Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,632,544 G→T E604D (GAG→GAT)  slt → soluble lytic murein transglycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,632,5440GT100.0% 495.8 / NA 139E604D (GAG→GAT) sltsoluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (62/77);  total (62/77)

Only 100 of 141 total aligned reads displayed.

GTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACG  >  NC_000913/4632408‑4632678
                                                                                                                                        |                                                                                                                                      
gTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGt                                                                                                                                     >  1:758546/1‑140 (MQ=255)
        cAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCAtt                                                                                                                             >  1:815736/1‑140 (MQ=255)
           tatGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATtctc                                                                                                                          >  2:490522/1‑140 (MQ=255)
           tatGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATtctc                                                                                                                          >  2:893482/1‑140 (MQ=255)
             tGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTcc                                                                                                                                <  1:1350348/132‑1 (MQ=255)
             tGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCg                                                                                                                        <  1:1174852/140‑1 (MQ=255)
              gTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCga                                                                                                                       >  2:459863/1‑140 (MQ=255)
                  aTCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGAcg                                                                                                                   >  1:417996/1‑140 (MQ=255)
                  aTCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGAcg                                                                                                                   >  2:1024123/1‑140 (MQ=255)
                    cagcagTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGAcgcg                                                                                                                 <  1:1331722/140‑1 (MQ=255)
                    cagcagTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGAcgcg                                                                                                                 <  1:296429/140‑1 (MQ=255)
                    cagcagTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGAcgcg                                                                                                                 <  2:460043/140‑1 (MQ=255)
                     agcagTTTGGCAATAATCGTATTTTCTCCTCACCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGAcgcgc                                                                                                                >  1:648773/1‑140 (MQ=255)
                      gcagTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGAcgcgcg                                                                                                               <  2:1097856/140‑1 (MQ=255)
                       cagTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCgg                                                                                                              >  1:1365563/1‑140 (MQ=255)
                       cagTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCgg                                                                                                              >  1:498448/1‑140 (MQ=255)
                       cagTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCgg                                                                                                              >  2:329126/1‑140 (MQ=255)
                        agTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGt                                                                                                             <  2:709413/140‑1 (MQ=255)
                         gTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGtt                                                                                                            >  2:1363864/1‑140 (MQ=255)
                         gTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGtt                                                                                                            <  1:620559/140‑1 (MQ=255)
                          tttGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCgag                                                                                                                      <  1:381335/129‑1 (MQ=255)
                            tGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTAtg                                                                                                         >  1:317638/1‑140 (MQ=255)
                            tGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTAtg                                                                                                         >  2:1467180/1‑140 (MQ=255)
                             ggCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTAtgt                                                                                                        <  2:1069328/140‑1 (MQ=255)
                              gCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTAtgtg                                                                                                       <  1:1200826/140‑1 (MQ=255)
                                   aatCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTgaagaa                                                                                                  >  1:926498/1‑140 (MQ=255)
                                    atCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAAc                                                                                                 >  2:1150758/1‑140 (MQ=255)
                                    atCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAAc                                                                                                 >  1:1406511/1‑140 (MQ=255)
                                       gTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTTGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTg                                                                                              <  1:64979/140‑1 (MQ=255)
                                       gTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTg                                                                                              <  1:630097/140‑1 (MQ=255)
                                       gTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTg                                                                                              <  1:984890/140‑1 (MQ=255)
                                       gTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTg                                                                                              <  1:1024123/140‑1 (MQ=255)
                                       gTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTg                                                                                              <  1:1217031/140‑1 (MQ=255)
                                       gTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTg                                                                                              <  2:549192/140‑1 (MQ=255)
                                       gTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTg                                                                                              <  1:1266458/140‑1 (MQ=255)
                                        tATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGc                                                                                             >  2:133800/1‑140 (MQ=255)
                                               tcctcAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTa                                                                                      >  2:211170/1‑140 (MQ=255)
                                                 ctcAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGGCGCGCGGTTATGTGAAGAACGTGCTGGCTTATg                                                                                    <  1:976700/140‑1 (MQ=255)
                                                 ctcAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATg                                                                                    <  2:1365563/140‑1 (MQ=255)
                                                 ctcAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATg                                                                                    <  1:1150758/140‑1 (MQ=255)
                                                 ctcAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATg                                                                                    <  2:1196488/140‑1 (MQ=255)
                                                 ctcAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATg                                                                                    >  2:891589/1‑140 (MQ=255)
                                                 ctcAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATg                                                                                    <  2:232939/140‑1 (MQ=255)
                                                  tcAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGa                                                                                   >  2:1228744/1‑140 (MQ=255)
                                                   cagcagCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGAc                                                                                  >  1:167743/1‑140 (MQ=255)
                                                    agcagcTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACg                                                                                 >  2:1591687/1‑140 (MQ=255)
                                                    agcagcTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACg                                                                                 <  2:1341835/140‑1 (MQ=255)
                                                      cagcTTATAACGCCGGACCAGGGCGGGCGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCt                                                                               >  1:1067523/1‑140 (MQ=255)
                                                         cTTATAACGCCGGACCAGGGCGGGTGTGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTtac                                                                            >  2:169931/1‑140 (MQ=255)
                                                         cTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTtac                                                                            >  1:853965/1‑140 (MQ=255)
                                                           tataACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTtacta                                                                          <  2:672001/140‑1 (MQ=255)
                                                              aaCGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACg                                                                                 <  2:1489212/130‑1 (MQ=255)
                                                              aaCGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACg                                                                                 >  1:1489212/1‑130 (MQ=255)
                                                              aaCGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCg                                                                       <  1:891589/140‑1 (MQ=255)
                                                              aaCGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCg                                                                       <  2:758546/140‑1 (MQ=255)
                                                              aaCGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCg                                                                       <  2:1199732/140‑1 (MQ=255)
                                                               aCGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGc                                                                      <  1:133800/140‑1 (MQ=255)
                                                                cGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCt                                                                     >  1:456471/1‑140 (MQ=255)
                                                                  ccGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTAt                                                                   >  1:974502/1‑140 (MQ=255)
                                                                   cGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGAc                                                                                  <  1:248867/124‑1 (MQ=255)
                                                                   cGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTAtt                                                                  >  1:1475208/1‑140 (MQ=255)
                                                                   cGGACCAGGGC‑GGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTAttt                                                                 >  1:1177357/1‑140 (MQ=255)
                                                                     gACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTc                                                                >  2:845158/1‑140 (MQ=255)
                                                                     gACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTc                                                                >  2:448400/1‑140 (MQ=255)
                                                                      aCCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCa                                                               >  1:53796/1‑140 (MQ=255)
                                                                       ccAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGGCGGGCGTATCGACGCAGTTGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACGACCGCTATTTCAt                                                              <  2:1067523/140‑1 (MQ=255)
                                                                        cAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATg                                                             <  2:1122907/140‑1 (MQ=255)
                                                                        cAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATg                                                             <  2:417996/140‑1 (MQ=255)
                                                                           ggCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATgggg                                                          >  1:1048412/1‑140 (MQ=255)
                                                                            gCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATggggg                                                         <  2:74688/140‑1 (MQ=255)
                                                                                gTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATaa                                                     >  1:1438629/1‑140 (MQ=255)
                                                                                  gCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAAc                                                   <  2:941916/140‑1 (MQ=255)
                                                                                  gCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAAc                                                   <  1:211170/140‑1 (MQ=255)
                                                                                    gAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCg                                                 <  1:169931/140‑1 (MQ=255)
                                                                                     aaCCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGa                                                <  1:1591687/140‑1 (MQ=255)
                                                                                          ggCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTGTGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTtg                                           >  2:1393764/1‑140 (MQ=255)
                                                                                           gCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCGTTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTtga                                          >  2:1273463/1‑140 (MQ=255)
                                                                                           gCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTtga                                          >  2:925186/1‑140 (MQ=255)
                                                                                                gCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAgc                                     >  2:260325/1‑140 (MQ=255)
                                                                                                gCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAgc                                     >  2:547262/1‑140 (MQ=255)
                                                                                                    cAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCa                                 <  1:1467180/140‑1 (MQ=255)
                                                                                                    cAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCa                                 <  1:459863/140‑1 (MQ=255)
                                                                                                    cAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCa                                 <  2:641416/140‑1 (MQ=255)
                                                                                                            ggCGTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGTTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATgg                         >  2:906465/1‑140 (MQ=255)
                                                                                                               gTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGa                      <  2:317638/140‑1 (MQ=255)
                                                                                                               gTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGa                      <  1:448400/140‑1 (MQ=255)
                                                                                                               gTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGa                      <  1:260325/140‑1 (MQ=255)
                                                                                                               gTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGa                      <  2:498448/140‑1 (MQ=255)
                                                                                                               gTATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGa                      <  2:167743/140‑1 (MQ=255)
                                                                                                                tATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGAc                     <  2:49772/140‑1 (MQ=255)
                                                                                                                tATCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGAc                     <  2:1250583/140‑1 (MQ=255)
                                                                                                                  tCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGAcgt                   <  1:893482/140‑1 (MQ=255)
                                                                                                                  tCGACGCAGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGAcgt                   <  2:1014764/140‑1 (MQ=255)
                                                                                                                         aGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACt            >  1:1542310/1‑140 (MQ=255)
                                                                                                                           tGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGa          >  1:1266528/1‑140 (MQ=255)
                                                                                                                               aTTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCg      <  1:191630/140‑1 (MQ=255)
                                                                                                                               aTTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCg      <  1:310821/140‑1 (MQ=255)
                                                                                                                               aTTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCg      <  1:913343/140‑1 (MQ=255)
                                                                                                                                 ttGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCa    <  2:974502/140‑1 (MQ=255)
                                                                                                                                   gTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACg  >  1:56561/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                      
GTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACG  >  NC_000913/4632408‑4632678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑