Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,747,487	C→A	P135Q (CCG→CAG)	lyxK →	L‑xylulose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,747,487	0	C	A	97.6%	283.7 / ‑1.3	82	P135Q (CCG→CAG)	lyxK	L‑xylulose kinase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (36/44);  total (37/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCAAAGGCTGTGAAGAGAGCAATATTTCC  >  NC_000913/3747350‑3747620                                                                                                                                          |                                                                                                                                      tAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAgg                                                                                                                                     >  1:1506256/1‑140 (MQ=255)    aaaCGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGgtgt                                                                                                                                  <  1:901076/140‑1 (MQ=255)              ccGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACg                                                                                                                        <  2:535902/140‑1 (MQ=255)              ccGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACg                                                                                                                        <  2:248388/140‑1 (MQ=255)               cGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACgc                                                                                                                       <  1:68926/140‑1 (MQ=255)                gCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACgct                                                                                                                      <  2:49594/140‑1 (MQ=255)                       aaTGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGaa                                                                                                               <  2:275050/140‑1 (MQ=255)                         tGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAag                                                                                                             >  1:1005979/1‑140 (MQ=255)                           cTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAagag                                                                                                           >  1:222839/1‑140 (MQ=255)                             aTTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCa                                                                                                         <  2:326349/140‑1 (MQ=255)                                ttGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGa                                                                                                      >  1:832686/1‑140 (MQ=255)                                 tGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGaa                                                                                                     >  2:1307066/1‑140 (MQ=255)                                   tCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAAcc                                                                                                   >  1:102023/1‑140 (MQ=255)                                   tCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAAcc                                                                                                   >  2:743284/1‑140 (MQ=255)                                   tCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAAcc                                                                                                   >  2:714148/1‑140 (MQ=255)                                        ggACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAAc                                                                                              >  2:1761989/1‑140 (MQ=255)                                        ggACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAAc                                                                                              >  1:526773/1‑140 (MQ=255)                                        ggACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAAc                                                                                              >  1:521639/1‑140 (MQ=255)                                        ggACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAAc                                                                                              >  1:225174/1‑140 (MQ=255)                                            cgccgGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTa                                                                                          <  1:1296942/140‑1 (MQ=255)                                             gccgGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTAc                                                                                         >  1:592806/1‑140 (MQ=255)                                              ccgGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTAcg                                                                                        <  1:958694/140‑1 (MQ=255)                                              ccgGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTAcg                                                                                        <  1:692542/140‑1 (MQ=255)                                              ccgGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTAcg                                                                                        <  1:1194984/140‑1 (MQ=255)                                              ccgGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTAcg                                                                                        <  2:237553/140‑1 (MQ=255)                                               cgGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTAcgc                                                                                       >  1:290271/1‑140 (MQ=255)                                                 ggCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGCCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACgcgc                                                                                     >  1:1072368/1‑140 (MQ=255)                                                  gCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCa                                                                                    <  1:1449643/140‑1 (MQ=255)                                                      tGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCCTCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAAtt                                                                                >  2:1309302/1‑140 (MQ=255)                                                       ggAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTg                                                                               <  2:290271/140‑1 (MQ=255)                                                        gAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTgg                                                                              <  2:225174/140‑1 (MQ=255)                                                        gAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTgg                                                                              <  2:526773/140‑1 (MQ=255)                                                                    cgCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGtgatgat                                                                  >  1:1248223/1‑140 (MQ=255)                                                                       tGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGAc                                                               <  2:1559355/140‑1 (MQ=255)                                                                        ggCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACg                                                              <  1:1761989/140‑1 (MQ=255)                                                                        ggCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACg                                                              <  2:409292/140‑1 (MQ=255)                                                                            ggAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACg                                                          <  2:768497/140‑1 (MQ=255)                                                                                gATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTa                                                      >  2:146494/1‑140 (MQ=255)                                                                                 aTGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTAc                                                     <  2:1248223/140‑1 (MQ=255)                                                                                      aTCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTgcg                                                <  1:146494/140‑1 (MQ=255)                                                                                       tCCCGGATAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTgcgc                                               <  1:307385/140‑1 (MQ=255)                                                                                       tCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTgcgc                                               <  1:1407394/140‑1 (MQ=255)                                                                                       tCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTgcgc                                               <  2:832686/140‑1 (MQ=255)                                                                                       tCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTgcgc                                               >  1:1599033/1‑140 (MQ=255)                                                                                       tCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTATGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTgcgc                                               >  1:755111/1‑140 (MQ=255)                                                                                        cccGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCt                                              <  1:1070591/140‑1 (MQ=255)                                                                                        cccGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCt                                              >  1:1205341/1‑140 (MQ=255)                                                                                        cccGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCt                                              <  2:1506256/140‑1 (MQ=255)                                                                                         ccGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTCCGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTg                                             <  1:1309302/140‑1 (MQ=255)                                                                                           ggAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCATGACTACCTGCGCTGgt                                           >  2:1740834/1‑140 (MQ=255)                                                                                             aaaaaCTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGgtgt                                         >  1:1184472/1‑140 (MQ=255)                                                                                                 aCTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTgcgc                                               >  1:1487930/1‑130 (MQ=255)                                                                                                 aCTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTgcgc                                               <  2:1487930/130‑1 (MQ=255)                                                                                                  ctctACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAAc                                    >  1:646935/1‑140 (MQ=255)                                                                                                  ctctACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAAc                                    <  1:1506151/140‑1 (MQ=255)                                                                                                   tctACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAAcg                                   <  2:646935/140‑2 (MQ=255)                                                                                                    ctACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTg                                  <  2:1599033/140‑1 (MQ=255)                                                                                                    ctACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTg                                  <  1:241212/140‑1 (MQ=255)                                                                                                       cccGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCg                               >  1:1504132/1‑140 (MQ=255)                                                                                                       cccGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCg                               >  2:357166/1‑140 (MQ=255)                                                                                                         cGCTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTc                             <  2:222839/140‑1 (MQ=255)                                                                                                           cTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCaa                           <  1:1307066/140‑1 (MQ=255)                                                                                                           cTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCaa                           <  2:1543656/140‑1 (MQ=255)                                                                                                           cTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCaa                           <  2:592806/140‑1 (MQ=255)                                                                                                           cTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCaa                           <  2:102023/140‑1 (MQ=255)                                                                                                           cTGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCaa                           <  2:1184472/140‑1 (MQ=255)                                                                                                            tGACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCaaa                          >  2:1378150/1‑140 (MQ=255)                                                                                                             gACCCGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCATGACTACCTGCGCTGGTGTTTAACTGGCGTCAAAg                         <  1:1740834/140‑1 (MQ=255)                                                                                                                ccGACAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCAAAGGCt                      <  1:1057344/140‑1 (MQ=255)                                                                                                                    cAAACCTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCAAAGGCTGTGa                  <  2:1588615/140‑1 (MQ=255)                                                                                                                        ccTTGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCAAAGGCTGTGAagag              >  2:232315/1‑140 (MQ=255)                                                                                                                          ttGTGGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTAAGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCAAAGGCTGTGAagagag            <  1:966367/140‑1 (MQ=255)                                                                                                                             tgGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCAAAGGCTGTGAAGAGAGCaa         <  2:359338/140‑1 (MQ=255)                                                                                                                             tgGACCGGGCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCAAAGGCTGTGAAGAGAGCaa         >  1:1057248/1‑140 (MQ=255)                                                                                                                                   gggCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCAAAGGCTGTGAAGAGAGCAATATTTc   >  2:1002906/1‑140 (MQ=255)                                                                                                                                   gggCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCAAAGGCTGTGAAGAGAGCAATATTTc   <  1:743284/140‑1 (MQ=255)                                                                                                                                   gggCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCAAAGGCTGTGAAGAGAGCAATATTTc   >  1:754898/1‑140 (MQ=255)                                                                                                                                   gggCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCAAAGGCTGTGAAGAGAGCAATATTTc   >  2:1077993/1‑140 (MQ=255)                                                                                                                                   gggCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCATCAAAGGCTGTGAAGAGAGCAATATTTc   <  1:1400987/140‑1 (MQ=255)                                                                                                                                    ggCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCAAAGGCTGTGAAGAGAGCAATATTTcc  >  1:499148/1‑140 (MQ=255)                                                                                                                                    ggCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCAAAGGCTGTGAAGAGAGCAATATTTcc  >  2:1520488/1‑140 (MQ=255)                                                                                                                                    ggCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCAAAGGCTGTGAAGAGAGCAATATTTcc  >  2:690382/1‑140 (MQ=255)                                                                                                                                    ggCATCAGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCAAAGGCTGTGAAGAGAGCAATATTTcc  >  1:1034670/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      TAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGACGCACGACTACCTGCGCTGGTGTTTAACTGGCGTCAAAGGCTGTGAAGAGAGCAATATTTCC  >  NC_000913/3747350‑3747620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑