Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913888,0140TG34.9% 74.6 / 11.7 43noncoding (44/81 nt)rybBsmall regulatory RNA RybB
Reads supporting (aligned to +/- strand):  ref base T (7/21);  new base G (14/1);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.84e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTCTGCGGTTGTGCAAAAAAATTTCATTTTCAGGGCAACTTCAGTTTTATCCTAATC  >  NC_000913/887886‑888144
                                                                                                                                |                                                                                                                                  
tGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTg                                                                                                                                  <  2:820479/131‑1 (MQ=255)
tGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTg                                                                                                                                  >  1:820479/1‑131 (MQ=255)
 gCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCca                                                                                                                        <  1:259709/140‑1 (MQ=255)
  cATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCcac                                                                                                                       >  2:759605/1‑140 (MQ=255)
       tGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATggg                                                                                                                             >  1:326211/1‑129 (MQ=255)
       tGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATggg                                                                                                                             <  2:326211/129‑1 (MQ=255)
       tGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACaaaaa                                                                                                                  >  2:759735/1‑139 (MQ=255)
          cACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATggg                                                                                                               <  2:323415/140‑1 (MQ=255)
          cACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATggg                                                                                                               <  1:759605/140‑1 (MQ=255)
          cACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATggg                                                                                                               <  1:886188/140‑1 (MQ=255)
            ccgaccgTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATggggg                                                                                                             >  2:217540/1‑139 (MQ=255)
            ccgaccgTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGa                                                                                                             >  2:823413/1‑140 (MQ=255)
            ccgaccgTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAAAggggg                                                                                                             >  2:220182/1‑139 (MQ=255)
               accgTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACAt                                                                                                          <  1:172630/140‑1 (MQ=255)
               accgTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACAt                                                                                                          <  2:464393/140‑1 (MQ=255)
                             tCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAAAGGGGACATCAAAGAAAAGCAGt                                                                                            >  1:183217/1‑140 (MQ=255)
                               gcgcgAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTgg                                                                                          <  2:631085/140‑1 (MQ=255)
                                cgcgAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAATGGGGGCATCAAAAAAAAGAAGTggg                                                                                         >  2:930296/1‑139 (MQ=255)
                                 gcgAGGGGATTTTGAAGAAGGGTGAGAGGGTTGCAAGGTAGGAGGTAAGGTTTAGAAAACAAAAAACCCAACAACCTTGAACCGAAAAGGCGGGGGTTGTTGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCa                                                                                        <  2:128914/140‑1 (MQ=255)
                                 gcgAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCa                                                                                        <  1:823413/140‑1 (MQ=255)
                                 gcgAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCa                                                                                        <  1:930296/140‑1 (MQ=255)
                                 gcgAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCa                                                                                        <  1:953149/140‑1 (MQ=255)
                                 gcgAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCa                                                                                        <  1:572266/140‑1 (MQ=255)
                                 gcgAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCa                                                                                        <  2:9143/140‑1 (MQ=255)
                                   gaggagATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACt                                                                                      <  2:489853/140‑1 (MQ=255)
                                    aggagATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTa                                                                                     >  1:143024/1‑140 (MQ=255)
                                    aggagATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTa                                                                                     >  1:563291/1‑140 (MQ=255)
                                                    ggTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGccc                                                                     <  1:217540/140‑1 (MQ=255)
                                                     gTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAAAGGGGACATCAAAAAAAAGCAGTGGCACTAATTAAGACTGATGCCCt                                                                    >  2:95400/1‑140 (MQ=255)
                                                      ttGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTg                                                                   <  1:326004/140‑1 (MQ=255)
                                                         agagGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGGTGGGCTTCACAAAATAGGGGCATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCgg                                                                >  2:431310/1‑140 (MQ=255)
                                                                tGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGtt                                                         >  1:558229/1‑140 (MQ=255)
                                                                                    ttAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAAAAAAACAGTGGCACTAATTAAGACTGATGCCCTGCGGGAAAATTCTGCGGGTGTGCaaaaaaaa                                     >  2:435762/1‑139 (MQ=255)
                                                                                            cAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTCTGCGGTTGTGCAAAAAAATTTCAtttt                             >  1:344243/1‑140 (MQ=255)
                                                                                                 aaCCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTCTGCGGTTGTGCAAAAAAATTTCATTTTCAggg                        <  2:143024/140‑1 (MQ=255)
                                                                                                   cccATCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAATAGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTCTGCGGTTGTGCAAAAAAATTTCATTTTCAGGGCa                      >  2:496774/1‑140 (MQ=255)
                                                                                                      aTCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAACAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAATTCTGCGGTTGTGCAAAAAAAATTCATTTTTAGGGCAACt                   >  2:816042/1‑140 (MQ=255)
                                                                                                       tCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTCTGCGGTTGTGCAAAAAAATTTCATTTTCAGGGCAACtt                  <  1:435762/140‑1 (MQ=255)
                                                                                                       tCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTCTGCGGTTGTGCAAAAAAATTTCATTTTCAGGGCAACtt                  >  2:886616/1‑140 (MQ=255)
                                                                                                       tCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAATGGGGACATCAAAAAAAAACAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTTTGCGGTTGTGCAAAAAAATTTCATTTTCAGGGCAACtt                  >  2:911156/1‑140 (MQ=255)
                                                                                                           ccTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTCTGCGGTTGTGCAAAAAAATTTCATTTTCAGGGCAACTTCAGt              >  1:785488/1‑140 (MQ=255)
                                                                                                            cTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACT‑ATTAAGACTGATGCCCTGCGGAAAAGTTCTGCGGTTGTGCAAAAAAATTTCATTTTCAGGGCAACTTCAGttt            <  2:563291/140‑1 (MQ=255)
                                                                                                                aaCCGAAATGGCGGGGGTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTCTGCGGTTGTGCAAAAAAATTTCATTTTCAGGGCAACTTCAGTTTTAt         >  2:353014/1‑140 (MQ=255)
                                                                                                                      aaTGGCGGGGGTGGTTGGCTCCACAAAATGGGGACATCAAAGAAAAACAGGGGCACTAATTAAGACCGGTGCCCTGCGGAAAAGATCCGCGGTTGTGGAAAAAAATTTCATTTTCCGGGGAACTTAAGTTTTATCCTTAt   >  2:26856/1‑140 (MQ=255)
                                                                                                                       aTGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTCTGCGGTTGTGCAAAAAAATTTCATTTTCAGGGCAACTTCAGTTTTATCCTAATc  <  2:785488/140‑1 (MQ=255)
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TGCATTTTGTCACCGACCGTAAGCCGCTATCGCGCGAGGAGATTTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTCTGCGGTTGTGCAAAAAAATTTCATTTTCAGGGCAACTTCAGTTTTATCCTAATC  >  NC_000913/887886‑888144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑