Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,879,829	Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp	coding (115‑120/360 nt)	yeaR ←	DUF1971 domain‑containing protein YeaR

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1879829 =	0 (0.000)	56 (1.010)	41/272	0.2	100%	coding (120/360 nt)	yeaR	DUF1971 domain‑containing protein YeaR
	?	NC_000913	2514274 =	NA (NA)					noncoding (2/1345 nt)	IS186	repeat region
*	?	NC_000913	= 1879834	0 (0.000)	40 (0.720)	32/270	0.6	100%	coding (115/360 nt)	yeaR	DUF1971 domain‑containing protein YeaR
	?	NC_000913	= 2515616	NA (NA)					noncoding (1344/1345 nt)	IS186	repeat region

GAAGGAGGGAAAAGGATGCTTCAAATCCCACAGAATTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACcc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1879963‑1879829 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCC  >  NC_000913/2514274‑2514414                                                                                                                                                                                                                                                                                      GAAGGAGGGAAAAGGATGCTTCAAATCCCACAGAATTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCAT                                                                                                                                          <  2:356867/140‑1   AGGAGGGAAAAGGATGCTTCAAATCCCACAGAATTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAA                                                                                                                                        >  2:526319/1‑140    GGAGGGAAAAGGATGCTTCAAATCCCACAGAATTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGTAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAG                                                                                                                                       >  2:411409/1‑140      AGGGAAAAGGATGCTTCAAATCCCACAGAATTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCG                                                                                                                                     >  1:351805/1‑140        GGAAAAGGATGCTTCAAATCCCACAGAATTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCT                                                                                                                                   >  2:172887/1‑140        GGAAAAGGATGCTTCAAATCCCACAGAATTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCT                                                                                                                                   >  2:406428/1‑140          AAAAGGATGCTTCAAATCCCACAGAATTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAA                                                                                                                                 <  1:642551/140‑1                  GCTTCAAATCCCACAGAATTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGG                                                                                                                         <  1:41207/140‑1                   CTTCAAATCCCACAGAATTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGT                                                                                                                        >  1:943390/1‑140                     TCAAATCCCACAGAATTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTG                                                                                                                      <  2:346045/140‑1                      CAAATCCCACAGAATTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGT                                                                                                                     <  1:184368/140‑1                        AATCCCACAGAATTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGG                                                                                                                   >  2:266258/1‑140                          TCCCACAGAATTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTA                                                                                                                 <  1:515636/140‑1                               CAGAATTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCCCCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACG                                                                                                            <  2:511770/140‑1                                   ATTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTG                                                                                                        >  2:554957/1‑140                                    TTATATTCATCCGCGCTAAACGCTTTTCTGAAATAACCAAACTGCCCCTGCCGGAATATTCGAACGTCATTTTGATAAAGGAACGCG‑CCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGAT                                                                                                      <  2:874654/140‑1                                    TTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGA                                                                                                       >  2:342963/1‑140                                    TTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGA                                                                                                       <  1:526319/140‑1                                     TATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGAT                                                                                                      <  2:31705/140‑1                                            ATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGTTATTACGCCTGATATGATTT                                                                                               >  1:643252/1‑140                                            ATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTT                                                                                               >  1:170696/1‑140                                            ATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTT                                                                                               >  1:401287/1‑140                                            ATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTT                                                                                               >  1:389799/1‑140                                            ATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTT                                                                                               >  1:30335/1‑140                                              ACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAA                                                                                             >  1:501662/1‑140                                                 CGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGACTTAACGT                                                                                          >  1:280454/1‑140                                                        CGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATG                                                                                   >  2:64387/1‑140                                                               CTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACT                                                                            <  2:943390/140‑1                                                               CTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACT                                                                            <  1:172887/140‑1                                                               CTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACT                                                                            <  1:529963/140‑1                                                               CTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACT                                                                            <  1:718333/140‑1                                                                     TAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACG                                                                      >  2:34888/1‑140                                                                                CACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAA                                                                  <  2:175169/133‑1                                                                                CACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAA                                                                  >  1:175169/1‑133                                                                                 ACCTGCCGGAATATTCGAACGTCATCTTGGTAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAG                                                          >  2:571494/1‑140                                                                                     GCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAAT                                                      >  2:190061/1‑140                                                                                      CCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATT                                                     <  2:351805/140‑1                                                                                        GGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCT                                                   >  1:400154/1‑140                                                                                           ATATTCGAACGTCATCTTGATAAATGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGC                                                <  2:562122/140‑1                                                                                           ATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAGCTGGTCAGCAATTCTGGC                                                <  2:497832/140‑1                                                                                           ATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGC                                                <  2:30335/140‑1                                                                                           ATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGC                                                <  2:519819/140‑1                                                                                           ATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGC                                                <  2:501662/140‑1                                                                                           ATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGC                                                <  2:643252/140‑1                                                                                            TATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGACTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCC                                               <  2:280454/140‑1                                                                                               TCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCAT                                            <  2:626464/140‑1                                                                                                     GTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGT                                      <  1:791988/140‑1                                                                                                        ATCTTGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAG                                   >  1:596567/1‑140                                                                                                            TGATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCG                               >  1:511667/1‑140                                                                                                              ATAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAA                             <  1:342963/140‑1                                                                                                               TAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAG                            <  1:206767/140‑1                                                                                                               TAAAGGAACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAG                            <  2:170696/140‑1                                                                                                                      ACGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGA                     <  1:411409/140‑1                                                                                                                       CGCGCCCGGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGAT                    >  1:536511/1‑140                                                                                                                              GGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGG             <  2:401287/140‑1                                                                                                                              GGGGGTTTACCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGG             >  1:564580/1‑140                                                                                                                                         CCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCC  <  2:619826/140‑1                                                                                                                                                                                                                                                                                      GAAGGAGGGAAAAGGATGCTTCAAATCCCACAGAATTATATTCATACGCGCTCAACGCCTTTCTGGAATAAACAAACTGCACCTGCCGGAATATTCGAACGTCATCTTGATAAAGGAACGCGCCCGGGGGTTTACcc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1879963‑1879829 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCC  >  NC_000913/2514274‑2514414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.