MQ=255)

                                                                                                       gCAGAAGATCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGActgaactgaa                         <  2:83321/140‑1 (MQ=255)

                                                                                                            agaTCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAat                    <  2:624980/140‑1 (MQ=255)

                                                                                                              aTCAGCACCTGCTCGCCGCACACTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACATCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAatat                  >  1:767120/1‑140 (MQ=255)

                                                                                                               tCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATg                 <  1:1623601/140‑1 (MQ=255)

                                                                                                               tCAGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATg                 <  1:1101330/140‑1 (MQ=255)

                                                                                                                 aGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCg               <  1:1258959/140‑1 (MQ=255)

                                                                                                                 aGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCg               >  1:1518161/1‑140 (MQ=255)

                                                                                                                 aGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCg               >  1:1537732/1‑140 (MQ=255)

                                                                                                                 aGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCg               <  2:1368716/140‑1 (MQ=255)

                                                                                                                 aGCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCg               >  2:876809/1‑140 (MQ=255)

                                                                                                                  gCACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAG‑AATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGt             <  1:625542/140‑1 (MQ=255)

                                                                                                                   cACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGt             <  2:423432/140‑1 (MQ=255)

                                                                                                                   cACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGt             <  1:1301115/140‑1 (MQ=255)

                                                                                                                   cACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGt             <  1:1272303/140‑1 (MQ=255)

                                                                                                                   cACCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGt             <  2:1042807/140‑1 (MQ=255)

                                                                                                                   cACCTGCTCGCCGCACACTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACATCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGt             <  2:767120/140‑1 (MQ=255)

                                                                                                                    aCCTGGTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTa            <  1:1185264/140‑1 (MQ=255)

                                                                                                                    aCCTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTa            <  1:125820/140‑1 (MQ=255)

                                                                                                                     ccTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGGAt           >  2:779464/1‑140 (MQ=255)

                                                                                                                      cTGCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTAtt          <  2:1537732/140‑1 (MQ=255)

                                                                                                                        gCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATtct        >  1:1075288/1‑140 (MQ=255)

                                                                                                                        gCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATtct        <  1:1357661/140‑1 (MQ=255)

                                                                                                                        gCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATtct        >  1:1002197/1‑140 (MQ=255)

                                                                                                                        gCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATtct        >  1:1642987/1‑140 (MQ=255)

                                                                                                                        gCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATtct        >  1:222810/1‑140 (MQ=255)

                                                                                                                        gCTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATtct        >  1:380887/1‑140 (MQ=255)

                                                                                                                         cTCGCCGCACATTGCGACGCCAAAGGTAAAATGTTGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATtctc       <  1:581904/140‑1 (MQ=255)

                                                                                                                         cTCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATtctc       >  1:482452/1‑140 (MQ=255)

                                                                                                                          tCGCCGCACATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTctct      <  1:1338120/140‑1 (MQ=255)

                                                                                                                              cgcaCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAg  >  1:1077817/1‑140 (MQ=255)

GCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAG  >  NC_000913/3041366‑3041631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`