Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,995,683	T→C	pseudogene (338/707 nt)	ygeN ←	putative type III secretion system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,995,683	0	T	C	100.0%	334.9 / NA	88	pseudogene (338/707 nt)	ygeN	putative type III secretion system protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/42);  total (46/42)

TATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAAT  >  NC_000913/2995550‑2995819                                                                                                                                      |                                                                                                                                         tatGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATa                                                                                                                                    <  1:643086/140‑1 (MQ=255)   tGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATaaa                                                                                                                                  <  2:509391/140‑1 (MQ=255)     gACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATaaaaa                                                                                                                                >  2:702519/1‑140 (MQ=255)      aCATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAAc                                                                                                                               >  1:458066/1‑140 (MQ=255)       cATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACa                                                                                                                              <  2:831886/140‑1 (MQ=255)        aTTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACAt                                                                                                                             >  1:134757/1‑140 (MQ=255)        aTTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACAt                                                                                                                             >  1:699630/1‑140 (MQ=255)        aTTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACAt                                                                                                                             >  1:883991/1‑140 (MQ=255)         ttCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATc                                                                                                                            <  1:638949/140‑1 (MQ=255)          tCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCa                                                                                                                           >  2:869840/1‑140 (MQ=255)          tCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCa                                                                                                                           >  2:377626/1‑140 (MQ=255)          tCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCa                                                                                                                           >  2:932905/1‑140 (MQ=255)           ccATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCaa                                                                                                                          >  2:304617/1‑140 (MQ=255)            cATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAAc                                                                                                                         <  2:832596/140‑1 (MQ=255)             atatTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAAct                                                                                                                        <  1:615624/140‑1 (MQ=255)               atTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAActtc                                                                                                                      >  1:237709/1‑140 (MQ=255)               atTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAActtc                                                                                                                      >  1:231936/1‑140 (MQ=255)                  gATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACttcttc                                                                                                                   >  1:36529/1‑140 (MQ=255)                  gATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACttcttc                                                                                                                   >  2:36758/1‑140 (MQ=255)                    tttGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTg                                                                                                                 >  1:821356/1‑140 (MQ=255)                    tttGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTg                                                                                                                 <  2:688166/140‑1 (MQ=255)                    tttGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTg                                                                                                                 <  1:293272/140‑1 (MQ=255)                    tttGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTg                                                                                                                 >  1:413163/1‑140 (MQ=255)                     ttGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAACCATCAACTTCTTCTGa                                                                                                                <  2:739394/140‑1 (MQ=255)                        tCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAgg                                                                                                             >  2:257965/1‑140 (MQ=255)                          gACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGtt                                                                                                           >  1:753582/1‑140 (MQ=255)                           aCAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGttt                                                                                                          <  2:586882/140‑1 (MQ=255)                           aCAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGttt                                                                                                          <  2:807926/140‑1 (MQ=255)                            cAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGtttt                                                                                                         <  2:29764/140‑1 (MQ=255)                             aaaaTATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTa                                                                                                        <  2:702216/140‑1 (MQ=255)                             aaaaTATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTa                                                                                                        <  2:146585/140‑1 (MQ=255)                                atatGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACa                                                                                                     <  2:36529/140‑1 (MQ=255)                                   tGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAata                                                                                                  <  2:231936/140‑1 (MQ=255)                                     aCTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAAtata                                                                                                <  2:883991/140‑1 (MQ=255)                                     aCTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAAtata                                                                                                <  1:869840/140‑1 (MQ=255)                                      ctctACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATAc                                                                                               >  2:829083/1‑140 (MQ=255)                                      ctctACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATAc                                                                                               >  2:793946/1‑140 (MQ=255)                                       tctACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATAct                                                                                              >  2:464607/1‑140 (MQ=255)                                         tACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATActct                                                                                            >  1:691107/1‑140 (MQ=255)                                          aCTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCtt                                                                                           >  2:115619/1‑140 (MQ=255)                                           cTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTc                                                                                          >  1:608083/1‑140 (MQ=255)                                            ttCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATGCTCTTCa                                                                                         <  1:691101/140‑1 (MQ=255)                                              cAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAGCAATATACTCTTCAGa                                                                                       >  1:457981/1‑140 (MQ=255)                                               aaGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGAt                                                                                      <  1:702519/140‑1 (MQ=255)                                                agagACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATc                                                                                     <  1:377626/140‑1 (MQ=255)                                                       tATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATc                                                                              <  2:134757/140‑1 (MQ=255)                                                        aTCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCa                                                                             >  1:653100/1‑140 (MQ=255)                                                             tGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGttt                                                                        >  1:627959/1‑140 (MQ=255)                                                                aaCTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTAtt                                                                     >  1:482125/1‑140 (MQ=255)                                                                     gtaggtaTATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCtttt                                                                >  1:949884/1‑140 (MQ=255)                                                                     gtaggtaTATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCtttt                                                                <  1:36758/140‑1 (MQ=255)                                                                          tataTGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTc                                                           <  2:753582/140‑1 (MQ=255)                                                                               ggAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGtt                                                      >  2:557556/1‑140 (MQ=255)                                                                               ggAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGtt                                                      >  2:857410/1‑140 (MQ=255)                                                                               ggAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGtt                                                      <  1:115619/140‑1 (MQ=255)                                                                                   aTTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGa                                                  >  1:49390/1‑140 (MQ=255)                                                                                   aTTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGa                                                  >  1:895171/1‑140 (MQ=255)                                                                                   aTTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGa                                                  >  2:102853/1‑140 (MQ=255)                                                                                    tttACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGtt                                                      <  2:156896/135‑1 (MQ=255)                                                                                    tttACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGtt                                                      >  1:156896/1‑135 (MQ=255)                                                                                    tttACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAg                                                 >  1:379555/1‑140 (MQ=255)                                                                                    tttACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAg                                                 >  2:769234/1‑140 (MQ=255)                                                                                    tttACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAg                                                 <  2:653100/140‑1 (MQ=255)                                                                                    tttACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAg                                                 >  1:647094/1‑140 (MQ=255)                                                                                    tttACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAg                                                 <  1:932905/140‑1 (MQ=255)                                                                                    tttACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAg                                                 <  2:34495/140‑1 (MQ=255)                                                                                    tttACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAg                                                 <  1:857410/140‑1 (MQ=255)                                                                                          ggACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGc                                           <  2:413163/140‑1 (MQ=255)                                                                                           gACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCa                                          <  2:949884/140‑1 (MQ=255)                                                                                             ccTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAg                                        >  1:738660/1‑140 (MQ=255)                                                                                             ccTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAg                                        >  2:895589/1‑140 (MQ=255)                                                                                                gATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCtt                                     >  1:914868/1‑140 (MQ=255)                                                                                                   atTGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATc                                  <  1:869983/140‑1 (MQ=255)                                                                                                    ttGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCa                                 <  2:237709/140‑1 (MQ=255)                                                                                                     tGATGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCaa                                <  2:49390/140‑1 (MQ=255)                                                                                                       aTGGTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAgg                              >  1:733610/1‑140 (MQ=255)                                                                                                          gTAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGta                           <  1:557556/140‑1 (MQ=255)                                                                                                           tAATTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGtat                          >  1:377198/1‑140 (MQ=255)                                                                                                             aTTTTGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATaa                        <  2:482125/140‑1 (MQ=255)                                                                                                                ttGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCCTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAAt                     >  1:804362/1‑140 (MQ=255)                                                                                                                 tGTTACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAAtt                    >  2:166939/1‑140 (MQ=255)                                                                                                                   ttACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGta                           >  1:888201/1‑131 (MQ=255)                                                                                                                   ttACCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGta                           <  2:888201/131‑1 (MQ=255)                                                                                                                     aCCCAACTTTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCCTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATcc                <  2:804362/140‑1 (MQ=255)                                                                                                                          aCTCTCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCAtctc           <  2:895171/140‑1 (MQ=255)                                                                                                                              tCAAAAATCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCtcatca       >  2:371612/1‑140 (MQ=255)                                                                                                                                   aaTCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCTAGGGTATAAAATTATCCATCTCATCaataat  <  1:464607/140‑1 (MQ=255)                                                                                                                                   aaTCTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCaataat  <  2:738660/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAAT  >  NC_000913/2995550‑2995819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑