Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,734,264	G→T	G182V (GGC→GTC)	xylH →	xylose ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,734,264	0	G	T	100.0%	316.4 / NA	90	G182V (GGC→GTC)	xylH	xylose ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (55/35);  total (55/35)

CCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCGCTATCA  >  NC_000913/3734128‑3734398                                                                                                                                          |                                                                                                                                       ccTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCtt                                                                                                                                     >  1:293853/1‑140 (MQ=255)  cTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTtc                                                                                                                                    >  2:448925/1‑140 (MQ=255)  cTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTtc                                                                                                                                    >  2:611820/1‑140 (MQ=255)   ttCTTTTTTTGCCCCCCCCGCGGGTATGTTGGCTTTCCGGGGCATACTCATGGGCATCACCAACGGCACGACTGTCCCCCCCCCCCGCCCCGAGTTGCCAAAAATTGGGCAAG‑GCTTTCTCCCCGCCAGTACCGTCTtca                                                                                                                                   <  2:760458/140‑1 (MQ=255)            tGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGgcg                                                                                                                          >  1:760458/1‑140 (MQ=255)               ccccccCGGGGGCTTTTTGGCATTTCGGGCCATACTCTTGGGCATCACCAACGGCACGACTGTCCCCCCCCCCAGCGCCGCGATGTAAAAAATTGGGCAAA‑GCTATCCCCCCGCCAGTACCGTCTTCATCATTGgcgcgc                                                                                                                       <  2:506268/138‑1 (MQ=255)                cccccCGCGGGCTTTTTGGCTTTTGGCGGCATCCTCTTTGGCACCACCACCGGCACGTCTGTCCCCCCCCCCAGCGCCGCGATGTCAAAATTGGGGCAAA‑GCTATCCCCCCGCCAGTACCGTCTTCATCATTGGCGCGCt                                                                                                                      <  2:1254093/139‑1 (MQ=255)                ccccTCGCGGGGTTGTTGCGGTTTCGCGGCATCACCAGTGCAATCCCAAACGGCCGGGTTTAAGCCCCCCCCGGCGCCGAGATCACAAAAAGGGGGAAAT‑CCTCTCCCCCCGCCAGTACCGTCTTCATCATTGGCGCGCt                                                                                                                      <  2:961812/139‑1 (MQ=255)                aCCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCt                                                                                                                      >  2:1223062/1‑140 (MQ=255)                aCCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCt                                                                                                                      >  2:1420744/1‑140 (MQ=255)                aCCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCt                                                                                                                      >  2:503803/1‑140 (MQ=255)                                tGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCtttt                                                                                                      >  1:376855/1‑140 (MQ=255)                                    aTTTCGCGGCATACTCATTGGCATC‑CCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTgttgg                                                                                                 >  1:615499/1‑140 (MQ=255)                                              atccTCATTGGCATCCCCAGCGCCACGACTGTACCCCCCCCCAGCGCCGCGATGTCACAAATGGGGCAAA‑GCTCCCCCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATg                                                                                        <  2:995771/137‑1 (MQ=255)                                                ccTCATGGGCACCCCCACCGCCACGACTGTCCCCCCCCCCAGCGCCGGGATGCCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGgc                                                                                      <  2:954279/139‑1 (MQ=255)                                                    aTTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCgg                                                                                  >  2:219315/1‑140 (MQ=255)                                                       ggCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGgaag                                                                               >  1:954279/1‑140 (MQ=255)                                                       ggCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGgaag                                                                               >  2:762434/1‑140 (MQ=255)                                                         cATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGgaagaa                                                                             >  1:1137401/1‑140 (MQ=255)                                                          aTCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAAt                                                                            >  1:465103/1‑140 (MQ=255)                                                           cccccAACGGCCCGACTGTCCCCCCCCCCAGCGCCGCGATGTCAAAAATTGGGCAAA‑GCTATCCCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATg                                                                           <  2:465103/137‑1 (MQ=255)                                                            cACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATgc                                                                          >  1:171712/1‑140 (MQ=255)                                                            cACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATgc                                                                          >  1:832148/1‑140 (MQ=255)                                                            cACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATgc                                                                          >  2:1656009/1‑140 (MQ=255)                                                            cACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATgc                                                                          >  1:409581/1‑140 (MQ=255)                                                            cACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATgc                                                                          >  1:561163/1‑140 (MQ=255)                                                                 aCGGCACGATTGTCCCCCCCCCCGGCGCCGCGATTTCAAAATTGGGGAAAA‑TCTTTCCCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGt                                                                     <  2:1069137/140‑1 (MQ=255)                                                                 aCGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGt                                                                     >  2:360234/1‑140 (MQ=255)                                                                 aCGGCACGACTGTACCCCCCCCCAGCGCCGGGATGTCACAAATGGGGAAAA‑GCTATCCCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGt                                                                     <  1:448925/140‑1 (MQ=255)                                                                  cGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTc                                                                    >  2:273630/1‑140 (MQ=255)                                                                  cGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTc                                                                    >  1:562413/1‑140 (MQ=255)                                                                       cGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCt                                                               >  2:1545219/1‑140 (MQ=255)                                                                             tATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGt                                                         >  1:83965/1‑140 (MQ=255)                                                                             tATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGt                                                         >  1:329296/1‑140 (MQ=255)                                                                             tATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGt                                                         >  1:1318610/1‑140 (MQ=255)                                                                              aTCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGtt                                                        >  1:1185582/1‑140 (MQ=255)                                                                                 ccccccCAGCGCCGCGTTGTAAAAAATGGGGAAAA‑GCTATCCCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTAc                                                     <  2:409581/140‑1 (MQ=255)                                                                                 ccccccCAGCCCCGCATTGTCACAAATTGGGCAAA‑GTTCCCCCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTAc                                                     <  1:503803/140‑1 (MQ=255)                                                                                 ccccccCACCGCCGGGATGTAACAAATTGGACAAA‑GCTATCCCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTAc                                                     <  2:561163/140‑1 (MQ=255)                                                                                 ccccACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTAc                                                     <  1:1420744/140‑1 (MQ=255)                                                                                  ccccccAGCGCCGCGTTGACAAAATTGGGGCAAA‑GCTCTCCCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACa                                                    <  2:606099/140‑1 (MQ=255)                                                                                   ccaccaGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAg                                                   >  1:729423/1‑140 (MQ=255)                                                                                    caccaGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGt                                                  >  1:1093824/1‑140 (MQ=255)                                                                                    caccaGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGt                                                  >  1:1740731/1‑140 (MQ=255)                                                                                    caccaGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGt                                                  >  1:606099/1‑140 (MQ=255)                                                                                     accaGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGtc                                                 >  2:1416654/1‑140 (MQ=255)                                                                                       caGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTAATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGtctc                                               >  2:1016353/1‑140 (MQ=255)                                                                                         gcgcCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCg                                             >  2:851397/1‑140 (MQ=255)                                                                                         gcgcCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCg                                             >  2:1474524/1‑140 (MQ=255)                                                                                          cgccgcGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCgg                                            >  1:1076262/1‑140 (MQ=255)                                                                                          cgccgcGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCgg                                            >  1:711527/1‑140 (MQ=255)                                                                                          cgccgcGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCgg                                            >  1:735203/1‑140 (MQ=255)                                                                                           gccgcGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGc                                           >  1:797784/1‑140 (MQ=255)                                                                                           cccgcGATGTCAAAAATGGGGAAAA‑GCTCTCCCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGc                                           <  2:653445/139‑1 (MQ=255)                                                                                            ccgcGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGcc                                          >  1:653445/1‑140 (MQ=255)                                                                                            ccgcGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGcc                                          >  1:494828/1‑140 (MQ=255)                                                                                               cgATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCctct                                       >  1:1430301/1‑140 (MQ=255)                                                                                                gATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTa                                      >  1:1691828/1‑140 (MQ=255)                                                                                                 aTGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGTTTTACAGTCTCCGGCCTCTAc                                     >  1:1077486/1‑140 (MQ=255)                                                                                                  atgtaaaaaTTGGGGAAAGGCTTTCCCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTAc                                     <  2:1099100/134‑1 (MQ=255)                                                                                                       aaaaTTGGGAAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCagt                               <  1:1633641/139‑1 (MQ=255)                                                                                                           tgggacAAA‑GCTATCCCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGTTTTACAGTCTCCGGCCTCTACCGCAGTAgtc                           <  2:1077486/135‑1 (MQ=255)                                                                                                            tGGGCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAgtcg                          >  1:555934/1‑140 (MQ=255)                                                                                                             gggCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAgtcgg                         >  2:146234/1‑140 (MQ=255)                                                                                                              ggCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAgtcggt                        >  1:798564/1‑140 (MQ=255)                                                                                                              ggCAAA‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAgtcggt                        >  1:124953/1‑140 (MQ=255)                                                                                                                 aaa‑GCTTCCCCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGc                     <  2:1093824/140‑1 (MQ=255)                                                                                                                 aaa‑GCTCCCCCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGc                     <  2:1185582/140‑1 (MQ=255)                                                                                                                 aaa‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGc                     <  1:273630/140‑1 (MQ=255)                                                                                                                   a‑GCTATCTCCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCa                   >  2:775060/1‑140 (MQ=255)                                                                                                                         tctcCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCtt              >  1:813482/1‑140 (MQ=255)                                                                                                                          ctcCCCGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCttt             <  1:1223062/140‑1 (MQ=255)                                                                                                                           cccccGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTa            <  1:1416654/139‑1 (MQ=255)                                                                                                                               cGCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCg        <  1:1656009/140‑1 (MQ=255)                                                                                                                                gCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCGc       >  1:1744237/1‑140 (MQ=255)                                                                                                                                gCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCGc       >  1:110171/1‑140 (MQ=255)                                                                                                                                gCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCGc       >  2:576547/1‑140 (MQ=255)                                                                                                                                gCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCGc       <  2:1740731/140‑1 (MQ=255)                                                                                                                                gCCAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCGc       >  1:335559/1‑140 (MQ=255)                                                                                                                                  cAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCGCTa     <  2:1374929/140‑1 (MQ=255)                                                                                                                                  cAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCGCTa     <  2:1137401/140‑1 (MQ=255)                                                                                                                                  cAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCGCTa     <  2:124953/140‑1 (MQ=255)                                                                                                                                  cAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCGCTa     <  2:711527/140‑1 (MQ=255)                                                                                                                                  cAGTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCGCTa     <  2:735203/140‑1 (MQ=255)                                                                                                                                    gTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCGCTatc   <  1:1474524/140‑1 (MQ=255)                                                                                                                                    gTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCGCTatc   <  2:562413/140‑1 (MQ=255)                                                                                                                                    gTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCGCTatc   <  2:1318164/140‑1 (MQ=255)                                                                                                                                    gTACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCGCTatc   <  2:494828/140‑1 (MQ=255)                                                                                                                                     tACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCGCTatca  <  2:555934/140‑1 (MQ=255)                                                                                                                                     tACCGTCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCGCTatca  >  2:1123698/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       CCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAA‑GCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACCGCAGTAGTCGGTCGCCAGGCTTTAACCGCTATCA  >  NC_000913/3734128‑3734398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑