Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,760,398	C→A	A284A (GCG→GCT)	selA ←	selenocysteine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,760,398	0	C	A	100.0%	184.5 / NA	54	A284A (GCG→GCT)	selA	selenocysteine synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/27);  total (27/27)

CATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑CGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTA  >  NC_000913/3760263‑3760534                                                                                                                                         |                                                                                                                                         cATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCc‑agccg                                                                                                                                      <  1:777539/140‑1 (MQ=255)  aTTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑agccgc                                                                                                                                     >  1:736281/1‑140 (MQ=255)   ttttATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCa                                                                                                                                    <  1:945873/140‑1 (MQ=255)              cgTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTTATCCCTTTTTTACCCACAATAATTCCTGCCCGCGGGCCGCCCGACAAATTTTCGGCTGAGAAACCCACCCGACTGAGGCCAACCCGCAA‑CAACTCCTg                                                                                                                         >  2:182585/1‑140 (MQ=255)              cgTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTg                                                                                                                         <  2:902868/140‑1 (MQ=255)              cgTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTg                                                                                                                         <  2:260610/140‑1 (MQ=255)                   tGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCACAATCAACTCCTGCGGCa                                                                                                                    <  1:646841/140‑1 (MQ=255)                      aCGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTg                                                                                                                 <  2:141334/140‑1 (MQ=255)                       cGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTgg                                                                                                                <  2:736281/140‑1 (MQ=255)                       cGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTgg                                                                                                                >  1:67371/1‑140 (MQ=255)                             aGCGGGTGGCTTTGCAGGCGGGCGAACATCTCTTTTTTACCAACAATAATTCCTGCCCGCGGCCCGCCTAACAACTTGTCGCCCGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCttt                                                                                                          >  2:112772/1‑140 (MQ=255)                                    ggCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCaaa                                                                                                   >  1:315960/1‑140 (MQ=255)                                    ggCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCaaa                                                                                                   >  2:827789/1‑140 (MQ=255)                                    ggCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCaaa                                                                                                   >  1:636305/1‑140 (MQ=255)                                         tGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                              <  1:827789/140‑1 (MQ=255)                                         tGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                              <  1:79093/140‑1 (MQ=255)                                         tGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                              <  1:182585/140‑1 (MQ=255)                                         tGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                              <  1:193940/140‑1 (MQ=255)                                         tGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                              <  2:636305/140‑1 (MQ=255)                                         tGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                              <  2:783129/140‑1 (MQ=255)                                         tGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                              <  1:490762/140‑1 (MQ=255)                                              gcgggcgATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGc                                                                                         <  2:529581/140‑1 (MQ=255)                                              gcgggcgATCATCCCTTTTTTCCCAACAATAATCCCTGCCTGCGGCCCGCCTAACATCTTGTCGCCGGAGAAATTCCCCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTGATTCGGCAAACCGTACTGGc                                                                                         <  2:241425/140‑1 (MQ=255)                                                        atcTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGAc                                                                               >  1:438540/1‑140 (MQ=255)                                                             ttttttACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGAGGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCg                                                                          >  1:928056/1‑140 (MQ=255)                                                              tttttACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTTTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGa                                                                         >  2:596162/1‑140 (MQ=255)                                                                tttACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGc                                                                       >  1:8893/1‑140 (MQ=255)                                                                tttACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTACTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGc                                                                       >  2:870958/1‑140 (MQ=255)                                                                 ttACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGcc                                                                      <  2:451738/140‑1 (MQ=255)                                                                  tACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTTACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCa                                                                     >  2:568247/1‑140 (MQ=255)                                                                  tACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCACCTCCTGCGGCATTGTCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGGGCCa                                                                     <  2:542143/140‑1 (MQ=255)                                                                  tACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCa                                                                     <  2:672966/140‑1 (MQ=255)                                                                  tACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCa                                                                     <  1:326076/140‑1 (MQ=255)                                                                  tACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCa                                                                     >  2:138422/1‑140 (MQ=255)                                                                   aCCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCAc                                                                    >  2:776865/1‑140 (MQ=255)                                                                       acaaTAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGcc                                                                >  1:942662/1‑140 (MQ=255)                                                                        caaTAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCt                                                               <  2:315960/140‑1 (MQ=255)                                                                                   gcctgcGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCAc                                                    >  2:272104/1‑140 (MQ=255)                                                                                   gcctgcGGCCAGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTc                                                      >  1:69828/1‑138 (MQ=255)                                                                                   gcctgcGGCCAGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTc                                                      <  2:69828/138‑1 (MQ=255)                                                                                     ctgcGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTa                                                  >  2:632797/1‑140 (MQ=255)                                                                                      tgcGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAAt                                                           >  1:257001/1‑130 (MQ=255)                                                                                      tgcGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAAt                                                           <  2:257001/130‑1 (MQ=255)                                                                                        cGGTCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACgg                                               >  1:28469/1‑140 (MQ=255)                                                                                               ccTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATc                                        <  2:28469/140‑1 (MQ=255)                                                                                                          gTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGc                             >  2:445540/1‑140 (MQ=255)                                                                                                          gTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGc                             >  1:799278/1‑140 (MQ=255)                                                                                                          gTCGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGc                             >  1:50549/1‑140 (MQ=255)                                                                                                            cGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCg                           >  1:264545/1‑140 (MQ=255)                                                                                                            cGCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCg                           <  1:445540/140‑1 (MQ=255)                                                                                                             gCCGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGa                          <  2:722179/140‑1 (MQ=255)                                                                                                              ccGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAg                         <  1:276047/140‑1 (MQ=255)                                                                                                              ccGGAGAAACTCACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAg                         >  2:105650/1‑140 (MQ=255)                                                                                                                         cACCAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGtt              <  2:71384/140‑1 (MQ=255)                                                                                                                           ccAGACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTcc            <  1:713823/140‑1 (MQ=255)                                                                                                                              gACTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCt         <  1:776865/140‑1 (MQ=255)                                                                                                                               aCTGACTCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCAACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCtt        <  2:928056/140‑1 (MQ=255)                                                                                                                                cTGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTc       >  1:905734/1‑140 (MQ=255)                                                                                                                                 tGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCa      >  2:138977/1‑140 (MQ=255)                                                                                                                                 tGACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCa      >  2:124215/1‑140 (MQ=255)                                                                                                                                  gACGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCAt     >  1:372168/1‑140 (MQ=255)                                                                                                                                   aCGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATc    <  2:275195/140‑1 (MQ=255)                                                                                                                                   aCGCC‑AGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATc    <  1:138977/140‑1 (MQ=255)                                                                                                                                    cgcc‑agccGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCt   >  2:187336/1‑140 (MQ=255)                                                                                                                                     gcc‑agccGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTa  <  1:124215/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCC‑CGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTA  >  NC_000913/3760263‑3760534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑