Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,911,176	Δ1 bp	coding (164/249 nt)	mazE ←	antitoxin of the MazF‑MazE toxin‑antitoxin system MazE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,911,175	0	A	.	100.0%	217.3 / NA	51	coding (165/249 nt)	mazE	antitoxin of the MazF‑MazE toxin‑antitoxin system MazE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (27/24);  total (27/24)

CAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCAGATTGAGCGCCTGCATTAACGTAGCCGGGATCCGCACCGCCGGTGA  >  NC_000913/2911040‑2911306                                                                                                                                        |                                                                                                                                    cAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCagcgt                                                                                                                                 <  1:351417/140‑5 (MQ=255)       cAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATAc                                                                                                                          <  1:241877/140‑1 (MQ=255)        aaCCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACg                                                                                                                         <  2:405230/140‑1 (MQ=255)         aCCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACgg                                                                                                                        >  2:903233/1‑140 (MQ=255)          cccAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACggg                                                                                                                       >  1:91308/1‑140 (MQ=255)           ccAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGc                                                                                                                      <  2:763538/140‑1 (MQ=255)           ccAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGc                                                                                                                      <  1:903233/140‑1 (MQ=255)           ccAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGc                                                                                                                      <  1:747497/140‑1 (MQ=255)               aTCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCtt                                                                                                                  <  1:174563/140‑1 (MQ=255)                    aTCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGc                                                                                                             <  1:504959/140‑1 (MQ=255)                     tCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCa                                                                                                            <  2:784198/140‑1 (MQ=255)                     tCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCa                                                                                                            <  1:353081/140‑1 (MQ=255)                     tCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCa                                                                                                            <  2:197776/140‑1 (MQ=255)                        cccATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTg                                                                                                         >  1:937399/1‑140 (MQ=255)                           atatCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCt                                                                                                      <  2:177202/140‑1 (MQ=255)                           atatCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCt                                                                                                      <  1:700272/140‑1 (MQ=255)                                gggTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCaata                                                                                                 <  2:547574/140‑1 (MQ=255)                                     cGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAAtaattaa                                                                                            >  1:173262/1‑140 (MQ=255)                                      gTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAAtaattaat                                                                                           <  2:722272/140‑1 (MQ=255)                                        aTCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAAttt                                                                                         >  1:817505/1‑140 (MQ=255)                                            gCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCa                                                                                     <  2:817505/140‑1 (MQ=255)                                            gCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCa                                                                                     >  2:462773/1‑140 (MQ=255)                                            gCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCa                                                                                     >  2:306604/1‑140 (MQ=255)                                            gCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCa                                                                                     <  1:477501/140‑1 (MQ=255)                                                accattaccaGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATcca                                                                                 >  2:842943/1‑140 (MQ=255)                                                accattaccaGACTTCCTTATCTTTCGGCTCTCCCCAATCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATcca                                                                                 >  1:635617/1‑140 (MQ=255)                                                     taccaGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAgg                                                                            <  2:467688/140‑1 (MQ=255)                                                      accaGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGt                                                                           <  1:169133/140‑1 (MQ=255)                                                               ccTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATcttca                                                                  >  1:744303/1‑140 (MQ=255)                                                               ccTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATcttca                                                                  >  1:647720/1‑140 (MQ=255)                                                                       ttCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTtcatca                                                          >  1:820882/1‑140 (MQ=255)                                                                         cGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTtcatcatc                                                        <  2:407101/140‑1 (MQ=255)                                                                         cGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTtcatcatc                                                        >  2:45905/1‑140 (MQ=255)                                                                         cGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTtcatcatc                                                        >  2:751231/1‑140 (MQ=255)                                                                                 cccAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCa                                                >  1:291190/1‑140 (MQ=255)                                                                                 cccAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCa                                                >  1:298577/1‑140 (MQ=255)                                                                                         atatTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCAGATTGAgc                                        >  1:470174/1‑140 (MQ=255)                                                                                           atTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCAGATTGAgcgc                                      >  2:264416/1‑140 (MQ=255)                                                                                             tctcGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCAGATTGAGCGCCt                                    >  2:366664/1‑140 (MQ=255)                                                                                               tcGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCAGATTGAGCGCCTGc                                  >  2:179497/1‑140 (MQ=255)                                                                                                      ggTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCAGATTGAGCGCCTGCATTAACg                           >  2:592743/1‑140 (MQ=255)                                                                                                      ggTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCAGATTGAGCGCCTGCATTAACg                           <  2:298577/140‑1 (MQ=255)                                                                                                      ggTTTTCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCAGATTGAGCGCCTGCATTAACg                           <  2:820882/140‑1 (MQ=255)                                                                                                           tCCGGCGTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCAGATTGAGCGCCTGCATTAACGTAGcc                      <  2:173262/140‑1 (MQ=255)                                                                                                                 gTGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCAGATTGAGCGCCTGCATTAACGTAGCCGGGATc                >  2:556859/1‑140 (MQ=255)                                                                                                                  tGATGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCAGATTGAGCGCCTGCATTAACGTAGCCGGGATcc               >  2:783835/1‑140 (MQ=255)                                                                                                                     tGTCGTTGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCAGATTGAGCGCCTGCATTAACGTAGCCGGGATCCGCa            <  2:291190/140‑1 (MQ=255)                                                                                                                          ttGACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCAGATTGAGCGCCTGCATTAACGTAGCCGGGATCCGCAccgcc       >  2:258680/1‑140 (MQ=255)                                                                                                                            gACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCGATCCACCAGGTCAATCTTCACTTCATCATCAATATTCAGATTGAGCGCCTGCATTAACGTAGCCGGGATCCGCACCGCCgg     >  1:504896/1‑140 (MQ=255)                                                                                                                            gACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCAGATTGAGCGCCTGCATTAACGTAGCCGGGATCCGCACCGCCgg     <  1:556859/140‑1 (MQ=255)                                                                                                                            gACCAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCAGATTGAGCGCCTGCATTAACGTAGCCGGGATCCGCACCGCCgg     >  2:723648/1‑140 (MQ=255)                                                                                                                               cAGTTCAGC‑AGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCAGATTGAGCGCCTGCATTAACGTAGCCGGGATCCGCACCGCCGGTGa  >  1:314535/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    CAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAATTTGCCATCCACCAGGTCAATCTTCACTTCATCATCAATATTCAGATTGAGCGCCTGCATTAACGTAGCCGGGATCCGCACCGCCGGTGA  >  NC_000913/2911040‑2911306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑