Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	607,090	G→T	G98G (GGC→GGA)	ybdK ←	carboxylate‑amine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	607,090	0	G	T	100.0%	91.4 / NA	30	G98G (GGC→GGA)	ybdK	carboxylate‑amine ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/12);  total (18/12)

ACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCAAACATCCGTCGCCAGCTCCAGCATACTTTCGGTGATAT  >  NC_000913/606968‑607226                                                                                                                           |                                                                                                                                         aCATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTc                                                                                                                         <  1:91176/140‑1 (MQ=255)     ggACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATCCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAga                                                                                                                     <  2:493894/140‑1 (MQ=255)        cATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAATTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAgatga                                                                                                                  <  1:419675/140‑1 (MQ=255)             tGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTc                                                                                                             >  1:187403/1‑140 (MQ=255)             tGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTc                                                                                                             >  2:545249/1‑140 (MQ=255)               aCCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAACGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCtg                                                                                                           >  1:134421/1‑140 (MQ=255)                  aaaaaGGGTCGCCTGCTGAAGGAGATACCCAAAGTTTTCCAGCGTGGGTTGATAGCGTTTGTTATCCCATCCCTCCTGACGCTGCCTTTTCTGAACCGGGTGCGTCCCACCGCCGCAAATTTCCAGATGATGGTCTGTgg                                                                                                        <  2:325830/140‑1 (MQ=255)                     aaCGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAgatg                                                                                                                   >  2:669303/1‑126 (MQ=255)                     aaCGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAgatg                                                                                                                   <  1:669303/126‑1 (MQ=255)                            gCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCaa                                                                                              >  2:800211/1‑140 (MQ=255)                                    aaTGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACtt                                                                                      >  2:283266/1‑140 (MQ=255)                                        agaTAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTg                                                                                  <  1:299898/140‑1 (MQ=255)                                          aTAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATCCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCa                                                                                <  1:737024/140‑1 (MQ=255)                                          aTAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCTTTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCa                                                                                <  1:137963/140‑1 (MQ=255)                                            aaCCAAAGTTTTCCAGCGTGCGTTGATAGCGTCCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAACCGGGGGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATc                                                                              <  2:132982/140‑1 (MQ=255)                                              ccAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATCCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCa                                                                                <  2:763460/136‑1 (MQ=255)                                              ccAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCa                                                                                >  1:763460/1‑136 (MQ=255)                                              ccAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGc                                                                            >  2:449927/1‑140 (MQ=255)                                                        ccAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGc                                                                  >  2:176436/1‑140 (MQ=255)                                                         cAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGcc                                                                 >  2:206189/1‑140 (MQ=255)                                                            cgtgcgtTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCgg                                                              <  1:206189/140‑1 (MQ=255)                                                              tgcgtTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCa                                                            >  2:408792/1‑140 (MQ=255)                                                              tgcgtTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCa                                                            >  2:925035/1‑140 (MQ=255)                                                                 gtTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCTTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGcc                                                         <  2:118578/140‑1 (MQ=255)                                                                        gCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGa                                                  >  2:38793/1‑140 (MQ=255)                                                                           ttcgttATCGCATCCCTCCTGACGCTGCATTTTCTGAAACGGGTGCGTCCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGatat                                               <  1:64167/140‑1 (MQ=255)                                                                           ttcgttATCGCATCCCCCCTGACGCTGCATTTTCTGAAGCGGGTGCGTCCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGatat                                               <  2:134421/140‑1 (MQ=255)                                                                           ttcgttACGCCATTCTTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCTAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGatat                                               <  1:573943/140‑1 (MQ=255)                                                                                        aCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCAAACATc                                  >  2:233547/1‑140 (MQ=255)                                                                                        aCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCAAACATc                                  >  2:764664/1‑140 (MQ=255)                                                                                              tGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCAAACATCCGTCGc                            <  1:324711/140‑1 (MQ=255)                                                                                                    tGCTATTTCGGAACGGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGCCTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCAAACATCCGTCGCCAGCTc                      <  2:187403/140‑1 (MQ=255)                                                                                                    tGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCAAACATCCGTCGCCAGCTc                      >  2:251716/1‑140 (MQ=255)                                                                                                    tGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCAAACATCCGTCGCCAGCTc                      >  2:531106/1‑140 (MQ=255)                                                                                                         tttCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCAAACATCCGTCGCCAGCTCCAGCa                 <  1:233547/140‑1 (MQ=255)                                                                                                                aaCGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAATCTGCCCGGCAGCCTGGTTGATATCACGGCAAACATCCGTCGCCAGCTCCAGCATACTTTc          >  1:7795/1‑140 (MQ=255)                                                                                                                aaCGGGCGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCAAACATCCGTCGCCAGCTCCAGCATACTTTc          >  2:54082/1‑140 (MQ=255)                                                                                                                     gtgcgttcCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCAAACATCCGTCGCCAGCTCCAGCATACTTTCGGTGa     >  1:792357/1‑140 (MQ=255)                                                                                                                        cgttcCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCAAACATCCGTCGCCAGCTCCAGCATACTTTCGGTGatat  <  1:408792/140‑1 (MQ=255)                                                                                                                           |                                                                                                                                         ACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCAAACATCCGTCGCCAGCTCCAGCATACTTTCGGTGATAT  >  NC_000913/606968‑607226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑