Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT26.5% 59.9 / 13.3 34intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (2/23);  new base T (9/0);  total (11/23)
Fisher's exact test for biased strand distribution p-value = 1.05e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGA  >  NC_000913/3206302‑3206554
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cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACa                                                                                                                   <  1:870123/140‑1 (MQ=255)
   tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAAt                                                                                                                >  1:597035/1‑140 (MQ=255)
     ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATAc                                                                                                              <  1:648321/140‑1 (MQ=255)
      cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACa                                                                                                             <  1:177867/140‑1 (MQ=255)
               ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTTAGAcaaaaacaaaaaaagga                                                                                                    >  2:878317/1‑135 (MQ=255)
               ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTTAGACAAAAAAAAAAAATGGa                                                                                                    >  2:648321/1‑140 (MQ=255)
                      gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTggca                                                                                             <  2:597035/140‑4 (MQ=255)
                         atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatga                                                                                          <  2:562790/140‑1 (MQ=255)
                         atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatga                                                                                          <  1:259606/140‑1 (MQ=255)
                                ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTATTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                   <  1:470685/140‑1 (MQ=255)
                                    tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTTAGACAAAAAAAAAAAAAAGAGGTGCCCTGGTGAGGGAAAga                                                                               >  2:475779/1‑139 (MQ=255)
                                      ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaa                                                                             <  2:474123/140‑1 (MQ=255)
                                       tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                            <  1:590809/140‑1 (MQ=255)
                                                 aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                  <  1:296441/140‑1 (MQ=255)
                                                   tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                <  2:931867/140‑1 (MQ=255)
                                                      cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTg                                                             <  1:572416/140‑1 (MQ=255)
                                                        aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                           <  1:475779/140‑1 (MQ=255)
                                                        aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                           <  2:335283/140‑1 (MQ=255)
                                                         aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATGGAGGTGGCATGATGAGCGGAAATAAAAAAAAACAGGCAGTGaaa                                                          >  2:72126/1‑139 (MQ=255)
                                                             ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCACAAAAAAAAAAAAAATGGGGGTGGCCTGATGAGGGAAAAAAATAAGAAACAGGCAGAGAATAAga                                                      >  2:884349/1‑139 (MQ=255)
                                                             ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCATGATGAGCGAAAGAAATAAGCAACAAGCAGTGAaaaaat                                                      >  1:501135/1‑138 (MQ=255)
                                                                     atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCTAAAAAAAAAAAAAAAAT‑GAGATGGCAAGATGAGGGGAAGTAATAAAAAACAAGCAGGGAATAAGTTGACaaaa                                              >  2:119709/1‑137 (MQ=255)
                                                                       atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAAACAAAAACAAAAAACGGAGGTGGCAAGATGAGGGGAAAAAAAAAACAAAAAACAGAGAATAAATTGAAAgagaa                                            >  2:691546/1‑139 (MQ=255)
                                                                       atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAAACAAAAAAAAAAAATGGAGGTGCCCTGATGAGGGAAAAGAAAAAAAAAAAAGCAGGGAATAAATTTACAgagaa                                            >  2:41406/1‑139 (MQ=255)
                                                                       atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAACAAAAAATGGAGGTGGCATGATGAGCGAAAGGAAAAAAAAAAAGGCAGAGAAAAAATTGAAagaaaa                                            >  2:393105/1‑137 (MQ=255)
                                                                       atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                            <  1:878317/140‑1 (MQ=255)
                                                                            cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg                                       <  1:824467/140‑1 (MQ=255)
                                                                              gTGGCAAAAAATATAAAGCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                     <  1:287189/140‑1 (MQ=255)
                                                                              gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGGGTTGGCCGCATGAGGGAAAATAAAAAGCAACAAGAAAAGAAAAAATTTACAGAGATTGTTGcc                                     >  2:95692/1‑139 (MQ=255)
                                                                               tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAACAAAAAAAGGAGGTGCCATGATGAGGGAAAAGAAAAAACAAAAGGCAGAGAAAAAATTGAAAGAGAATGTCGCTa                                    >  2:160881/1‑140 (MQ=255)
                                                                               tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AATTTTTTCTGAAAAAAACAAAAAATGGGGGGTCCATTCTGTGCGCAAATAATAAAAAACAGGCAGTGAAAAAAGTGAAAAAGATTGTTGCTa                                    >  2:619940/1‑140 (MQ=255)
                                                                                 gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCA‑ACAAAAAAAAAAAAATGAGGTGGCCTGGTGAGGGGAAGAAAAAAACAAAAAGCAGGGAAAAAAATGACAGAGAATGTTGCTaaa                                  >  2:218814/1‑139 (MQ=255)
                                                                                   aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGGGGTGTCATGATGAGCGGAAGAAAAAAAAAAAAAACAGTGAAAAAATTGACAGAGATTGTCGCTAACtt                                >  2:29956/1‑140 (MQ=255)
                                                                                           taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAAAAAAAAAAATGGAGGTTCCATGATGAGGGGAAGTAATAAACAAAAAGCAGTGAATAAATTGACAGAGAATGTTGCTAACTTTACCGccc                        >  2:285814/1‑139 (MQ=255)
                                                                                               aCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑ATTTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAt                    <  2:501135/140‑1 (MQ=255)
                                                                                               aCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGCCGCTAACTTTACCGCCATGAt                    <  1:691546/140‑1 (MQ=255)
                                                                                               aCCACATTTTTAGTGGTAGTTTGTGGCGGTA‑AATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAt                    <  2:649012/140‑1 (MQ=255)
                                                                                                         tAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa          <  1:285814/140‑1 (MQ=255)
                                                                                                         tAGTGGTAGTTTGTGGCGGTA‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa          <  1:218814/140‑1 (MQ=255)
                                                                                                                 gTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtga  <  1:72126/140‑1 (MQ=255)
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CATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGA  >  NC_000913/3206302‑3206554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑